Projects per year
The Molecular Neueroendocrinology Research Group uses gene discovery and transfer techniques to study the neuronal regulation of the cardiovascular system in health and disease. Interests have focussed on hypothalamic neurons involved in the maintenance of plasma osmolality, and the integration of autonomic cardiovascular signalling by brainstem (nucleus of the solitary tract; NTS) neurons (in collaboration with Dr Julian Paton, Dept of Physiology, University of Bristol).
We are using microarray, differential screening and yeast two-hybrid methodologies to identify genes expressed in hypothalamic and brainstem neurons, and genes which alter their pattern of expression following physiological challenges. The hypotheses that emerge from the analysis of expression information must be tested with genetic methods involving the transfer of genes into model organisms. We have accumulated considerable experience with the generation and analysis of transgenic models; both germline systems mediated by the microinjection of fertilised one-cell eggs with cloned DNA fragments, and somatic systems mediated by the stereotaxic injection of Adenoviral gene transfer vectors into specific brain regions. Current strategies involve combining gene discovery with precise real-time physiological measurements.
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The neurohumoral control of body fluid and cardiovascular homeostasis in males and females - vive la difference!
1/12/19 → 31/12/19
Advancing respiratory–cardiovascular physiology with the working heart–brainstem preparation over 25 yearset al., 16 Mar 2022, In: The Journal of Physiology. 600, 9, p. 2049-2075 27 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile3 Citations (Scopus)35 Downloads (Pure)
Transcriptional and Post-Transcriptional Regulation of Oxytocin and Vasopressin Gene Expression by CREB3L1 and CAPRIN2Bárez-López, S., Konopacka, A., Cross, S. J., Greenwood, M., Skarveli, M., Murphy, D. & Greenwood, M. P., 20 Jan 2022, (E-pub ahead of print) In: Neuroendocrinology. 112, 11, p. 1058-1077 20 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile1 Citation (Scopus)61 Downloads (Pure)
Greenwood, M., Gillard, B. T., Farrukh, R., Paterson, A., Althammer, F., Grinevich, V., Murphy, D. & Greenwood, M. P., 1 Sep 2022, In: Molecular metabolism. 63, p. 1-17 101542.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile4 Downloads (Pure)
Data from: Improving Illumina assemblies with Hi-C and long reads: an example with the North African dromedary
Elbers, J. P. (Contributor), Rogers, M. F. (Contributor), Perelman, P. L. (Contributor), Proskuryakova, A. A. (Contributor), Serdyukova, N. A. (Contributor), Johnson, W. E. (Contributor), Horin, P. (Contributor), Corander, J. (Contributor), Murphy, D. (Contributor) & Burger, P. A. (Contributor), Dryad, 27 Mar 2019
BBSRC Japan Partnering Award (with Dr Mike Ludwig, Prof John Russell, Prof Gareth Leng, Dr Tatsushi Onaka, Dr Yoichi Ueta). “Applying gene transfer technologies to study dendritic neuropeptide release mechanisms and functions”. JPA1304
Murphy, David (Recipient), 2004
Prize: Prizes, Medals, Awards and Grants
BBSRC USA Partnering Award (with Profs Jonathan Sweedler and Martha Gillette, University of Illinois). “Novel insights into the mechanisms of salt appetite”. (BB/J01981X/1)
Murphy, David (Recipient), 2012
Prize: Prizes, Medals, Awards and Grants
Integrative Neuroscience and Endocrinology Showcase: Dorothy Hodgkin Building 10th Anniversary Symposium
David Murphy (Participant)21 Jan 2015
Activity: Participating in or organising an event types › Participation in conference
American Journal of Physiology (Regulatory, Integrative and Comparative Physiology) (External organisation)
David Murphy (Member)2015
Activity: Membership types › Membership or peer review panel or committee