Projects per year
The Molecular Neueroendocrinology Research Group uses gene discovery and transfer techniques to study the neuronal regulation of the cardiovascular system in health and disease. Interests have focussed on hypothalamic neurons involved in the maintenance of plasma osmolality, and the integration of autonomic cardiovascular signalling by brainstem (nucleus of the solitary tract; NTS) neurons (in collaboration with Dr Julian Paton, Dept of Physiology, University of Bristol).
We are using microarray, differential screening and yeast two-hybrid methodologies to identify genes expressed in hypothalamic and brainstem neurons, and genes which alter their pattern of expression following physiological challenges. The hypotheses that emerge from the analysis of expression information must be tested with genetic methods involving the transfer of genes into model organisms. We have accumulated considerable experience with the generation and analysis of transgenic models; both germline systems mediated by the microinjection of fertilised one-cell eggs with cloned DNA fragments, and somatic systems mediated by the stereotaxic injection of Adenoviral gene transfer vectors into specific brain regions. Current strategies involve combining gene discovery with precise real-time physiological measurements.
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1/09/18 → 31/08/22
The neurohumoral control of body fluid and cardiovascular homeostasis in males and females - vive la difference!
1/12/19 → 31/12/19
Corticosterone pattern-dependent glucocorticoid receptor binding and transcriptional regulation within the liverFlynn, B. P., Birnie, M. T., Kershaw, Y. M., Pauza, A. G., Kim, S., Baek, S., Rogers, M. F., Paterson, A. R., Stavreva, D. A., Murphy, D., Hager, G. L., Lightman, S. L. & Conway-Campbell, B. L., 10 Aug 2021, In: PLoS Genetics. 17, 8, p. e1009737 28 p., e1009737.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile23 Downloads (Pure)
Transcriptome Analysis Reveals Downregulation of Urocortin Expression in the Hypothalamo-Neurohypophysial System of Spontaneously Hypertensive RatsMartin, A., Mecawi, A. S., Antunes, V. R., Yao, S. T., Antunes-Rodrigues, J., Paton, J. F. R., Paterson, A., Greenwood, M., Šarenac, O., Savić, B., Japundžić-Žigon, N., Murphy, D. & Hindmarch, C. C. T., 17 Mar 2021, In: Frontiers in Physiology. 11, 18 p., 599507.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile3 Downloads (Pure)
Transcription factor Creb3l1 regulates the synthesis of prohormone convertase enzyme PC1/3 in endocrine cellsGreenwood, M., Paterson, A., Rahman, P. A., Gillard, B. T., Langley, S., Iwasaki, Y., Murphy, D. & Greenwood, M. P., 28 Apr 2020, In: Journal of Neuroendocrinology. 32, 4, 15 p., E12851.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile6 Citations (Scopus)84 Downloads (Pure)
BBSRC Japan Partnering Award (with Dr Mike Ludwig, Prof John Russell, Prof Gareth Leng, Dr Tatsushi Onaka, Dr Yoichi Ueta). “Applying gene transfer technologies to study dendritic neuropeptide release mechanisms and functions”. JPA1304
Murphy, David (Recipient), 2004
Prize: Prizes, Medals, Awards and Grants
BBSRC USA Partnering Award (with Profs Jonathan Sweedler and Martha Gillette, University of Illinois). “Novel insights into the mechanisms of salt appetite”. (BB/J01981X/1)
Murphy, David (Recipient), 2012
Prize: Prizes, Medals, Awards and Grants
Integrative Neuroscience and Endocrinology Showcase: Dorothy Hodgkin Building 10th Anniversary Symposium
David Murphy (Participant)21 Jan 2015
Activity: Participating in or organising an event types › Participation in conference
American Journal of Physiology (Regulatory, Integrative and Comparative Physiology) (External organisation)
David Murphy (Member)2015
Activity: Membership types › Membership or peer review panel or committee