Projects per year
As a Research Fellow within the Neuro-Epigenetic Research Group I am interested in the regulation of signalling, epigenetic and genomic process which occur in the brain following stress. Our group is particularly interested in the function of the hippocampus under baseline conditions, and after stress, in regulating the HPA axis and facilitating adaptive responses towards stress resilience. Personally I am also very interested in understand how the extracellular matrix can influence adaptive learning processes.
In order to pursue these investigations we have developed novel advanced chromatin immunoprecipitation (ChIP) methods to understand protein-DNA interactions including sequential and tandem ChIP (Mifsud & Reul, PNAS, 2016). Other techniques used in our lab include immunohistochemistry, RNA extraction and analysis, quantitative PCR, RNAScope, western blotting, radioimmunoassays and ELISA.
Unexpected effects of metyrapone on corticosteroid receptor interaction with the genome and subsequent gene transcription in the hippocampus of male ratsKennedy, C. LM., Carter, S. D., Mifsud, K. R. & Reul, J. M. H. M., Feb 2020, In: Journal of Neuroendocrinology. 32, 2, 12 p., e12820.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile19 Downloads (Pure)
Mineralocorticoid and glucocorticoid receptor-mediated control of genomic responses to stress in the brainMifsud, K. R. & Reul, J. M. H. M., 4 Apr 2018, In: Stress. p. 1-14 14 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile14 Citations (Scopus)361 Downloads (Pure)
Acute stress enhances epigenetic modifications but does not affect the constitutive binding of pCREB to Immediate-early gene promoters in the rat hippocampusCarter, S., Mifsud, K. & Reul, H., 19 Dec 2017, In: Frontiers in Molecular Neuroscience. 10, 416.
Research output: Contribution to journal › Article (Academic Journal) › peer-reviewOpen AccessFile4 Citations (Scopus)295 Downloads (Pure)