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Personal profile

Research interests

The primary basic research interests of the group are: (A) the informational aspect of the protein-folding problem; that is, how does the sequence of a protein determine its active, three-dimensional structure or fold?  And (B), how can we use this information to design completely new proteins from scratch?  In addition, we design proteins for applications in synthetic biology and medicine.

We tackle these problems using the following multi-disciplinary approach:

  1. We use bioinformatics to garner sequence-to-structure relationships from protein sequence and structural databases.

  2. We test the relationships ("rules for protein folding") that we find in two ways: (a) through ab initio protein-structure prediction; and (b) via rational protein design, where we engineer natural protein structures, or design new ones completely from scratch (so-called de novo design).

  3. We then test our engineered and design proteins experimentally using biophysical methods. The peptides and proteins are made either by peptide synthesis, or via recombinant DNA methods and the expression of synthetic genes. The products and then characterised using methods including: solution-phase biophysics (CD, FT-IR and fluorescence spectroscopy, AUC and ITC); high-resolution structural biology (NMR spectroscopy and X-ray crystallography); and microscopy (EM, AFM and light microscopy).

  4. Finally, we explore potential applications of some the engineered and designed proteins in the burgeoning fields of bionanotechnology and synthetic biology.

Professor Woolfson is a supervisor in the EPSRC Centre for Doctoral Training in Chemical Synthesis

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Projects

Research Output

Self-assembling cages from coiled-coil peptide modules

Fletcher, J. M., Harniman, R. L., Barnes, F. R. H., Boyle, A. L., Collins, A. M., Mantell, J., Sharp, T. H., Antognozzi, M., Booth, P. J., Linden, N., Miles, M. J., Sessions, R. B., Verkade, P. & Woolfson, D. N., 3 May 2013, In : Science. 340, 6132, p. 595-599 5 p.

Research output: Contribution to journalArticle (Academic Journal)

  • 300 Citations (Scopus)

    Rational design and application of responsive α-helical peptide hydrogels

    Banwell, EF., Abelardo, ES., Adams, DJ., Birchall, MA., Corrigan, A., Donald, AM., Kirkland, M., Serpell, LC., Butler, MF. & Woolfson, DN., Jul 2009, In : Nature Materials. 8, p. 596-600 5 p.

    Research output: Contribution to journalArticle (Academic Journal)

  • 333 Citations (Scopus)

    n→π* interactions in proteins

    Bartlett, G. J., Choudhary,, A., Raines, R. T. & Woolfson, D. N., Jul 2010, In : Nature Chemical Biology. 6, p. 615 - 620 6 p.

    Research output: Contribution to journalArticle (Academic Journal)

  • 222 Citations (Scopus)

    Datasets

    Activities

    • 1 Participation in conference
    • 1 Participation in workshop, seminar, course

    Inaugural Bristol BioDesign Institute Conference

    Kathleen R Sedgley (Participant), , Dek Woolfson (Organiser), , Imre Berger (Organiser), , Katharina Landfester (Keynote/plenary speaker), & Nadrian Seeman (Keynote/plenary speaker)

    10 May 2018

    Activity: Participating in or organising an event typesParticipation in conference

    File

    Computationally Directed Protein Design Workshop

    Dek Woolfson (Participant), , Fabio Parmeggiani (Participant), , Ross Anderson (Participant), , Kathleen Sedgley (Participant), & Tom Bowker (Participant)

    2018

    Activity: Participating in or organising an event typesParticipation in workshop, seminar, course

    Thesis

    Conformation Prediction of Synthetic Ion Channels: From structure prediction to structure classification

    Author: Aguilar Sanjuan, B., 28 Nov 2019

    Supervisor: Woolfson, D. (Supervisor) & Liverpool, T. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy (PhD)

    File

    De novo designed protein-protein interaction domains for synthetic biology applications in cells

    Author: Edgell, C. L., 23 Jan 2019

    Supervisor: Woolfson, D. (Supervisor) & Savery, N. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy (PhD)

    File

    Design, synthesis and characterisation of new protein folds

    Author: Porter Goff, K., 19 Mar 2019

    Supervisor: Woolfson, D. (Supervisor), Race, P. (Supervisor) & Booker-Milburn, K. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy (PhD)

    File