Professor Jan Frayne

B.Sc.(Nott.), Ph.D.(Bristol)

  • BS8 1TD

1992 …2020

Research output per year

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Research Output

  • 62 Article (Academic Journal)
  • 8 Conference Contribution (Conference Proceeding)
  • 1 Chapter in a book
2020

Expression and retention of thymidine phosphorylase in cultured reticulocytes as a novel treatment for MNGIE

Meinders, M., Shoemark, D. K., Dobbe, J. G. G., Streekstra, G. J., Frayne, J. & Toye, A. M., 12 Jun 2020, In : Molecular Therapy - Methods and Clinical Development. 17, p. 822-830 9 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
19 Downloads (Pure)
2019

Comparing the two leading erythroid lines BEL-A and HUDEP-2

Daniels, D. E., Downes, D., Ferrer Vicens, I., Ferguson, D. C. J., Singleton, B. K., Wilson, M. C., Trakarnsanga, K., Kurita, R., Nakamura, Y., Anstee, D. J. & Frayne, J., 21 Nov 2019, In : Haematologica. 105, 3, 11 p., 229211.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
172 Downloads (Pure)

Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

Satchwell, T. J., Wright, K. E., Haydn-Smith, K. L., Sánchez-Román Terán, F., Moura, P. L., Hawksworth, J., Frayne, J., Toye, A. M. & Baum, J., 23 Aug 2019, In : Nature Communications. 10, 9 p., 3806 (2019).

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
114 Downloads (Pure)

Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation

Trakarnsanga, T., Ferguson, D., Daniels, D., Griffiths, R., Wilson, M., Mordue, K., Andrienko, T., Condie, A., McCahill, A., Mountford, J. C., Toye, A., Anstee, D. & Frayne, J., 29 Apr 2019, In : Stem Cell Research and Therapy. 10, 10 p., 130.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
1 Citation (Scopus)
152 Downloads (Pure)
2018

Enhancement of red blood cell transfusion compatibility using CRISPR‐mediated erythroblast gene editing

Hawksworth, J., Satchwell, T., Meinders, M., Daniels, D., Regan, F., Thornton, N., Wilson, M., Dobbe, J. G. G., Streekstra, G. J., Trakarnsanga, K., Heesom, K., Anstee, D., Frayne, J. & Toye, A., 26 Apr 2018, In : EMBO Molecular Medicine. 10, 5, 11 p., e8454.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
12 Citations (Scopus)
242 Downloads (Pure)

Optimization of an Erythroid Culture System to Reduce the Cost of in vitro Production of Red Blood Cells

Poldee, S., Metheetrairuta, C., Nugoolsuksiri, S., Frayne, J. & Trakarnsanga, T., 2018, In : MethodsX. 5, p. 1626-1632 7 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
2 Citations (Scopus)
210 Downloads (Pure)

Secretory factors from OP9 stromal cells delay differentiation and increase the expansion potential of adult erythroid cells in vitro

Trakarnsanga, K., Wilson, M. C., Heesom, K. J., Andrienko, T. N., Srisawat, C. & Frayne, J., 31 Jan 2018, In : Scientific Reports. 8, 1, 8 p., 1983.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
3 Citations (Scopus)
206 Downloads (Pure)
2017

Activation of KLF1 enhances the differentiation and maturation of red blood cells from human pluripotent stem cells

Yang, C-T., Ma, R., Frayne, J. & Forrester, LM., Apr 2017, In : Stem Cells. 35, 4, p. 886–897 12 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
18 Citations (Scopus)
392 Downloads (Pure)

An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

Trakarnsanga, K., Griffiths, R. E., Wilson, M. C., Blair, A., Satchwell, T. J., Meinders, M., Cogan, N., Kupzig, S., Kurita, R., Nakamura, Y., Toye, A. M., Anstee, D. J. & Frayne, J., 14 Mar 2017, In : Nature Communications. 8, 7 p., 14750.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
49 Citations (Scopus)
567 Downloads (Pure)
2016

Comparison of the proteome of adult and cord erythroid cells, and changes in the proteome following reticulocyte maturation

Wilson, M., Trakarnsanga, K., Heesom, K., Toye, A. & Frayne, J., 1 Jun 2016, In : Molecular and Cellular Proteomics. 15, 6, p. 1938-1946 9 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
48 Citations (Scopus)
330 Downloads (Pure)

Krüppeling erythropoiesis: an unexpected broad spectrum of human red blood cell disorders due to KLF1 variants

Perkins, A., Xu, X., Higgs, D., Patrinos, G. P., Arnaud, L., Bieker, J. J., Philipsen, S. & KLF1 Consensus Workgroup, 14 Apr 2016, In : Blood. 127, 15, p. 1856-1862 7 p.

Research output: Contribution to journalArticle (Academic Journal)

2014

Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL

Trakarnsanga, K., Wilson, M. C., Lau, W. W. Y., Singleton, B. K., Parsons, S. F., Sakuntanaga, P., Kurita, R., Nakamura, Y., Anstee, D. J. & Frayne, J., 2014, In : Haematologica. Aug 8. pii: haematol.2014.110155.

Research output: Contribution to journalArticle (Academic Journal)

27 Citations (Scopus)

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

Trakarnsanga, K., Wilson, M. C., Griffiths, R. E., Toye, A. M., Carpenter, L., Heesom, K. J., Parsons, S. F., Anstee, D. J. & Frayne, J., 2014, In : PLoS ONE. 9, 7, p. e100874

Research output: Contribution to journalArticle (Academic Journal)

24 Citations (Scopus)
2013

Epididymal specific, selenium-independent GPX5 protects cells from oxidative stress-induced lipid peroxidation and DNA mutation

Taylor, A., Robson, A., Houghton, B. C., Jepson, C. A., Ford, W. C. L. & Frayne, J., Sep 2013, In : Human Reproduction. 28, 9, p. 2332-2342 11 p.

Research output: Contribution to journalArticle (Academic Journal)

24 Citations (Scopus)
2012

Blood group phenotypes resulting from mutations in erythroid transcription factors.

Singleton, B. K., Frayne, J. & Anstee, D. J., Nov 2012, In : Current Opinion in Hematology. 19, 6, p. 486-493 8 p.

Research output: Contribution to journalArticle (Academic Journal)

17 Citations (Scopus)
2011

Mutations in the second zinc finger of human EKLF reduce promoter affinity but give rise to benign and disease phenotypes

Singleton, BK., Lau, W., Fairweather, VS., Burton, NM., Wilson, MC., Parsons, SF., Richardson, BM., Trakarnsanga, K., Brady, RL., Anstee, DJ. & Frayne, J., Jul 2011, In : Blood. 118, p. 3137 - 3145 9 p.

Research output: Contribution to journalArticle (Academic Journal)

34 Citations (Scopus)

Structure and kinetic characterization of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, GAPDS

Chaikuad, A., Shafqat, N., Al-Mokhtar, R., Cameron, G., Clarke, AR., Brady, RL., Oppermann, U., Frayne, J. & Yue, WW., Apr 2011, In : Biochemical Journal. 435 (2), p. 401 - 409 11 p.

Research output: Contribution to journalArticle (Academic Journal)

20 Citations (Scopus)
2009

Analysis of the differential proteome of human erythroblasts during in vitro erythropoiesis by 2-D DIGE

Richardson, BM., Heesom, KJ., Parsons, SF., Anstee, DJ. & Frayne, J., Sep 2009, In : Proteomics - Clinical applications. 3 (9), p. 1123 - 1134 12 p.

Research output: Contribution to journalArticle (Academic Journal)

5 Citations (Scopus)

A Novel EKLF Mutation in a Patient with Dyserythropoietic Anemia: The First Association of EKLF with Disease in Man.

Singleton, B. K., Fairweather, V. S. S., Lau, W., Parsons, S. F., Burton, N. M., Frayne, J., Brady, R. L. & Anstee, D. J., 20 Nov 2009, In : Blood. 114, 22, p. 72-72 1 p.

Research output: Contribution to journalArticle (Academic Journal)

25 Citations (Scopus)
2005

Spermatozoa. The spermatozoon at fertilisation: Current understanding and future research directions*

Brewis, IA., van Gestel, RA., Gadella, BM., Jones, R., Publicover, SJ., Roldan, ERS., Frayne, J. & Barratt, CLR., Dec 2005, In : Human Fertility. 8(4), p. 241 - 251 11 p.

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)
2002
39 Citations (Scopus)

Fertilin

Hall, L. & Frayne, J., 2002, Wiley Encyclopedia of Molecular Medicine. Creighton, TE. (ed.). John Wiley & Sons, Inc, p. 1265 - 1268

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Human tMDC III: a sperm protein with a potential role in oocyte recognition

Frayne, J., Hurd, EAC. & Hall, L., Sep 2002, In : Molecular Human Reproduction. 8 (9), p. 817 - 822 6 p.

Research output: Contribution to journalArticle (Academic Journal)

13 Citations (Scopus)
2001
22 Citations (Scopus)
1999

Effect of synthetic peptides on sperm binding and sperm-egg fusion in the mouse: identity of the putative integrin-binding motif

McLaughlin, EA., Frayne, J., Bloomberg, G. & Hall, L., 1999, In : Journal of Reproduction and Fertility Abstract Series. Abstract Series No 24, p. 8 - 9

Research output: Contribution to journalArticle (Academic Journal)

Localisation of phosphatidylethanolamine-binding protein in the brain and other tissues of the rat

Frayne, J., Ingram, CD., Love, S. & Hall, L., 1999, In : Cell and Tissue Research. 298, p. 415 - 423

Research output: Contribution to journalArticle (Academic Journal)

52 Citations (Scopus)

Mammalian sperm-egg recognition: does fertilin β have a major role to play?

Frayne, J. & Hall, L., 1999, In : BioEssays. 21, p. 183 - 187

Research output: Contribution to journalArticle (Academic Journal)

17 Citations (Scopus)

Non-functional fertility genes in humans: contributory factors in reduced male fertility?

Hall, L. & Frayne, J., 1999, In : Human Fertility. 2, p. 36 - 41

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)

The potential use of sperm antigens as targets for immunocontraception; past, present and future

Hall, L. & Frayne, J., 1999, In : Journal of Reproductive Immunology. 43, p. 1 - 33

Research output: Contribution to journalArticle (Academic Journal)

44 Citations (Scopus)

Transcripts encoding the sperm surface protein tMDC II are non-functional in the human

Frayne, J., Dimsey, EAC., Jury, JA. & Hall, L., 1999, In : Biochemical Journal. 341, p. 771 - 775

Research output: Contribution to journalArticle (Academic Journal)

17 Citations (Scopus)
1998

Expression of extracellular glutathione peroxidase type 5(GPX5) in the rat male reproductive tract

Williams, KM., Frayne, J. & Hall, L., 1998, In : Molecular Human Reproduction. 4, p. 841 - 848

Research output: Contribution to journalArticle (Academic Journal)

46 Citations (Scopus)

Expression of extracellular superoxide dismutase in the human male reproductive tract, detected using antisera raised against a recombinant protein

Williams, KM., Frayne, J., McLaughlin, EA. & Hall, L., 1998, In : Molecular Human Reproduction. 4, p. 235 - 242

Research output: Contribution to journalArticle (Academic Journal)

27 Citations (Scopus)

Expression of human epididymal glutathione peroxidase in the male reproductive tract.

K, W., Frayne, J. & Hall, L., Feb 1998, In : Boden Conference, Sydney, Australia, February 1998.

Research output: Contribution to journalArticle (Academic Journal)

71 Citations (Scopus)

Macaque MDC family of proteins: sequence analysis, tissue distribution and proteolytic processing in the male reproductive tract

Frayne, J., Jury, JA., Barker, HL., Perry, ACF., Jones, R. & Hall, L., 1998, In : Molec. Human Reprod. 4, p. 429 - 437

Research output: Contribution to journalArticle (Academic Journal)

26 Citations (Scopus)

Processing of macaque MDC proteins during epididymal transit

Frayne, J., Jury, JA., Barker, HL. & Hall, L., 1998, In : Boden Conference, Sydney, Australia, February 1998.

Research output: Contribution to journalArticle (Academic Journal)

Sequence analysis and characterisation of the rat orthologue of the TNF a converting enzyme (TACE)

Beaumont, AJ., Jury, JA., Frayne, J., Devos, R., Van Der Hayden, J., Jeffrey, L., Price, GJ. & Hall, L., 1998, Biochem Society 667th Annual Meeting, Leicester.

Research output: Chapter in Book/Report/Conference proceedingConference Contribution (Conference Proceeding)

42 Citations (Scopus)
42 Citations (Scopus)

The majority of human glutathione peroxidase type 5 (GPX5) transcripts are incorrectly spliced: implications for the role of GPX5 in the male reproductive tract

Hall, L., Williams, K., Perry, ACF., Frayne, J. & Jury, JA., 1998, In : Biochemical Journal. 333, p. 5 - 9

Research output: Contribution to journalArticle (Academic Journal)

61 Citations (Scopus)

The MDC family of proteins and their processing during epididymal transit

Frayne, J., Jury, JA., Barker, H. & Hall, L., 1998, In : Journal of Reproduction and Fertility. Suppl 53, p. 149 - 155

Research output: Contribution to journalArticle (Academic Journal)

19 Citations (Scopus)
1997

Cloning, sequencing and characterisation of rat fertilinβ

McLaughlin, EA., Barker, HL., Frayne, J., Ford, WCL., Jones, R. & Hall, L., 1997, In : Journal of Reproduction and Fertility Abstract Series. 19, p. 17 - 17 1 p.

Research output: Contribution to journalArticle (Academic Journal)

Cloning, sequencing and characterisation of rat fertilin β

McLaughlin, EA., Barker, HL., Frayne, J., Ford, WCL., Jones, R. & Hall, L., 1997, Unknown. Vol. 19. p. 28 - 28 1 p.

Research output: Chapter in Book/Report/Conference proceedingConference Contribution (Conference Proceeding)

Cloning and sequence analysis of rat fertilin α and β-developmental expression, procesing and immunolocalisation

McLaughlin, EA., Frayne, J., Barker, HL., Jury, JA., Jones, R., Ford, WCL. & Hall, L., 1997, In : Molec. Hum. Reprod. 3, p. 801 - 809

Research output: Contribution to journalArticle (Academic Journal)

Rat MDC family of proteins: Sequence analysis, tissue distribution and expression in prepubertal and adult rat testis

Frayne, J., Jury, JA., Barker, HL. & Hall, L., 1997, In : Molecular Reproduction and Development. 48, p. 159 - 167

Research output: Contribution to journalArticle (Academic Journal)

34 Citations (Scopus)