Dr John Crosby

B.Sc.(Hull), Ph.D.(Edin.)

  • BS8 1TS

1993 …2019

Research output per year

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Research Output

  • 69 Article (Academic Journal)
  • 1 Conference Abstract
  • 1 Review article (Academic Journal)
2019

Control of beta-Branching in Kalimantacin Biosynthesis: Application of 13C NMR to Polyketide Programming

Walker, P., Williams, C., Weir, A., Wang, L., Crosby, J., Race, P., Simpson, T., Willis, C. & Crump, M., 2 Sep 2019, In : Angewandte Chemie. 131, 36, p. 12576-12580 5 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
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8 Downloads (Pure)
2018

An Oxetane-Based Polyketide Surrogate to Probe Substrate Binding in a Polyketide Synthase

Ellis, B. D., Milligan, J. C., White, A. R., Duong, V., Altman, P. X., Mohammed, L. Y., Crump, M. P., Crosby, J., Luo, R., Vanderwal, C. D. & Tsai, S. C., 18 Apr 2018, In : Journal of the American Chemical Society. 140, 15, p. 4961-4964 4 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
9 Citations (Scopus)
171 Downloads (Pure)

Oxidative Addition, Transmetalation, and Reductive Elimination at a 2,2′-Bipyridyl-Ligated Gold Center

Harper, M. J., Arthur, C. J., Crosby, J., Emmett, E. J., Falconer, R. L., Fensham-Smith, A. J., Gates, P. J., Leman, T., McGrady, J. E., Bower, J. F. & Russell, C. A., 28 Mar 2018, In : Journal of the American Chemical Society. 140, 12, p. 4440-4445 6 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
37 Citations (Scopus)
269 Downloads (Pure)

Structural and Functional Studies of the Daunorubicin Priming Ketosynthase DpsC

Jackson, D. R., Shakya, G., Patel, A. B., Mohammed, L. Y., Vasilakis, K., Wattana-Amorn, P., Valentic, T. R., Milligan, J. C., Crump, M. P., Crosby, J. & Tsai, S. C., 19 Jan 2018, In : ACS Chemical Biology. 13, 1, p. 141-151 11 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
7 Citations (Scopus)
229 Downloads (Pure)
2017

Reclassification of the specialized metabolite producer Pseudomonas mesoacidophila ATCC 31433 as a member of the Burkholderia cepacia complex

Loveridge, E. J., Jones, C., Bull, M. J., Moody, S. C., Kahl, M. W., Khan, Z., Neilson, L., Tomeva, M., Adams, S. E., Wood, A. C., Rodriguez-Martin, D., Pinel, I., Parkhill, J., Mahenthiralingam, E. & Crosby, J., 1 Jul 2017, In : Journal of Bacteriology. 199, 13, e00125-17.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
11 Citations (Scopus)
250 Downloads (Pure)
2016

Recognition of extended linear and cyclised polyketide mimics by a Type II acyl carrier protein

Dong, X., Bailey, C., Williams, C., Crosby, J., Simpson, T., Willis, C. & Crump, M., 1 Mar 2016, In : Chemical Science. 7, 3, p. 1779-1785 7 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
6 Citations (Scopus)
289 Downloads (Pure)
2014

Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp SANK 73390

Murphy, A. C., Gao, S-S., Han, L-C., Carobene, S., Fukuda, D., Song, Z., Hothersall, J., Cox, R. J., Crosby, J., Crump, M. P., Thomas, C. M., Willis, C. L. & Simpson, T. J., 1 Jan 2014, In : Chemical Science. 5, 1, p. 397-402 6 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
23 Citations (Scopus)
349 Downloads (Pure)
2013

A conserved motif flags acyl carrier proteins for β-branching in polyketide synthesis

Haines, A. S., Dong, X., Song, Z., Farmer, R., Williams, C., Hothersall, J., Ploskon, E., Wattana-amorn, P., Stephens, E. R., Yamada, E., Gurney, R., Takebayashi, Y., Masschelein, J., Cox, R. J., Lavigne, R., Willis, C. L., Simpson, T. J., Crosby, J., Winn, P. J., Thomas, C. M. & 1 others, Crump, M. P., Nov 2013, In : Nature Chemical Biology. 9, 11, p. 685-692 8 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
33 Citations (Scopus)
549 Downloads (Pure)

Identification of Differential Protein Binding Affinities in an Atropisomeric Pharmaceutical Compound by Noncovalent Mass Spectrometry, Equilibrium Dialysis, and Nuclear Magnetic Resonance

Maple, H. J., Garlish, R. A., Whitcombe, I., Hold, A., Prosser, C. E., Ford, D., Mackenzie, H., Crosby, J., Porter, J., Taylor, R. J., Crump, M. P. & Crump, M. P., 18 Jun 2013, In : Analytical Chemistry. 85, 12, p. 5958–5964 7 p.

Research output: Contribution to journalArticle (Academic Journal)

4 Citations (Scopus)

The Determinants of Activity and Specificity in Actinorhodin Type II Polyketide Ketoreductase

Javidpour, P., Bruegger, J., Srithahan, S., Korman, T. P., Crump, M. P., Crosby, J., Burkart, M. D. & Tsai, S-C., 12 Sep 2013, In : Chemistry & Biology. 20, 10, p. 1225-1234 10 p.

Research output: Contribution to journalArticle (Academic Journal)

Open Access
File
18 Citations (Scopus)
351 Downloads (Pure)
2012

Automated protein-ligand interaction screening by mass spectrometry

Maple, H. J., Garlish, RA., Rigau-Roca, L., Porter, JR., Whitcombe, I., Prosser, CE., Kennedy, J., Henry, AJ., Taylor, RJ., Crump, MP. & Crosby, J., Jan 2012, In : Journal of Medicinal Chemistry. 55, 2, p. 837-851

Research output: Contribution to journalArticle (Academic Journal)

46 Citations (Scopus)

Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets

Crosby, J., Treadwell, T., Hammerton, M., Vasilakis, K., Crump, M. P., Williams, D. S. & Mann, S., 2012, In : Chemical Communications. 48, 97, p. 11832-11834 3 p.

Research output: Contribution to journalArticle (Academic Journal)

49 Citations (Scopus)

The Determinants of Activity and Specificity in Actinorhodin Type II Polyketide Ketoreductase (ActKR)

Javidpour, P. & Crosby, J., 2012, In : Chemistry & Biology.

Research output: Contribution to journalArticle (Academic Journal)

The structural role of the carrier protein - active controller or passive carrier

Crosby, J. & Crump, M. P., 2012, In : Natural Product Reports. 29, 10, p. 1111-1137 27 p.

Research output: Contribution to journalReview article (Academic Journal)

95 Citations (Scopus)
2011

Analysis of Streptomyces coelicolor Phosphopantetheinyl Transferase, AcpS, Reveals the Basis for Relaxed Substrate Specificity

Dall'Aglio, P., Arthur, C., Williams, C., Vasilakis, K., Maple, H. J., Crosby, J., Crump, M. P. & Hadfield, A. T., 28 Jun 2011, In : Biochemistry. 50, 25, p. 5704-5717 14 p.

Research output: Contribution to journalArticle (Academic Journal)

12 Citations (Scopus)

A Natural Plasmid Uniquely Encodes Two Biosynthetic Pathways Creating a Potent Anti-MRSA Antibiotic

Fukuda, D., Haines, A. S., Song, Z., Murphy, A. C., Hothersall, J., Stephens, E. R., Gurney, R., Cox, R. J., Crosby, J., Willis, C. L., Simpson, T. J. & Thomas, C. M., 31 Mar 2011, In : PLoS ONE. 6, 3, p. - 9 p., e18031.

Research output: Contribution to journalArticle (Academic Journal)

40 Citations (Scopus)

Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production

Hothersall, J., Murphy, A. C., Iqbal, Z., Campbell, G., Stephens, E. R., Wu, J., Cooper, H., Atkinson, S., Williams, P., Crosby, J., Willis, C. L., Cox, R. J., Simpson, T. J. & Thomas, C. M., May 2011, In : Applied Microbiology and Biotechnology. 90, 3, p. 1017-1026 10 p.

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)
2010

Phosphopantetheinylation and Specificity of Acyl Carrier Proteins in the Mupirocin Biosynthetic Cluster

Shields, JA., Rahman, AS., Arthur, CJ., Crosby, J., Hothersall, J., Simpson, TJ. & Thomas, CM., 2010, In : ChemBioChem. 11, p. 148 - 255

Research output: Contribution to journalArticle (Academic Journal)

10 Citations (Scopus)

Recognition of intermediate functionality by acyl carrier protein over a complete cycle of fatty acid biosynthesis

Ploskon, E., Arthur, CJ., Kanari, AL., Wattana-amorn, P., Williams, C., Crosby, J., Simpson, TJ., Willis, CL. & Crump, MP., 2010, In : Chemistry & Biology. 17, p. 776 - 785

Research output: Contribution to journalArticle (Academic Journal)

31 Citations (Scopus)

Solution Structure of an Acyl Carrier Protein Domain from a Fungal Type I Polyketide Synthase

Wattana-Amorn, P., Williams, C., Płoskoń, E., Cox, RJ., Simpson, TJ., Crosby, J. & Crump, MP., Mar 2010, In : Biochemistry. 49, p. 2186 - 2193 8 p.

Research output: Contribution to journalArticle (Academic Journal)

26 Citations (Scopus)
2009

Preliminary kinetic analysis of acyl carrier protein-ketoacylsynthase interactions in the actinorhodin minimal polyketide synthase

Beltran-Alvarez, P., Arthur, C. J., Cox, R. J., Crosby, J., Crump, M. P. & Simpson, T. J., 2009, In : Molecular bioSystems. 5, 5, p. 511-518 8 p.

Research output: Contribution to journalArticle (Academic Journal)

5 Citations (Scopus)

Probing the interactions of early polyketide intermediates with the actinorhodin ACP from S. coelicolor A3(2)

Evans, SE., Williams, C., Arthur, CJ., Ploskon, E., Crosby, J., Cox, RJ., Willis, CL., Simpson, TJ. & Crump, MP., 2009, In : Journal of Molecular Biology. 389, p. 511 - 528

Research output: Contribution to journalArticle (Academic Journal)

41 Citations (Scopus)

Structure and malonyl CoA-ACP transacylase binding of Streptomyces coelicolor fatty acid synthase acyl carrier protein

Arthur, C. J., Williams, C., Pottage, K., Płoskoń, E., Findlow, S. C., Burston, S. G., Simpson, T. J., Crump, M. P. & Crosby, J., Aug 2009, In : ACS Chemical Biology. 4 , 8, p. 625 - 636 12 p.

Research output: Contribution to journalArticle (Academic Journal)

30 Citations (Scopus)
2008

Accumulation of Mupirocin H and Mupiric Acid During In vivo Mutational Analysis of the Mupirocin Gene Cluster Reveals Labile Points in the Biosynthetic Pathway: the "Leaky Hosepipe" Mechanism

O'Connell, Y., Shields, JA., Cox, RJ., Crosby, J., Hothersall, J., Simpson, TJ., Thomas, CM. & Willis, CL., 2008, In : ChemBioChem. 9, p. 1500 - 1508 9 p.

Research output: Contribution to journalArticle (Academic Journal)

A Mammalian Type I Fatty Acid Synthase Acyl Carrier Protein Domain Does Not Sequester Acyl Chains

Ploskon, EA., Arthur, CJ., Evans, SE., Williams, C., Crosby, J., Simpson, TJ. & Crump, MP., 2008, In : Journal of Biological Chemistry. 283 (1), p. 518 - 528 11 p.

Research output: Contribution to journalArticle (Academic Journal)

53 Citations (Scopus)

An ACP Structural Switch: Conformational Differences between the Apo and Holo Forms of the Actinorhodin Polyketide Synthase Acyl Carrier Protein

Evans, SE., Williams, C., Arthur, CJ., Burston, SG., Simpson, TJ., Crosby, J. & Crump, MP., Oct 2008, In : ChemBioChem. 9, p. 2424 - 2432 9 p.

Research output: Contribution to journalArticle (Academic Journal)

37 Citations (Scopus)

In vivo Mutational Analysis of the Mupirocin Gene Cluster Reveals Labile Points in the Biosynthetic Pathway: the 'Leaky Hosepipe' Mechanism

Wu, J., Hothersall, J., Mazzetti, C., O'Connell, Y., Shields, J. A., Rahman, A. S., Cox, R. J., Crosby, J., Simpson, T. J., Thomas, C. M. & Willis, C. L., Jun 2008, In : ChemBioChem. 9 , 9, p. 1500 - 1508 9 p.

Research output: Contribution to journalArticle (Academic Journal)

29 Citations (Scopus)

Proposed Arrangement of Proteins Forming a Bacterial Type II Polyketide Synthase

Castaldo, G., Zucko, J., Heidelberger, S., Vujaklija, D., Hranueli, D., Cullum, J., Wattana-Amorn, P., Crump, MP., Crosby, J. & Long, PF., 2008, In : Chemistry & Biology. 15, p. 1156 - 1165

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)
2007

BIOL 130-Mechanism of the assembly of the pseudomonic acids

Wu, JE., Hothersall, J., Cooper, S. M., Cox, R. J., Crosby, J., Thomas, C. M., Willis, C. L. & Simpson, T. J., 19 Aug 2007, p. -. 1 p.

Research output: Contribution to conferenceConference Abstract

Dissecting the Component Reactions Catalysed by the Actinorhodin Minimal Polyketide Synthase

Beltran-Alvarez, P., Cox, RJ., Crosby, J. & Simpson, TJ., Nov 2007, In : Biochemistry. 46(50), p. 14672 - 14681 9 p.

Research output: Contribution to journalArticle (Academic Journal)

27 Citations (Scopus)

Mupirocin H, a novel metabolite isolated from mutation of the HMG-GoA synthase analogue, mupH in Pseudomonas fluorescens

Wu, J., Cooper, SM., Cox, RJ., Crosby, J., Crump, MP., Hothersall, J., Simpson, TJ., Thomas, CM. & Willis, CL., Feb 2007, In : Chemical Communications. 20, p. 2040 - 2042 3 p.

Research output: Contribution to journalArticle (Academic Journal)

33 Citations (Scopus)

Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by Pseudomonas fluorescens: Pseudomic acid B biosynthesis precedes pseudomonic acid A

Hothersall, J., Wu, J., Rahman, AS., Shields, JA., Haddock, J., Johnson, N., Cooper, SM., Stephens, ER., Cox, RJ., Crosby, J., Willis, CL., Simpson, TJ. & Thomas, CM., May 2007, In : Journal of Biological Chemistry. 282, 21, p. 15451 - 15461 11 p.

Research output: Contribution to journalArticle (Academic Journal)

27 Citations (Scopus)

Structural Insights into the Interaction of Insulin-like Growth Factor 2 with IGF2R Domain 11

Williams, C., Rezgui, D., Prince, SN., Zaccheo, OJ., Foulstone, EJ., Forbes, BE., Norton, RS., Crosby, J., Hassan, AB. & Crump, MP., Sep 2007, In : Structure. 15 (9), p. 1065 - 1078 14 p.

Research output: Contribution to journalArticle (Academic Journal)

28 Citations (Scopus)
2006

The malonyl transferase activity of type II polyketide synthase acyl carrier proteins

Arthur, CJ., Szafranska, A., Long, J., Mills, J., Cox, RJ., Findlow, SC., Simpson, TJ., Crump, MP. & Crosby, J., 23 Jun 2006, In : Chemistry and Biology. 13 (6), p. 587 - 596 10 p.

Research output: Contribution to journalArticle (Academic Journal)

24 Citations (Scopus)
2005

Bulgecin A: a novel inhibitor of binuclear metallo-beta-lactamases

Simm, AM., Loveridge, EJ., Crosby, J., Avison, MB., Walsh, TR. & Bennett, PM., May 2005, In : Biochemical Journal. 387, p. 585 - 590 6 p.

Research output: Contribution to journalArticle (Academic Journal)

31 Citations (Scopus)

Exploring protein interactions on a minimal type II polyketide synthase using a yeast two-hybrid system

Castaldo, G., Crosby, J. & Long, PF., 28 Feb 2005, In : Food Technology & Biotechnology. 43(2), p. 109 - 112 4 p.

Research output: Contribution to journalArticle (Academic Journal)

3 Citations (Scopus)

Mupirocin W, a novel pseudomonic acid produced by targeted mutation of the mupirocin biosynthetic gene cluster

Cooper, SM., Cox, RJ., Crosby, J., Crump, MP., Hothersall, J., Laosripaiboon, W., Simpson, TJ. & Thomas, CM., Mar 2005, In : Chemical Communications. (9), p. 1179 - 1181 3 p.

Research output: Contribution to journalArticle (Academic Journal)

28 Citations (Scopus)

Self-malonylation is an intrinsic property of a chemically synthesized type II polyketide synthase acyl carrier protein

Arthur, CJ., Szafranska, A., Evans, SE., Findlow, SC., Burston, SG., Owen, P., Clark-Lewis, I., Simpson, TJ., Crosby, J. & Crump, MP., Nov 2005, In : Biochemistry. 44 (46), p. 15414 - 15421 8 p.

Research output: Contribution to journalArticle (Academic Journal)

30 Citations (Scopus)

Shift to Pseudomonic Acid B Production in Pseudomonas fluorescens NCIMB10586 by Mutation of Mupirocin Tailoring Genes mupO, mupU, mupV and macpE

Cooper, SM., Laosripaiboon, W., Rahman, AS., Hothersall, J., El-Sayed, AK., Winfield, CJ., Crosby, J., Cox, RJ., Simpson, TJ. & Thomas, CM., Jul 2005, In : Chem. Biol. 12(7), p. 825 - 833 8 p.

Research output: Contribution to journalArticle (Academic Journal)

22 Citations (Scopus)

Tandemly Duplicated Acyl Carrier Proteins Which Increase Polyketide Antibiotic Production can Apparently Function Either in Parallel or in Series

Simpson, TJ., Crosby, J., Thomas, CM., Rahman, AS. & Hothersall, J., 25 Feb 2005, In : Journal of Biological Chemistry. 280(8), p. 6399 - 6408 10 p.

Research output: Contribution to journalArticle (Academic Journal)

46 Citations (Scopus)
2003

Endogenous β-carbolines as clonidine displacing substances

Robinson, ESJ., Anderson, NJ., Crosby, J., Nutt, DJ. & Hudson, AL., 2003, In : Annals of the New York Academy of Sciences. 1099, p. 157 - 166 15 p.

Research output: Contribution to journalArticle (Academic Journal)

41 Citations (Scopus)

First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

Nicholson, TP., Winfield, CJ., Westcott, J., Crosby, J., Simpson, TJ. & Cox, RJ., Mar 2003, In : Chemical Communications. 6, p. 686 - 687 2 p.

Research output: Contribution to journalArticle (Academic Journal)

30 Citations (Scopus)

Solution structure and dynamics of Oxytetracycline polyketide synthase ACP from S. rimosus

Simpson, TJ., Crosby, J., Crump, MP., Findlow, IS. & Winsor, C., May 2003, In : Biochemistry. 42, p. 8423 - 8433 11 p.

Research output: Contribution to journalArticle (Academic Journal)

The isolated Acyl Carrier Protein Domain from the Type I Rat Fatty Acid Synthase Shows Considerable Homology and Analogous Biochemical Properties to Type II Acyl Carrier Proteins

Simpson, TJ., Crosby, J., Cox, RJ., Arthur, CJ., Nicholson, TP., Szafranska, AE., Reed, MAC., Crump, MP. & Schweizer, M., 2003, In : Organic and Biomolecular Chemistry. 1, p. 463 - 471

Research output: Contribution to journalArticle (Academic Journal)

2002

Kinetic and Mechanistic Analysis of the Malonyl CoA:ACP Transacylase from Streptomyces coelicolor Indicates a Single Catalytically Competent Serine Nucleophile at the Active Site

Szafranska, AE., Hitchman, TS., Cox, RJ., Crosby, J. & Simpson, TJ., Feb 2002, In : Biochemistry. 41 (5), p. 1421 - 1427 7 p.

Research output: Contribution to journalArticle (Academic Journal)

37 Citations (Scopus)

Streptomyces coelicolor phosphopantetheinyl transferase: a promiscuous activator of polyketide and fatty acid synthase acyl carrier proteins

Cox, RJ., Crosby, J., Daltrop, O., Glod, F., Jarzabek, ME., Nicholson, TP., Reed, MAC., Simpson, TJ., Smith, LH., Soulas, F., Szafranska, AE. & Westcott, J., Jun 2002, In : Perkin 1. 14, p. 1644 - 1649 6 p.

Research output: Contribution to journalArticle (Academic Journal)

33 Citations (Scopus)

Synthesis and Characterisation of Acyl Carrier Protein Bound Polyketide Analogues

Winfield, CJ., Simpson, TJ., Crosby, J., Cox, RJ., Szafranska, AE., Westcott, J., Arthur, CJ., Rahman, M., Soulas, F. & Spogli, R., 2002, In : ChemBioChem. p. 253 - 257

Research output: Contribution to journalArticle (Academic Journal)

15 Citations (Scopus)
2001

High performance liquid chromatography/electrospray tandem mass spectrometry of polycyclic aromatic hydrocarbons

Crosby, J., Brereton, RG. & Airiau, C., Jan 2001, In : Rapid Communications in Mass Spectrometry. 15(2), p. 135 - 140 5 p.

Research output: Contribution to journalArticle (Academic Journal)

Solid Phase Synthesis of 4-Hydroxycinnamic Acid and its Derivatives for Potential Use in Combinatorial Chemistry: A Novel Route for the Synthesis of 4-Hydroxycinnamoyl Coenzyme A and NMDA Receptor Antagonists

Mcintyre, BG., Martinez Bermejo, F., Srivastava, SK., Husbands, SM., Simpson, TJ., Crosby, J. & Lewis, JW., 2001, In : Combinatorial Chemistry and High Throughput Screening. 4, p. 111 - 114

Research output: Contribution to journalArticle (Academic Journal)

1 Citation (Scopus)
1999

A chain initiation factor common to both modular and aromatic polyketide synthases

Bisang, C., Long, PF., Cortés, J., Westcott, J., Crosby, J., Matharu, A-L., Cox, RJ., Simpson, TJ., Staunton, J. & Leadlay, PF., 1999, In : Nature. 401, p. 502 - 505

Research output: Contribution to journalArticle (Academic Journal)

219 Citations (Scopus)