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Personal profile

Research interests

The Molecular Neueroendocrinology Research Group uses gene discovery and transfer techniques to study the neuronal regulation of the cardiovascular system in health and disease. Interests have focussed on hypothalamic neurons involved in the maintenance of plasma osmolality, and the integration of autonomic cardiovascular signalling by brainstem (nucleus of the solitary tract; NTS) neurons (in collaboration with Dr Julian Paton, Dept of Physiology, University of Bristol).

We are using microarray, differential screening and yeast two-hybrid methodologies to identify genes expressed in hypothalamic and brainstem neurons, and genes which alter their pattern of expression following physiological challenges. The hypotheses that emerge from the analysis of expression information must be tested with genetic methods involving the transfer of genes into model organisms. We have accumulated considerable experience with the generation and analysis of transgenic models; both germline systems mediated by the microinjection of fertilised one-cell eggs with cloned DNA fragments, and somatic systems mediated by the stereotaxic injection of Adenoviral gene transfer vectors into specific brain regions. Current strategies involve combining gene discovery with precise real-time physiological measurements.

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Projects

Research Output

Transcription factor Creb3l1 regulates the synthesis of prohormone convertase enzyme PC1/3 in endocrine cells

Greenwood, M., Paterson, A., Rahman, P. A., Gillard, B. T., Langley, S., Iwasaki, Y., Murphy, D. & Greenwood, M. P., 28 Apr 2020, In : Journal of Neuroendocrinology. 32, 4, 15 p., E12851.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
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  • 25 Downloads (Pure)

    Vasopressin & oxytocin in control of the cardiovascular system: An updated review

    Japundžić-žigon, N., Lozić, M., Šarenac, O. & Murphy, D., 1 Jan 2020, In : Current Neuropharmacology. 18, 1, p. 14-33 20 p.

    Research output: Contribution to journalBook/Film/Article review (Academic Journal)

  • 2 Citations (Scopus)

    Vasopressin and v1br gene expression is increased in the hypothalamic pvn of borderline hypertensive rats

    Savić, B., Martin, A., de Souza Mecawi, A., Bukumirić, Z., Antunes-Rodrigues, J., Murphy, D., Sarenac, O. & Japundžić–Žigon, N., 15 May 2020, In : Hypertension Research. 2020, 10 p.

    Research output: Contribution to journalArticle (Academic Journal)

  • Activities

    Integrative Neuroscience and Endocrinology Showcase: Dorothy Hodgkin Building 10th Anniversary Symposium

    David Murphy (Participant)

    21 Jan 2015

    Activity: Participating in or organising an event typesParticipation in conference

    Third Symposium Miguel Ozorio de Almeida

    David Murphy (Participant)

    12 May 201515 May 2015

    Activity: Participating in or organising an event typesParticipation in conference

    American Journal of Physiology (Regulatory, Integrative and Comparative Physiology) (External organisation)

    David Murphy (Member)

    2015

    Activity: Membership typesMembership or peer review panel or committee