Projects per year
My lab is interested in autophagy - the regulated recycling of cytoplasmic material through delivery and degradation in the lysosome.
Please visit the Lab website https://thelanelab.blogs.bristol.ac.uk/
Follow Jon on Twitter @Jon_D_Lane
Autophagy (macroautophagy) is characterised by the formation of double membrane-bound organelles that sequester regions of cytoplasm including misfolded protein aggregates and organelles.The autophagosome membrane is decorated with a protein known as Atg8 (commonly known as LC3), which plays roles in autophagosome assembly and cargo selection.
We use live-cell imaging as well as fixed cell microscopy (including electron microscopy) to explore how and where autophagosomes are assembled. Through the application of cell-lines expressing GFP-tagged autophagy proteins (such as Atg5; Atg14; Atg16L; DFCP1), we can determine how the sequential recruitment of autophagy factors influences autophagosome assembly.
In our studies we used various human and mouse cell culture lines, primary human erythroid precursors and induced pluripotent stem cells from human patients. The latter we differentiate into specific neural lineages to understand how autophagy is regulated in neurons for research into the causes of neurodegenerative diseases (Parkinson’s disease; Alzheimer’s disease).
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13/10/20 → 12/10/23
1/10/20 → 30/09/23
1/07/14 → …
Project: Research, Parent
Efficient and Scalable Generation of Human Ventral Midbrain Astrocytes from Human-Induced Pluripotent Stem CellsCrompton, L. A., McComish, S. F., Stathakos, P., Cordero Llana, O., Lane, J. D. & Caldwell, M. A., 2 Oct 2021, In: Journal of Visualized Experiments.
Research output: Contribution to journal › Article (Academic Journal) › peer-review
A heterodimeric SNX4:SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assemblyAnton, Z., Betin, V. M. S., Simonetti, B., Traer, C. J., Attar, N., Cullen, P. J. & Lane, J. D., 15 Jul 2020, In: Journal of Cell Science. 133, 14, 38 p., jcs246306.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile30 Downloads (Pure)
Stathakos, P., Jiménez Moreno, N., Crompton, L. A., Nistor, P. A., Badger, J. L., Barbuti, P. A., Kerrigan, T. L., Randall, A. D., Caldwell, M. A. & Lane, J. D., 14 Apr 2020, In: Autophagy. 17 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile43 Downloads (Pure)