Dek N Woolfson

2024

A de novo designed coiled coil-based switch regulates the microtubule motor kinesin-1

Cross, J. A. A., Dawson, W. M., Shukla, S. R., Weijman, J. F. M. S., Mantell, J. M., Dodding, M. P. & Woolfson, D. N., Jul 2024, In: Nature Chemical Biology. 20, 7, p. 916-923 8 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

An atlas of protein homo-oligomerization across domains of life

Schweke, H., Pacesa, M., Levin, T., Kumar, P., Duhoo, Y., Dornfeld, L. J., Dubreuil, B., Georgeon, S., A. Goverde, C., Ovchinnikov, S., Woolfson, D. N., Correia, B. E., Dey, S. & Levy, E. D., 6 Feb 2024, In: Cell. 187, 4, p. 999-1010.e15 27 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

8 Citations (Scopus)

Assembling membraneless organelles from de novo designed proteins

Hilditch, A. T., Romanyuk, A., Cross, S. J., Obexer, R., McManus, J. J. & Woolfson, D. N., 1 Jan 2024, In: Nature Chemistry. 16, 1, p. 89-97 9 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

10 Citations (Scopus)

56 Downloads (Pure)

Labile assembly of a tardigrade protein induces biostasis

Sanchez-Martinez, S., Nguyen, K., Biswas, S., Nicholson, V., Romanyuk, A. V., Ramirez, J., Kc, S., Akter, A., Childs, C., Meese, E. K., Usher, E. T., Ginell, G. M., Yu, F., Gollub, E., Malferrari, M., Francia, F., Venturoli, G., Martin, E. W., Caporaletti, F., Giubertoni, G., & 5 othersWoutersen, S., Sukenik, S., Woolfson, D. N., Holehouse, A. S. & Boothby, T. C., 1 Apr 2024, In: Protein science : a publication of the Protein Society. 33, 4, 39 p., e4941.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

2 Citations (Scopus)

Maturation and Conformational Switching of a De Novo Designed Phase-Separating Polypeptide

Hilditch, A., Romanyuk, A., Hodgson, L. R., Mantell, J. M., Neal, C. R., Verkade, P., Obexer, R. J., Serpell, L. C., McManus, J. & Woolfson, D. N., 17 Apr 2024, In: Journal of the American Chemical Society. 146, 15, p. 10240–10245 6 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

Rationally seeded computational protein design of ɑ-helical barrels

Albanese, K. I., Petrenas, R., Pirro, F., Naudin, E. A., Borucu, U., Dawson, W. M., Scott, D. A., Leggett, G. J., Weiner, O., Oliver, T. A. A. & Woolfson, D. N., 1 Aug 2024, In: Nature Chemical Biology. 20, 8, p. 991-999 9 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

Understanding β-strand mediated protein-protein interactions: tuning binding behaviour of intrinsically disordered sequences by backbone modification

Cawood, E. E., Baker, E., Edwards, T. A., Woolfson, D. N., Karamanos, T. K. & Wilson, A. J., 14 Jul 2024, In: Chemical Science. 15, 26, p. 10237-10245 9 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

2023

Allosteric regulation of a molecular motor through de novo protein design

Cross, J. A. A., Dawson, W. M., Shukla, S. R., Weijman, J. F. M. S., Mantell, J. M., Dodding, M. P. & Woolfson, D. N., 17 Oct 2023, In: bioRxiv.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

CC+: A Searchable Database of Validated Coiled Coils in PDB Structures and AlphaFold2 Models

Kumar, P., Petrenas, R., Dawson, W. M., Schweke, H., Levy, E. D. & Woolfson, D. N., 28 Sept 2023, (E-pub ahead of print) In: Protein Science. 32, 11, e4789.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

1 Citation (Scopus)

Design and Selection of Heterodimerizing Helical Hairpins for Synthetic Biology

Smith, A. J., Naudin, E. A., Edgell, C. L., Baker, E. G., Mylemans, B., FitzPatrick, L., Herman, A., Rice, H. M., Andrews, D., Tigue, N., Woolfson, D. N. & Savery, N. J., 16 Jun 2023, In: Journal of the American Chemical Society. 12, 6, p. 1845-1858 14 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

1 Citation (Scopus)

Differential sensing with arrays of de novo designed peptide assemblies

Dawson, W. M., Shelley, K. L., Fletcher, J. M., Scott, D. A., Lombardi, L., Rhys, G. G., LaGambina, T. J., Obst, U., Burton, A. J., Cross, J. A., Davies, G., Martin, F. J. O., Wiseman, F. J., Brady, R. L., Tew, D., Wood, C. W. & Woolfson, D. N., 24 Jan 2023, In: Nature Communications. 14, 1, 383.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

11 Citations (Scopus)

64 Downloads (Pure)

Evaluation and deployment of isotype-specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults

Thomas, A., Oliver, E. H., Baum, H. E., Gupta, K., Shelley, K. L., Long, A. E., Jones, H. E., Smith, J., Hitchings, B. E., Di Bartolo, N. D., Vasileiou, K., Rabi, F. A., Alamir, H. I. A., Eghleilib, M. A., Francis, O., Oliver, J. L., Morales-Aza, B., Obst, U., Shattock, D. J., Barr, R. S., & 21 othersCollingwood, L., Duale, K., Grace, N., Gonnage Livera, G., Bishop, L. C., Downing, H. E., Rodrigues, F., Timpson, N. J., Relton, C. L., Toye, A. M., Woolfson, D. N., Berger, I., Goenka, A., Davidson, A. D., Gillespie, K. M., Williams, A. J. K., Bailey, M., Brooks Pollock, E., Finn, A. H. R., Halliday, A. & Study Team, CM., 15 Mar 2023, In: Communications Medicine. 3, 1, 14 p., 37.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

Molecular Mechanism of STIL Coiled-Coil Domain Oligomerization

Shamir, M., Martin, F., Woolfson, D. N. & Friedler, A., 27 Sept 2023, In: International Journal of Molecular Sciences. 24, 19, 14 p., 14616.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

Rational Design of Phosphorylation-Responsive Coiled Coil-Peptide Assemblies

Thompson, H. F., Beesley, J. L., Langlands, H. D., Edgell, C. L., Savery, N. J. & Woolfson, D. N., 29 Mar 2023, In: ACS Synthetic Biology. 12, 4, p. 1308–1319 12 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

2 Citations (Scopus)

Understanding a protein fold: the physics, chemistry, and biology of α-helical coiled coils

Woolfson, D. N., 3 Mar 2023, In: Journal of Biological Chemistry. 299, 4, 104579.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

26 Citations (Scopus)

X-ray structure of the metastable SEPT14–SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization

Cavini, I. A., Winter, A. J., D'Muniz Pereira, H., Woolfson, D. N., Crump, M. P. & Garratt, R., 25 Sept 2023, In: Acta Crystallographica Section D: Structural Biology. D79, p. 881–894 14 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

1 Citation (Scopus)

2022

De novo designed peptides for cellular delivery and subcellular localisation

Rhys, G. G., Cross, J. A., Dawson, W. M., Thompson, H. F., Shanmugaratnam, S., Savery, N. J., Dodding, M. P., Höcker, B. & Woolfson, D. N., 1 Sept 2022, In: Nature Chemical Biology. 18, 9, p. 999-1004 6 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

15 Citations (Scopus)

222 Downloads (Pure)

De novo design of discrete, stable 310-helix peptide assemblies

Kumar, P., Paterson, N. G., Clayden, J. & Woolfson, D. N., 14 Jul 2022, In: Nature. 607, 7918, p. 387-392 11 p., 607.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

25 Citations (Scopus)

122 Downloads (Pure)

Development and evaluation of low-volume tests to detect and characterize antibodies to SARS-CoV-2

Halliday, A., Long, A. E., Baum, H. E., Thomas, A., Shelley, K. L., Oliver, E. H., Gupta, K., Francis, O., Kavanagh Williamson, M., Di Bartolo, N. D., Randell, M. J., Ben Khoud, Y., Kelland, I., Mortimer, G. L. M., Ball, O. E., Plumptre, C., Chandler, M. A., Obst, U., Secchi, M., Piemonti, L., & 27 othersLampasona, V., Smith, J., Gregorova, M., Knezevic, L., Metz, J., Barr, R. S., Morales-Aza, M. B., Oliver, J. L., Collingwood, L., Hitchings, B. E., Ring, S. M., Wooldridge, L., Rivino, L., Timpson, N. J., McKernon, J., Muir, P., Hamilton, F. W., Arnold, D. T., Woolfson, D. N., Goenka, A., Davidson, A. D., Toye, A. M., Berger, I., Bailey, M., Gillespie, K. M., Williams, A. J. K. & Finn, A., 9 Nov 2022, In: Frontiers in Immunology. 13, 20 p., 968317.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

2 Citations (Scopus)

88 Downloads (Pure)

Evaluation of isotype specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults

Thomas, A. C., Oliver, E., Baum, H. E., Gupta, K., Shelley, K. L., Long, A. E., Jones, H. E., Smith, J., Hitchings, B., di Bartolo, N., Vasileiou, K., Rabi, F., Alamir, H., Eghleilib, M., Francis, O., Oliver, J., Morales-Aza, B., Obst, U., Shattock, D., Barr, R., & 20 othersCollingwood, L., Duale, K., Grace, N., Livera, G. G., Bishop, L., Downing, H., Rodrigues, F., Timpson, N., Relton, C. L., Toye, A., Woolfson, D. N., Berger, I., Goenka, A., Davidson, A. D., Gillespie, K. M., Williams, A. J. K., Bailey, M., Brooks-Pollock, E., Finn, A. & Halliday, A., 2022

Research output: Other contribution

From peptides to proteins: coiled-coil tetramers to single-chain 4-helix bundles

Naudin, E. A., Albanese, K. I., Smith, A. J., Mylemans, B., Baker, E. G., Savery, N. J., Woolfson, D. N. & et, A., 20 Sept 2022, In: Chemical Science. 13, 38, p. 11330-11340 11 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

7 Citations (Scopus)

46 Downloads (Pure)

Generalized Born Implicit Solvent Models Do Not Reproduce Secondary Structures of De Novo Designed Glu/Lys Peptides

Lang, E. J. M., Baker, E. G., Woolfson, D. N. & Mulholland, A. J., 10 Jun 2022, (E-pub ahead of print) In: Journal of Chemical Theory and Computation. 18, 7, p. 4070−4076 7 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

14 Citations (Scopus)

80 Downloads (Pure)

Molecular architecture of the autoinhibited kinesin-1 lambda particle

Weijman, J. F. M. S., Yadav Kadapalakere, S., Surridge, K. J., Cross, J. A. A., Mantell, J. M., Woolfson, D. N., Berger-Schaffitzel, C. H. & Dodding, M. P., 16 Sept 2022, In: Science Advances.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

7 Citations (Scopus)

2021

A Brief History of De Novo Protein Design: Minimal, Rational, and Computational

Woolfson, D. N., 1 Oct 2021, In: Journal of Molecular Biology. 433, 20, 167160.

Research output: Contribution to journal › Review article (Academic Journal) › peer-review

Open Access

File

81 Citations (Scopus)

1815 Downloads (Pure)

Coiled coils 9-to-5: rational de novo design of α-helical barrels with tunable oligomeric states

Dawson, W. M., Martin, F. J. O., Rhys, G. G., Shelley, K. L., Brady, R. L. & Woolfson, D. N., 26 May 2021, In: Chemical Science. 12, 20, p. 6923-6928 6 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

28 Citations (Scopus)

62 Downloads (Pure)

Constructing ion channels from water-soluble α-helical barrels

Scott, A. J., Niitsu, A., Lang, E. J. M., Dawson, W. M., Brady, R. L., Mulholland, A. J. & Woolfson, D. N., 10 May 2021, In: Nature Chemistry. 13, 7, p. 643-650 8 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

61 Citations (Scopus)

113 Downloads (Pure)

Constructing synthetic-protein assemblies from de novo designed 310 helices

Kumar, P., Paterson, N. G., Clayden, J. & Woolfson, D. N., 11 Dec 2021, (Unpublished)

Research output: Other contribution

De Novo Designed Peptide and Protein Hairpins Self-assemble into Sheets and Nanoparticles

Galloway, J. M., Bray, H. E. V., Shoemark, D. K., Hodgson, L. R., Coombs, J., Mantell, J. M., Rose, R. S., Ross, J. F., Morris, C., Harniman, R. L., Wood, C. W., Arthur, C., Verkade, P. & Woolfson, D. N., 11 Mar 2021, In: Small. 17, 10, 11 p., 2100472.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

14 Citations (Scopus)

91 Downloads (Pure)

De novo design of a reversible phosphorylation-dependent switch for membrane targeting

Fletcher, J. M., Woolfson, D. N. & Schwille, P., 5 Mar 2021, In: Nature Communications. 12, 1, 1472.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

22 Citations (Scopus)

61 Downloads (Pure)

Fragment-linking peptide design yields a high-affinity ligand for microtubule-based transport

Cross, J. A., Chegkazi, M. S., Steiner, R. A., Woolfson, D. N. & Dodding, M. P., 16 Sept 2021, In: Cell Chemical Biology. 28, 9, p. 1347-1355.e5 15 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

6 Citations (Scopus)

78 Downloads (Pure)

How Coiled-Coil Assemblies Accommodate Multiple Aromatic Residues

Rhys, G. G., Dawson, W. M., Beesley, J. L., Martin, F. J. O., Brady, R. L., Thomson, A. R. & Woolfson, D. N., 21 Apr 2021, In: Biomacromolecules. 22, 5, p. 2010-2019 10 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

6 Citations (Scopus)

68 Downloads (Pure)

Implicit solvent models fail to reproduce secondary structures of de novo designed peptides

Lang, E. J. M., Baker, E. G., Woolfson, D. N. & Mulholland, A. J., 15 Nov 2021, (E-pub ahead of print) ChemRxiv, 16 p. (ChemRxiv).

Research output: Working paper › Preprint

File

46 Downloads (Pure)

Kinesin-1 captures RNA cargo in its adaptable coils

Cross, J. A. A., Woolfson, D. N. & Dodding, M. P., 1 Jul 2021, In: Genes and Development. 35, 13-14, p. 937-939 3 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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7 Citations (Scopus)

49 Downloads (Pure)

Molecular mechanism for kinesin-1 direct membrane recognition

Antón, Z., Weijman, J. F., Williams, C., Moody, E. R. R., Mantell, J., Yip, Y. Y., Cross, J. A., Williams, T. A., Steiner, R. A., Crump, M., Woolfson, D. N. & Dodding, M. P., 28 Jul 2021, In: Science Advances. 7, 31, 15 p., eabg6636.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

4 Citations (Scopus)

163 Downloads (Pure)

Query-Guided Protein-Protein Interaction Inhibitor Discovery

Bartlett, G. J., Avila Ibarra, A., Shoemark, D. K., Woolfson, D. N., Sessions, R. B., Andrews, D., Nelson, A. & Wilson, A. J., 7 Apr 2021, In: Chemical Science. 12, 13, p. 4753-4762 10 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

6 Citations (Scopus)

73 Downloads (Pure)

Scalable synthesis and coupling of quaternary α-arylated amino acids: α-aryl substituents are tolerated in α-helical peptides

Leonard, D. J., Zieleniewski, F., Wellhöfer, I., Baker, E. G., Ward, J. W., Woolfson, D. N. & Clayden, J., 21 Jul 2021, In: Chemical Science. 12, 27, p. 9386-9390 5 p., 9386.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

4 Citations (Scopus)

94 Downloads (Pure)

Socket2: A program for locating, visualizing and analyzing coiled-coil interfaces in protein structures

Kumar, P. & Woolfson, D. N., 1 Dec 2021, In: Bioinformatics. 37, 23, p. 4575-4577 3 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

30 Citations (Scopus)

127 Downloads (Pure)

Structural resolution of switchable states of a de novo peptide assembly

Dawson, W. M., Lang, E. J. M., Rhys, G. G., Shelley, K. L., Williams, C., Brady, R. L., Crump, M. P., Mulholland, A. J. & Woolfson, D. N., 9 Mar 2021, In: Nature Communications. 12, 1, 10 p., 1530 (2020).

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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16 Citations (Scopus)

121 Downloads (Pure)

Towards optimizing peptide-based inhibitors of protein-protein interactions: predictive saturation variation scanning (PreSaVS)

Hetherington, K., Dutt, S., Ibarra, A. A., Cawood, E. E., Hobor, F., Woolfson, D. N., Edwards, T. A., Nelson, A., Sessions, R. B. & Wilson, A. J., 7 Oct 2021, In: RSC chemical biology. 2, 5, p. 1474-1478 5 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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3 Citations (Scopus)

73 Downloads (Pure)

α-Helical peptides on plasma-treated polymers promote ciliation of airway epithelial cells

Mehrban, N., Scott, D. A., Woolfson, D. N., Callaghan, C. & et al., 1 Mar 2021, In: Materials Science and Engineering C. 122, p. 111935 1 p., 111935.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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2 Citations (Scopus)

45 Downloads (Pure)

2020

Automated solid-phase concatenation of Aib residues to form long, water-soluble, helical peptides

Zieleniewski, F. M., Woolfson, D. N. & Clayden, J. P., 14 Oct 2020, In: Chemical Communications. 56, 80, p. 12049-12052 4 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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11 Citations (Scopus)

123 Downloads (Pure)

BAlaS: fast, interactive and accessible computational alanine-scanning using BudeAlaScan

Wood, C. W., Avila Ibarra, A., Bartlett, G. J., Woolfson, D. N. & Sessions, R. B., 13 Jan 2020, (E-pub ahead of print) In: Bioinformatics. 3 p., btaa026.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

File

35 Citations (Scopus)

68 Downloads (Pure)

De Novo Designed Protein-Interaction Modules for In-Cell Applications

Edgell, C. L., Smith, A. J., Beesley, J. L., Savery, N. J. & Woolfson, D. N., 6 Feb 2020, In: ACS Synthetic Biology. 9, p. 427-436 10 p., 2.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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16 Citations (Scopus)

180 Downloads (Pure)

Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation

Juodeikis, R., Lee, M. J., Mayer, M., Mantell, J., Brown, I. R., Verkade, P., Woolfson, D. N., Prentice, M. B., Frank, S. & Warren, M. J., 13 Feb 2020, In: MicrobiologyOpen. 21 p., e1010.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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10 Citations (Scopus)

132 Downloads (Pure)

Host Macrophage Response to Injectable Hydrogels Derived From ECM and α-Helical Peptides

Mehrban, N., Molina, C. P., Quijano, L. M., Bowen, J., Johnson, S. A., Bartolacci, J., Chang, J., Scott, D. J., Woolfson, D. N., Birchall, M. & Badvlak, S., 15 Jul 2020, In: Acta Biomaterialia. 111, p. 12 141 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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25 Citations (Scopus)

128 Downloads (Pure)

Robust de novo designed homotetrameric coiled coils

Edgell, C. L., Savery, N. J. & Woolfson, D. N., 9 Mar 2020, (E-pub ahead of print) In: Biochemistry. 6 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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9 Citations (Scopus)

188 Downloads (Pure)

2019

A Modular Vaccine Platform Combining Self‐Assembled Peptide Cages and Immunogenic Peptides

Morris, C., Glennie, S., Lam, H., Baum, H., Kandage, D., Williams, N., Morgan, D., Woolfson, D. & Davidson, A., 21 Feb 2019, In: Advanced Functional Materials. 29, 8, 12 p., 1807357.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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36 Citations (Scopus)

233 Downloads (Pure)

Guiding Biomolecular Interactions in Cells Using de Novo Protein - Protein Interfaces

Smith, A., Thomas, F., Shoemark, D., Woolfson, D. & Savery, N., 21 Jun 2019, In: ACS Synthetic Biology. 8, 6, p. 1284-1293 10 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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28 Citations (Scopus)

174 Downloads (Pure)

Navigating the Structural Landscape of De Novo α-Helical Bundles

Rhys, G. G., Wood, C. W., Beesley, J. L., Zaccai, N. R., Burton, A. J., Brady, R. L., Thomson, A. R. & Woolfson, D. N., 5 Jun 2019, In: Journal of the American Chemical Society. 141, 22, p. 8787-8797 11 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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35 Citations (Scopus)

138 Downloads (Pure)

Peptide Assembly Directed and Quantified Using Megadalton DNA Nanostructures

Jin, J., Baker, E., Wood, C., Bath, J., Woolfson, D. & Tuberfield, A., 8 Aug 2019, (E-pub ahead of print) In: ACS Nano. 9 p.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access

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42 Citations (Scopus)

301 Downloads (Pure)

Professor Dek N Woolfson

Research output

Search results