20012020

Research output per year

If you made any changes in Pure these will be visible here soon.

Personal profile

Research interests

My lab is interested in autophagy - the regulated recycling of cytoplasmic material through delivery and degradation in the lysosome. 

Please visit the Lab website https://thelanelab.blogs.bristol.ac.uk/ 

Follow Jon on Twitter @Jon_D_Lane

Autophagy (macroautophagy) is characterised by the formation of double membrane-bound organelles that sequester regions of cytoplasm including misfolded protein aggregates and organelles.The autophagosome membrane is decorated with a protein known as Atg8 (commonly known as LC3), which plays roles in autophagosome assembly and cargo selection.

We use live-cell imaging as well as fixed cell microscopy (including electron microscopy) to explore how and where autophagosomes are assembled. Through the application of cell-lines expressing GFP-tagged autophagy proteins (such as Atg5; Atg14; Atg16L; DFCP1), we can determine how the sequential recruitment of autophagy factors influences autophagosome assembly.

In our studies we used various human and mouse cell culture lines, primary human erythroid precursors and induced pluripotent stem cells from human patients. The latter we differentiate into specific neural lineages to understand how autophagy is regulated in neurons for research into the causes of neurodegenerative diseases (Parkinson’s disease; Alzheimer’s disease).

 

Fingerprint Dive into the research topics where Jon D Lane is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 1 Similar Profiles

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Projects

Research Output

A heterodimeric SNX4:SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly

Anton, Z., Betin, V. M. S., Simonetti, B., Traer, C. J., Attar, N., Cullen, P. J. & Lane, J. D., 2 Jun 2020, (Accepted/In press) In : Journal of Cell Science.

Research output: Contribution to journalArticle (Academic Journal)

  • A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons

    Stathakos, P., Jiménez Moreno, N., Crompton, L. A., Nistor, P. A., Badger, J. L., Barbuti, P. A., Kerrigan, T. L., Randall, A. D., Caldwell, M. A. & Lane, J. D., 23 Jan 2020, (Accepted/In press) In : Autophagy.

    Research output: Contribution to journalArticle (Academic Journal)

  • DNA damage signalling from the placenta to foetal blood as a potential mechanism for childhood leukaemia initiation

    Mansell, E., Zareian, N., Malouf, C., Kapeni, C., Brown, N., Badie, C., Baird, D., Lane, J. D., Ottersbach, K., Blair, A. & Case, C. P., 13 Mar 2019, In : Scientific Reports. 9, 1, 17 p., 4370.

    Research output: Contribution to journalArticle (Academic Journal)

    Open Access
    File
  • 2 Citations (Scopus)
    160 Downloads (Pure)

    Thesis

    Investigation into the Effect of Protein Disulphide Isomerase A3 on the Prom-Metastatic Phenotype of Breast Cancer Cells

    Author: Young, H., 23 Jan 2019

    Supervisor: Lane, J. (Supervisor) & Adams, J. (Supervisor)

    Student thesis: Master's ThesisMaster of Science (MSc)

    File

    Proteomics-Based Analysis of The Coordination of Membrane Trafficking Events by The ATG12-ATG5 Autophagy Complex

    Author: Baines, K., 23 Jan 2019

    Supervisor: Lane, J. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy (PhD)

    File

    The Engineering and Evaluation of Genome Editing Tools in Mitochondria

    Author: Mullally, G., 23 Jan 2019

    Supervisor: Szczelkun, M. (Supervisor) & Lane, J. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy (PhD)

    File