Dr Jim Spencer

B.A.(Cantab.), Ph.D.(Bristol)

  • BS8 1TD

19952020

Research output per year

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Research Output

  • 70 Article (Academic Journal)
  • 1 Conference Contribution (Conference Proceeding)
  • 1 Editorial (Academic Journal)
  • 1 Review article (Academic Journal)
2012

The Basis for Carbapenem Hydrolysis by Class A β-Lactamases: A Combined Investigation using Crystallography and Simulations.

Fonseca, M. F., Chudyk, E. I., Van der Kamp, M. W., Correia, A., Mulholland, A. J. & Spencer, J., 7 Nov 2012, In : Journal of the American Chemical Society. 134, 44, p. 18275-18285 11 p.

Research output: Contribution to journalArticle (Academic Journal)

45 Citations (Scopus)
2011

Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54

Fonseca, MF., Arthur, CJ., Bromley, EHC., Samyn, B., Moerman, P., Saavedra, MJ., Correia, A. & Spencer, J., Nov 2011, In : Antimicrobial Agents and Chemotherapy. 55, p. 5392 - 5395 4 p.

Research output: Contribution to journalArticle (Academic Journal)

9 Citations (Scopus)

Crystal structure of Serratia fonticola Sfh-I: activation of the nucleophile in mono-zinc metallo-β-lactamases

Fonseca, MF., Bromley, EHC., Saavedra, MJ., Correia, A. & Spencer, J., Jul 2011, In : Journal of Molecular Biology. 411, p. 951 - 959 9 p.

Research output: Contribution to journalArticle (Academic Journal)

32 Citations (Scopus)

Structural and computational investigations of VIM-7: insights into the substrate specificity of vim metallo-β-lactamases

Borra, PS., Leiros, HK., Ahmad, R., Spencer, J., Leiros, I., Walsh, TR., Sundsfjord, A. & Samuelsen, O., Aug 2011, In : Journal of Molecular Biology. 411, p. 174 - 189 16 p.

Research output: Contribution to journalArticle (Academic Journal)

29 Citations (Scopus)

Structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a Qnr protein from a Gram-negative bacterium

Xiong, X., Bromley, EHC., Oelschlaeger, P., Woolfson, DN. & Spencer, J., May 2011, In : Nucleic Acids Research. 39, 9, p. 3917 - 3927 11 p.

Research output: Contribution to journalArticle (Academic Journal)

45 Citations (Scopus)
2010

Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases

Spencer, J., Murphy, LM., Conners, R., Sessions, RB. & Gamblin, SJ., Mar 2010, In : Journal of Molecular Biology. 396 (4), p. 908 - 923 16 p.

Research output: Contribution to journalArticle (Academic Journal)

28 Citations (Scopus)

High-level expression and reconstitution of active Cfr, a radical-SAM rRNA methyltransferase that confers resistance to ribosome-acting antibiotics

Booth, MPS., Challand, MR., Emery, DC., Roach, PL. & Spencer, J., Dec 2010, In : Protein Expression and Purification. 74, p. 204 - 210 7 p.

Research output: Contribution to journalArticle (Academic Journal)

12 Citations (Scopus)
2009

Approaches to the simultaneous inactivation of metallo- and serine-beta-lactamases

Ganta, SR., Perumal, S., Pagadala, SR., Samuelsen, O., Spencer, J., Pratt, RF. & Buynak, JD., Feb 2009, In : Bioorganic and Medicinal Chemistry Letters. 19, p. 1618 - 1622 5 p.

Research output: Contribution to journalArticle (Academic Journal)

22 Citations (Scopus)

RE coil: an antimicrobial peptide regulator

Ryadnov, MG., Mukamolova, GV., Hawrani, AS., Spencer, J. & Platt, R., 14 Dec 2009, In : Angewandte Chemie - International Edition. 48, 51, p. 9676 - 9679 4 p.

Research output: Contribution to journalArticle (Academic Journal)

13 Citations (Scopus)
2008

Kinetic characterization of VIM-7, a divergent member of the VIM metallo-beta-lactamase family

Samuelsen, O., Castanheira, M., Walsh, TR. & Spencer, J., Aug 2008, In : Antimicrobial Agents and Chemotherapy. 52, 8, p. 2905 - 2908 4 p.

Research output: Contribution to journalArticle (Academic Journal)

25 Citations (Scopus)
2007

Structural basis for the role of Asp-120 in metallo-beta-lactamases

Crisp, JC., Conners, R., Garrity, JD., Carenbauer, AL., Crowder, MW. & Spencer, J., Sep 2007, In : Biochemistry. 46 (37), p. 10664 - 10674 11 p.

Research output: Contribution to journalArticle (Academic Journal)

25 Citations (Scopus)
2006

A New Approach to the Inhibition of Metallo-beta-Lactamases

Spencer, J. & Walsh, TR., Feb 2006, In : Angewandte Chemie - International Edition. 45, p. 1022 - 1026 5 p.

Research output: Contribution to journalArticle (Academic Journal)

40 Citations (Scopus)

Crystal Structure of Pseudomonas aeruginosa SPM-1 Provides Insights into Variable Zinc Affinity of Metallo-β-lactamases

Murphy, TA., Catto, LE., Halford, SE., Hadfield, AT., Minor, W., Walsh, TR. & Spencer, J., Mar 2006, In : Journal of Molecular Biology. 357 (3), p. 890 - 903 14 p.

Research output: Contribution to journalArticle (Academic Journal)

70 Citations (Scopus)

Metallo-beta-lactamases: novel weaponry for antibiotic resistance in bacteria

Crowder, MW., Spencer, J. & Vila, AJ., Oct 2006, In : Accounts of Chemical Research. 39, p. 721 - 728

Research output: Contribution to journalArticle (Academic Journal)

274 Citations (Scopus)

Metallo-β-lactamasen: ein neuer Inhibitionsansatz

Spencer, J. & Walsh, TR., Feb 2006, In : Angewandte Chemie. 118, 7, p. 1038-1042 5 p.

Research output: Contribution to journalArticle (Academic Journal)

2005

Antibiotic recognition by binuclear metallo-β-lactamases revealed by X-ray crystallography

Spencer, J., Read, J., Sessions, RB., Howell, S., Blackburn, GM. & Gamblin, SJ., Oct 2005, In : Journal of the American Chemical Society. 127 (41), p. 14439 - 14444 6 p.

Research output: Contribution to journalArticle (Academic Journal)

100 Citations (Scopus)
2004

Over-Expression, Purification and Characterization of Metallo-beta-Lactamase ImiS from Aeromonas veronii bv. sobria

Crawford, PA., Sharma, N., Chandrasekar, S., Sigdel, T., Walsh, TR., Spencer, J. & Crowder, MW., Aug 2004, In : Protein Expression and Purification. 36, p. 272 - 279 8 p.

Research output: Contribution to journalArticle (Academic Journal)

36 Citations (Scopus)
2001

Alternative rate-determining steps in hydrolysis of β-lactams by Stenotrophomonas maltophilia L1 metallo-β-lactamases

Spencer, J., Clarke, AR. & Walsh, TR., 2001, 41st Interscience Conference on Antimicrobial Agents and Chemotherapy. p. 107 - 107 1 p.

Research output: Chapter in Book/Report/Conference proceedingConference Contribution (Conference Proceeding)

Novel mechanism of hydrolysis of therapeutic β-lactams by Stenotrophomonas maltophilia L1 metallo-β-lactamase

Spencer, J., Clarke, AR. & Walsh, TR., Sep 2001, In : Journal of Biological Chemistry. 276 (36), p. 33638 - 33644 7 p.

Research output: Contribution to journalArticle (Academic Journal)

85 Citations (Scopus)

Pre-steady-state kinetic studies of mono- and binuclear metallo-beta-lactamases

Spencer, J., Simm, AM., Higgins, CS., Clarke, AR. & Walsh, TR., Aug 2001, In : Journal of Inorganic Biochemistry. 86, p. 440 - 440 1 p.

Research output: Contribution to journalArticle (Academic Journal)

1997

Structure of a kinetic protein folding intermediate revealed by equilibrium amide exchange

Hosszu, LLP., Craven, CJ., Parker, MJ., Lorch, M., Spencer, J., Clarke, AR. & Waltho, JP., 1997, In : Nature Structural Biology. 4, p. 801 - 804

Research output: Contribution to journalArticle (Academic Journal)

1995

An integrated kinetic analysis of intermediates and transition stated is protein folding reactions

Parker, MJ., Spencer, J. & Clarke, AR., 1995, In : Journal of Molecular Biology. 253, p. 771 - 786

Research output: Contribution to journalArticle (Academic Journal)

Characterisation of the structural and energetic properties of the intermediates on the folding pathways of PGK from Bacillus stearothermophilus

Parker, MJ., Burston, SG., Jackson, GS., Spencer, J., Staniforth, RA. & Clarke, AR., 1995, In : Protein Engineering. 8, p. 22 - 22 1 p.

Research output: Contribution to journalArticle (Academic Journal)