Projects per year
Personal profile
Research interests
My lab is interested in autophagy - the regulated recycling of cytoplasmic material through delivery and degradation in the lysosome.
Please visit the Lab website https://thelanelab.blogs.bristol.ac.uk/
Follow Jon on Twitter @Jon_D_Lane
Autophagy (macroautophagy) is characterised by the formation of double membrane-bound organelles that sequester regions of cytoplasm including misfolded protein aggregates and organelles.The autophagosome membrane is decorated with a protein known as Atg8 (commonly known as LC3), which plays roles in autophagosome assembly and cargo selection.
We use live-cell imaging as well as fixed cell microscopy (including electron microscopy) to explore how and where autophagosomes are assembled. Through the application of cell-lines expressing GFP-tagged autophagy proteins (such as Atg5; Atg14; Atg16L; DFCP1), we can determine how the sequential recruitment of autophagy factors influences autophagosome assembly.
In our studies we used various human and mouse cell culture lines, primary human erythroid precursors and induced pluripotent stem cells from human patients. The latter we differentiate into specific neural lineages to understand how autophagy is regulated in neurons for research into the causes of neurodegenerative diseases (Parkinson’s disease; Alzheimer’s disease).
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Projects
Remodelling the isolation membrane: roles of sorting nexins during autophagy
1/01/12 → 1/01/15
Project: Research
Research Output
A heterodimeric SNX4:SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly
Anton, Z., Betin, V. M. S., Simonetti, B., Traer, C. J., Attar, N., Cullen, P. J. & Lane, J. D., 15 Jul 2020, In : Journal of Cell Science. 133, 14, 38 p., jcs246306.Research output: Contribution to journal › Article (Academic Journal)
Open Access
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A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons
Stathakos, P., Jiménez Moreno, N., Crompton, L. A., Nistor, P. A., Badger, J. L., Barbuti, P. A., Kerrigan, T. L., Randall, A. D., Caldwell, M. A. & Lane, J. D., 14 Apr 2020, In : Autophagy. 17 p.Research output: Contribution to journal › Article (Academic Journal)
Open Access
File
Mitochondrial import, health and mtDNA copy number variability using Type II and Type V CRISPR effectors
Anton, Z., Mullally, G. E. L., Ford, H. C., Van Der Kamp, M. W., Szczelkun, M. D. & Lane, J. D., 10 Aug 2020, (Accepted/In press) In : Journal of Cell Science.Research output: Contribution to journal › Article (Academic Journal)
Supervised Work
Investigation into the Effect of Protein Disulphide Isomerase A3 on the Prom-Metastatic Phenotype of Breast Cancer Cells
Author: Young, H., 23 Jan 2019Supervisor: Lane, J. (Supervisor) & Adams, J. (Supervisor)
Student thesis: Master's Thesis › Master of Science (MSc)
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Proteomics-Based Analysis of The Coordination of Membrane Trafficking Events by The ATG12-ATG5 Autophagy Complex
Author: Baines, K., 23 Jan 2019Supervisor: Lane, J. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy (PhD)
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Reciprocal Autophagy Control by the LIM Homeodomain Transcription Factors LMX1A and LMX1B Safeguards Human Midbrain Dopaminergic Neurons
Author: Jimenez Moreno, N., 23 Jun 2020Supervisor: Lane, J. (Supervisor), Cullen, P. (Supervisor) & Martin, P. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy (PhD)
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