The purpose of this project is to generate updated WHO estimates, for 2016, of the global and WHO regional prevalence and incidence of herpes simplex virus (HSV) type 1 and HSV-2 infection. To do this, we undertook a literature review of published HSV-1 and HSV-2 infection prevalence and incidence, to identify data newly-published since the last time the global HSV infection estimates were produced. An Excel spreadsheet of the extracted data from the literature review, and the Appendix to the manuscript for the project, are required by the publisher of the manuscript (Bulletin of the World Health Organization) to be deposited in an online data repository for public access.