Proteomics Facility

Facility/equipment: Facility

  • Location

    Proteomics Facility, Faculty of Life Sciences

    Biomedical Sciences Building

    Bristol

    BS8 1TD

    United Kingdom

Research Output

  • 51 Article (Academic Journal)
  • 1 Letter (Academic Journal)
2019

Transduction with BBF2H7/CREB3L2 upregulates SEC23A protein in erythroblasts and partially corrects the hypo-glycosylation phenotype associated with CDAII

Pellegrin, S., Haydn-Smith, K., Hampton-O'Neil, L., Hawley, B., Heesom, K., Fermo, E., Bianchi, P. & Toye, A., 1 Mar 2019, In : British Journal of Haematology. 184, 5, p. 876-881 6 p.

Research output: Contribution to journalLetter (Academic Journal)

Open Access
File
1 Citation (Scopus)
194 Downloads (Pure)
2018

Assessment of the red blood cell proteome in a dog with unexplained hemolytic anemia

Black, V. L., Heesom, K., Whittington, F., Davis, S., Tasker, S., Adamantos, S. E. & Davidson, A. D., 1 Sep 2018, In : Veterinary Clinical Pathology. 47, 3, p. 377-385 9 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
106 Downloads (Pure)

Cancer cell adaptation to hypoxia involves a HIF-GPRC5A-YAP axis

Greenhough, A., Bagley, C., Heesom, K., Gurevich, D., Gay, D., Bond, M., Collard, T., Paraskeva, C., Martin, P., Sansom, O. J., Malik, K. & Williams, A., Aug 2018, In : EMBO Molecular Medicine. 10, 8, 13 p., e8699.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
12 Citations (Scopus)
215 Downloads (Pure)
Open Access
File
4 Citations (Scopus)
237 Downloads (Pure)

Dynein-2 intermediate chains play crucial but distinct roles 2 in primary cilia formation and function

Vuolo, L., Stevenson, N., Heesom, K. & Stephens, D., 16 Oct 2018, In : eLife. 7, 29 p., e39655.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
8 Citations (Scopus)
217 Downloads (Pure)

Dysregulation of mesenchymal stromal cell antioxidant responses in progressive multiple sclerosis

Redondo, J., Sarkar, P., Kemp, K., Heesom, K., Wilkins, A., Scolding, N. & Rice, C., Oct 2018, In : Stem Cells Translational Medicine. 7, 10, p. 748-758 11 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
8 Citations (Scopus)
274 Downloads (Pure)

Enhancement of red blood cell transfusion compatibility using CRISPR‐mediated erythroblast gene editing

Hawksworth, J., Satchwell, T., Meinders, M., Daniels, D., Regan, F., Thornton, N., Wilson, M., Dobbe, J. G. G., Streekstra, G. J., Trakarnsanga, K., Heesom, K., Anstee, D., Frayne, J. & Toye, A., 26 Apr 2018, In : EMBO Molecular Medicine. 10, 5, 11 p., e8454.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
12 Citations (Scopus)
242 Downloads (Pure)

Envelope proteome changes driven by RamA overproduction in Klebsiella pneumoniae that enhance acquired β-lactam resistance

Jiménez-Castellanos, J-C., Wan Ahmad Kamil, W. N. I., Takebayashi, Y., Findlay, J., Schneiders, T., Heesom, K. J. & Avison, M. B., 1 Jan 2018, In : Journal of Antimicrobial Chemotherapy. 73, 1, p. 88-94 7 p., dkx345.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
11 Citations (Scopus)
247 Downloads (Pure)

Epistasis analysis uncovers hidden antibiotic resistance-associated fitness costs hampering the evolution of MRSA

Yokoyama, M., Stevens, E., Laabei, M., Bacon, L., Heesom, K., Bayliss, S., Ooi, N., O'Neill, A. J., Murray, E., Williams, P., Lubben, A., Reeksting, S., Meric, G., Pascoe, B., Sheppard, S. K., Recker, M., Hurst, L. D. & Massey, R. C., 18 Jul 2018, In : Genome Biology. 19, 12 p., 94.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
9 Citations (Scopus)
152 Downloads (Pure)

Impact of OqxR loss of function on the envelope proteome of Klebsiella pneumoniae and susceptibility to antimicrobials

Wan Ahmad Kamil, W. N. I., Takebayashi, Y., Findlay, J., Heesom, K. & Avison, M., 1 Nov 2018, In : Journal of Antimicrobial Chemotherapy. 73, 11, p. 2990-2996 7 p., dky293.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
4 Citations (Scopus)
106 Downloads (Pure)

Isoform-specific AMPK association with TBC1D1 is reduced by a mutation associated with severe obesity

Thomas, E., Hook, S. C., Grey, A., Chadt, A., Carling, D., Al-Hasani, H., Heesom, K. J., Hardie, G. & Tavare, J., Sep 2018, In : Biochemical Journal. 475, 18, p. 2969-2983 15 p., 475.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
5 Citations (Scopus)
250 Downloads (Pure)

Prediction of fluoroquinolone susceptibility directly from whole-genome sequence data by using liquid chromatography-tandem mass spectrometry to identify mutant genotypes

Wan Ahmad Kamil, W. N. I., Takebayashi, Y., Findlay, J., Heesom, K. J., Jiménez-Castellanos, J. C., Zhang, J., Graham, L., Bowker, K., Williams, O. M., MacGowan, A. P. & Avison, M. B., 1 Mar 2018, In : Antimicrobial Agents and Chemotherapy. 62, 3, p. e01814-01817 4 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
3 Citations (Scopus)
177 Downloads (Pure)

Preliminary evidence of reductive stress in human cytotoxic T-cells following exercise

Wadley, A. J., Holliday, A., Morgan, R. G., Heesom, K. J., Aldred, S., Peters, D. M., Bueno, A. A. & Coles, S. J., 12 Apr 2018, In : Journal of Applied Physiology.

Research output: Contribution to journalArticle (Academic Journal)

4 Citations (Scopus)

Quantitative proteomics of plasma vesicles identify novel biomarkers for Hemoglobin E/ β-Thalassemic patients

Kittivorapart, J., Karamatic Crew, V., Wilson, M., Heesom, K., Toye, A. & Siritanaratkul, N., Feb 2018, In : Blood Advances. 2, 2, p. 95-104 10 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
4 Citations (Scopus)
289 Downloads (Pure)

Secretory factors from OP9 stromal cells delay differentiation and increase the expansion potential of adult erythroid cells in vitro

Trakarnsanga, K., Wilson, M. C., Heesom, K. J., Andrienko, T. N., Srisawat, C. & Frayne, J., 31 Jan 2018, In : Scientific Reports. 8, 1, 8 p., 1983.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
3 Citations (Scopus)
208 Downloads (Pure)

SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion

McGough, I. J., de Groot, R. E. A., Jellett, A. P., Betist, M. C., Varandas, K. C., Danson, C. M., Heesom, K. J., Korswagen, H. C. & Cullen, P. J., 13 Sep 2018, In : Nature Communications. 9, 13 p., 3737.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
11 Citations (Scopus)
245 Downloads (Pure)

Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin

Danson, C., Pearson, N., Heesom, K. & Cullen, P., 10 Sep 2018, In : Journal of Cell Science. 14 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
233 Downloads (Pure)
2017

BASP1 interacts with estrogen receptor α and modifies the tamoxifen response

Marsh, L., Carrera, S., Shandilya, J., Heesom, K., Davidson, A., Medler, K. F. & Roberts, S., 11 May 2017, In : Cell Death and Disease. 8, 5, 10 p., e2771.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
6 Citations (Scopus)
185 Downloads (Pure)

Comparison of protein expression during wild-type, and E1B-55k-deletion, adenovirus infection using quantitative time-course proteomics

Fu, Y. R., Turnell, A. S., Davis, S., Heesom, K. J., Evans, V. C. & Matthews, D. A., 20 Jun 2017, In : Journal of General Virology. 98, 6, p. 1377-1388 12 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
7 Citations (Scopus)
194 Downloads (Pure)

Heightened autoantibody immune response to citrullinated calreticulin in bronchiectasis: implications for rheumatoid arthritis

Clarke, A., Perry, E., Kelly, C., De Soyza, A., Heesom, K., Gold, L. I., Ollier, W., Hutchinson, D. & Eggleton, P., Aug 2017, In : International Journal of Biochemistry and Cell Biology. 89, p. 199-206 8 p.

Research output: Contribution to journalArticle (Academic Journal)

4 Citations (Scopus)

In-depth PtdIns(3,4,5)P3 signalosome analysis identifies DAPP1 as a negative regulator of GPVIdriven platelet function

Durrant, T., Hutchinson, L., Heesom, K., Anderson, K., Stephens, L., Hawkins, P., Marshall, A., Moore, S. & Hers, I., 13 Jun 2017, In : Blood Advances. 1, 14, p. 918-932 14 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
14 Citations (Scopus)
318 Downloads (Pure)

Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors

Sorgeloos, F., Caddy, S. L., Vashist, S., Sosnovtsev, S., Lloyd, R., Heesom, K. J., Locker, N. & Goodfellow, I., 1 Apr 2017, In : Molecular and Cellular Proteomics. 16, 4, p. S215-S229 15 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
16 Citations (Scopus)
280 Downloads (Pure)

P4HB recurrent missense mutation causing Cole-Carpenter syndrome

Balasubramanian, M., Padidela, R., Pollitt, R. C., Bishop, N. J., Mughal, M. Z., Offiah, A. C., Wagner, B. E., McCaughey, J. & Stephens, D. J., 19 Dec 2017, In : Journal of Medical Genetics. 9 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
10 Citations (Scopus)
186 Downloads (Pure)

Proteomics Informed by Transcriptomics for Characterising Active Transposable Elements and Genome Annotation in Aedes Aegypti

Maringer, K., Yousuf, A., Heesom, K., Fan, J., Lee, D., Fernandez-Sesma, A., Bessant, C., Matthews, D. & Davidson, A., 19 Jan 2017, In : BMC Genomics. 18, 18 p., 101.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
17 Citations (Scopus)
327 Downloads (Pure)

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling

McNally, K. E., Faulkner, R., Steinberg, F., Gallon, M., Ghai, R., Pim, D., Langton, P., Pearson, N., Danson, C. M., Nägele, H., Morris, L. L., Singla, A., Overlee, B. L., Heesom, K. J., Sessions, R., Banks, L., Collins, B. M., Berger, I., Billadeau, D. D., Burstein, E. & 1 others, Cullen, P. J., 1 Oct 2017, In : Nature Cell Biology. 19, 10, p. 1214–1225 12 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
73 Citations (Scopus)
260 Downloads (Pure)

Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50

Kvainickas, A., Jimenez Orgaz, A., Nägele, H., Diedrich, B., Heesom, K., Dengjel, J., Cullen, P. & Steinberg, F., 15 Jan 2017, In : Journal of Cell Science. 130, p. 382-395 19 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
151 Downloads (Pure)
Open Access
File
47 Citations (Scopus)
317 Downloads (Pure)

Structural/mechanistic insights into the efficacy of nonclassical β-lactamase inhibitors against extensively drug resistant Stenotrophomonas maltophilia clinical isolates

Calvopina Tapia, K., Hinchliffe, P., Brem, J., Heesom, K. J., Johnson, S., Cain, R., Lohans, C. T., Fishwick, C. W. G., Schofield, C. J., Spencer, J. & Avison, M. B., 23 Oct 2017, In : Molecular Microbiology. 106, 3, p. 492–504 13 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
17 Citations (Scopus)
263 Downloads (Pure)

The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3)-binder Rasa3 Regulates Phosphoinositide 3-kinase (PI 3-kinase)-dependent Integrin αIIbβ3 Outside-in Signaling

Battram, A., Durrant, T., Agbani, E., Heesom, K., Paul, D. S., Piatt, R., Poole, A., Cullen, P., Bergmeier, W., Moore, S. & Hers, I., 3 Feb 2017, In : Journal of Biological Chemistry. 292, 5, p. 1691-1704 14 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
15 Citations (Scopus)
244 Downloads (Pure)
2016

Atypical parkinsonism-associated retromer mutant alters endosomal sorting of specific cargo proteins

McMillan, K. J., Gallon, M. J., Jellett, A. P., Clairfeuille, T., Tilley, F. C., McGough, I., Danson, C. M., Heesom, K. J., Wilkinson, K. A., Collins, B. M. & Cullen, P. J., 15 Aug 2016, In : Journal of Cell Biology. 214, 4, p. 389-399 11 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
20 Citations (Scopus)
406 Downloads (Pure)

Calcium-insensitive splice variants of mammalian E1 subunit of 2-oxoglutarate dehydrogenase complex with tissue-specific patterns of expression

Denton, D., Pullen, T. J., Armstrong, C. T., Heesom, K. J. & Rutter, G. A., May 2016, In : Biochemical Journal. 473, p. 1165-1178 14 p., 9.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
16 Citations (Scopus)
321 Downloads (Pure)

Comparative effects of overproducing the AraC-type transcriptional regulators MarA, SoxS, RarA and RamA on antimicrobial drug susceptibility in Klebsiella pneumoniae

Jiménez-Castellanos, J-C., Wan Ahmad Kamil, W. N. I., Cheung, C. H. P., Tobin, M. S., Brown, J., Isaac, S. G., Heesom, K. J., Schneiders, T. & Avison, M. B., 7 Jul 2016, In : Journal of Antimicrobial Chemotherapy. 71, 7, p. 1820-1825 6 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
15 Citations (Scopus)
355 Downloads (Pure)

Comparison of the proteome of adult and cord erythroid cells, and changes in the proteome following reticulocyte maturation

Wilson, M., Trakarnsanga, K., Heesom, K., Toye, A. & Frayne, J., 1 Jun 2016, In : Molecular and Cellular Proteomics. 15, 6, p. 1938-1946 9 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
48 Citations (Scopus)
331 Downloads (Pure)

Disease causing mutations in inverted formin 2 regulate its binding to G-actin, F-actin capping protein (CapZ α-1) and profilin 2

Rollason, R., Wherlock, M. D., Heath, J. A., Heesom, K. J., Saleem, M. & Welsh, G. I., Feb 2016, In : Bioscience Reports. 36, 1, 11 p., e00302.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
8 Citations (Scopus)
414 Downloads (Pure)

Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton

Zuccala, E. S., Satchwell, T. J., Angrisano, F., Tan, Y. H., Wilson, M. C., Heesom, K. J. & Baum, J., 2 Feb 2016, In : Scientific Reports. 6, 16 p., 19766 .

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
24 Citations (Scopus)
236 Downloads (Pure)

TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion

McCaughey, J., Miller, V. J., Stevenson, N., Brown, A., Budnik, A., Heesom, K., Alibhai, D. R. & Stephens, D., 24 May 2016, In : Cell Reports. 15, 8, p. 1648-59 12 p., 15.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
28 Citations (Scopus)
371 Downloads (Pure)
2015

Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance

Hamdollah Zadeh, M. A., Amin, E. M., Hoareau-Aveilla, C., Domingo, E., Symonds, K. E., Ye, X., Heesom, K. J., Salmon, A., D'Silva, O., Betteridge, K. B., Williams, A. C., Kerr, D. J., Salmon, A. H. J., Oltean, S., Midgley, R. S., Ladomery, M. R., Harper, S. J., Varey, A. H. R. & Bates, D. O., Jan 2015, In : Molecular Oncology. 9, 1, p. 167-78 12 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
35 Citations (Scopus)
56 Downloads (Pure)

Galaxy Integrated Omics: Web-based standards-compliant workflows for proteomics informed by transcriptomics

Fan, J., Saha, S., Barker, G., Heesom, K. J., Ghali, F., Jones, A. R., Matthews, D. A. & Bessant, C., 12 Aug 2015, In : Molecular and Cellular Proteomics. 14, 11, p. 3087-3093 7 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
22 Citations (Scopus)
249 Downloads (Pure)

Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells

Traylen, C., Ramasubramanyan, S., Zuo, J., Rowe, M., Almohammad, R., Heesom, K., Sweet, S. M. M., Matthews, D. A. & Sinclair, A. J., 29 Oct 2015, In : Pathogens. 4, 4, p. 739-51 13 p.

Research output: Contribution to journalArticle (Academic Journal)

8 Citations (Scopus)

Protein arginine methyltransferase 5 is a key regulator of the MYCN oncoprotein in neuroblastoma cells

Park, J. H., Szemes, M., Vieira, G. C. C., Melegh, Z. B., Malik, S., Heesom, K. J., Von Wallwitz-Freitas, L., Greenhough, A., Brown, K. W., Zheng, Y. G., Catchpoole, D., Deery, M. J. & Malik, K. T. A., Mar 2015, In : Molecular Oncology. 9, 3, p. 617-27 11 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
21 Citations (Scopus)
228 Downloads (Pure)
2014

Analysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics

Alqahtani, A., Heesom, K., Bramson, J. L., Curiel, D., Ugai, H. & Matthews, D. A., Nov 2014, In : Journal of General Virology. 95, Pt 11, p. 2504-11 8 p.

Research output: Contribution to journalArticle (Academic Journal)

9 Citations (Scopus)

Cardiac phosphoproteomics during remote ischemic preconditioning: a role for the sarcomeric Z-disk proteins

Abdul-Ghani, S., Heesom, K. J., Angelini, G. D., Suleiman, M-S. & Suleiman, M. S., 2014, In : BioMed Research International. 2014, 767812.

Research output: Contribution to journalArticle (Academic Journal)

18 Citations (Scopus)

Hearts from mice fed a non-obesogenic high-fat diet exhibit changes in their oxidative state, calcium and mitochondria in parallel with increased susceptibility to reperfusion injury

Littlejohns, B., Pasdois, P., Duggan, S., Bond, A. R., Heesom, K., Jackson, C. L., Angelini, G. D., Halestrap, A. P. & Suleiman, M. S., 2014, In : PLoS ONE. 9, 6, p. e100579

Research output: Contribution to journalArticle (Academic Journal)

38 Citations (Scopus)

High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells

Chiu, H-C., Hannemann, H., Heesom, K. J., Matthews, D. A. & Davidson, A. D., 26 Mar 2014, In : PLoS ONE. 9, 3, p. e93305 22 p., 93305.

Research output: Contribution to journalArticle (Academic Journal)

31 Citations (Scopus)

Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL-mediated apoptosis

Wynne, J. W., Shiell, B. J., Marsh, G. A., Boyd, V., Harper, J. A., Heesom, K., Monaghan, P., Zhou, P., Payne, J., Klein, R., Todd, S., Mok, L., Green, D., Bingham, J., Tachedjian, M., Baker, M. L., Matthews, D. & Wang, L-F., 2014, In : Genome Biology. 15, 11, p. 532

Research output: Contribution to journalArticle (Academic Journal)

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

Trakarnsanga, K., Wilson, M. C., Griffiths, R. E., Toye, A. M., Carpenter, L., Heesom, K. J., Parsons, S. F., Anstee, D. J. & Frayne, J., 2014, In : PLoS ONE. 9, 7, p. e100874

Research output: Contribution to journalArticle (Academic Journal)

24 Citations (Scopus)

Retromer binding to FAM21 and the WASH complex is perturbed by the Parkinson disease-linked VPS35(D620N) mutation

McGough, I. J., Steinberg, F., Jia, D., Barbuti, P. A., McMillan, K. J., Heesom, K. J., Whone, A. L., Caldwell, M. A., Billadeau, D. D., Rosen, M. K. & Cullen, P. J., 21 Jul 2014, In : Current Biology. 24, 14, p. 1670-1676 7 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
90 Citations (Scopus)
261 Downloads (Pure)

Retromer binding to FAM21 and the WASH complex is perturbed by the Parkinson disease-linked VPS35(D620N) mutation: Erratum

McGough, I. J., Steinberg, F., Jia, D., Barbuti, P. A., McMillan, K. J., Heesom, K. J., Whone, A. L., Caldwell, M. A., Billadeau, D. D., Rosen, M. K. & Cullen, P. J., 21 Jul 2014, In : Current Biology. 24, 14, p. 1678 1 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
80 Downloads (Pure)

The effect of disease on human cardiac protein expression profiles in paired samples from right and left ventricles

Littlejohns, B., Heesom, K., Angelini, G. D. & Suleiman, M-S., 2014, In : Clinical Proteomics. 11, 1, p. 34

Research output: Contribution to journalArticle (Academic Journal)

10 Citations (Scopus)
2013

A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.

Steinberg, F., Gallon, M. J., Winfield, M. O., Thomas, E. C., Bell, A. J., Heesom, K. J., Tavare, J. M. & Cullen, P. J., May 2013, In : Nature Cell Biology. 15, 5, p. 461-471 11 p.

Research output: Contribution to journalArticle (Academic Journal)

234 Citations (Scopus)