Professor Mark S Dillingham

B.Sc.(Bristol), Ph.D.(Oxon.)

  • BS8 1TD

19962020

Research output per year

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Personal profile

Research interests

Helicases as modular components of DNA processing machines

Helicases are motor proteins that translocate along and unwind duplex nucleic acids into their component single strands in an ATP-dependent manner. They are exceptionally abundant enzymes and constitute about 1% of the proteome. Accordingly, they are involved in a wide variety of nucleic acid transactions including DNA replication, repair, recombination and transcription and virtually every aspect of RNA metabolism. Our research is focused on uncovering the role of such helicases in complex DNA manipulations, including the processing of broken DNA for repair by homologous recombination and the resolution of conflicts between DNA replication and transcription.

Figure: AddAB helicase-nuclease bound to a DNA break (Saikrishnan et al., EMBO J. 2012, 31, 1568)

 

Chromosome dynamics

Genetic information is commonly stored in very large DNA molecules called chromosomes. These molecules must be efficiently condensed and segregated into daughter cells within the confines of a crowded cell. Remarkably, a whole myriad of other DNA transactions including transcription and repair occur simultaneously on the same molecule. Using Bacillus subtilis as a model system, we are studying the co-ordination of DNA replication, condensation and segregation, with a particular emphasis on the role of structural maintenance of chromosomes (SMC) proteins.

Figure: V- and O-shaped SMC proteins imaged by atomic force microscopy (Fuentes-Perez et al. Biophys. J., 2012, 102, 839)

 

Join our group!

We are always interested in hearing from talented scientists who wish to join the laboratory. Please contact Mark Dillingham for an informal discussion of the opportunities that are currently available.

 

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Projects

Research Output

  • 64 Article (Academic Journal)

On the mechanism of recombination hotspot scanning during double-stranded DNA break resection

Carrasco, C., Gilhooly, N. S., Dillingham, M. S. & Moreno-Herrero, F., 24 Jun 2013, In : Proceedings of the National Academy of Sciences of the United States of America.

Research output: Contribution to journalArticle (Academic Journal)

  • 21 Citations (Scopus)

    Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex

    Saikrishnan, K., Yeeles, J. T., Gilhooly, N. S., Krajewski, W. W., Dillingham, M. S. & Wigley, D. B., Mar 2012, In : EMBO Journal. 31, 6, p. 1568 - 1578 11 p.

    Research output: Contribution to journalArticle (Academic Journal)

  • 40 Citations (Scopus)

    Recombination hotspots and single-stranded DNA binding proteins couple DNA translocation to DNA unwinding by the AddAB helicase-nuclease

    Yeeles, J., van Aelst, K., Dillingham, M. S. & Moreno-Herrero, F., Jun 2011, In : Molecular Cell. 42, 6, p. 806 - 816 11 p.

    Research output: Contribution to journalArticle (Academic Journal)

  • 28 Citations (Scopus)

    Prizes

    The Colworth Medal

    Dillingham, Mark S (Recipient), 2010

    Prize: Prizes, Medals, Awards and Grants

  • Activities

    • 3 Participation in conference
    • 3 Membership or peer review panel or committee
    • 3 Fellowship awarded competitively
    • 2 - Research and Teaching at External Organisation

    The 9th Nucleic Acids Forum

    Mark S Dillingham (Organiser)

    2013

    Activity: Participating in or organising an event typesParticipation in conference

    Machines on Genes II

    Mark S Dillingham (Organiser)

    2012

    Activity: Participating in or organising an event typesParticipation in conference