CHEMICAL ANALYSIS OF HYBRID FUNGAL MEGASYNTHASES

Search results

• 2018

  Strobilurin biosynthesis in Basidiomycete fungi

  Nofiani, R., de Mattos-Shipley, K., Lebe, K. E., Han, L. C., Iqbal, Z., Bailey, A. M., Willis, C. L., Simpson, T. J. & Cox, R. J., 26 Sept 2018, In: Nature Communications. 9, 11 p., 3940.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Anabolism 100%
  • Basidiomycete 100%
  • Gene Cluster 100%
  • Methyltransferase 100%
  • Coenzyme A 100%

  87 Citations (Scopus)

  430 Downloads (Pure)

• 2017

  Genetic and chemical characterisation of the cornexistin pathway provides further insight into maleidride biosynthesis

  Williams, K., Szwalbe, A. J., Dickson, C., Desson, T. R., Mulholland, N. P., Vincent, J. L., Clough, J. M., Bailey, A. M., Butts, C. P., Willis, C. L., Simpson, T. J. & Cox, R. J., 18 Jul 2017, In: Chemical Communications. 53, 56, p. 7965-7968 4 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Herbicide 100%
  • Dimerization 100%
  • Maleic Anhydride 100%
  • Biosynthesis 100%
  • Anabolism 100%

  17 Citations (Scopus)

  534 Downloads (Pure)

• In vitro kinetic study of the squalestatin tetraketide synthase dehydratase reveals the stereochemical course of a fungal highly reducing polyketide synthase

  Liddle, E., Scott, A., Han, L. C., Ivison, D., Simpson, T. J., Willis, C. L. & Cox, R. J., 4 Feb 2017, In: Chemical Communications. 53, 10, p. 1727-1730 4 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Hydrolyase 100%
  • Cysteamine 100%
  • Squalestatin 100%
  • Synthetase 100%
  • Polyketide Synthase 100%

  18 Citations (Scopus)

  392 Downloads (Pure)

• Selected Mutations Reveal New Intermediates in the Biosynthesis of Mupirocin and the Thiomarinol Antibiotics

  Gao, S., Wang, L., Song, Z., Hothersall, J., Stevens, E. R., Connolly, J., Winn, P. J., Cox, R., Crump, M., Race, P., Simpson, T. & Willis, C., 27 Mar 2017, In: Angewandte Chemie - International Edition. 56, 14, p. 3930-3934 5 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Tetrahydropyran 100%
  • Mupirocin 100%
  • Anabolism 100%
  • Antibiotics 100%
  • Polyketide 25%

  16 Citations (Scopus)

  418 Downloads (Pure)

• Substrate selectivity of an isolated enoyl reductase catalytic domain from an iterative highly reducing fungal polyketide synthase reveals key components of programming

  Roberts, D. M., Bartel, C., Scott, A., Ivison, D., Simpson, T. J. & Cox, R. J., 1 Feb 2017, In: Chemical Science. 8, 2, p. 1116-1126 11 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Reductase 100%
  • Oxidoreductase 100%
  • Polyketide Synthase 100%
  • Enzyme Active Site 100%
  • Thioester 22%

  26 Citations (Scopus)

  437 Downloads (Pure)

• 2016

  Heterologous Production of Fungal Maleidrides Reveals the Cryptic Cyclization Involved in their Biosynthesis

  Williams, K., Szwalbe, A. J., Mulholland, N. P., Vincent, J. L., Bailey, A., Willis, C. L., Simpson, T. J. & Cox, R. J., 1 Jun 2016, In: Angewandte Chemie - International Edition. 55, 23, p. 6784-6788 5 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Hydrolase 100%
  • Precursor 100%
  • Gene Targeting 100%
  • Heterologous Expression 100%
  • Gene Disruption 100%

  54 Citations (Scopus)

  809 Downloads (Pure)

• Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues

  Bonsch, B., Belt, V., Bartel, C., Duensing, N., Koziol, M., Lazarus, C. M., Bailey, A. M., Simpson, T. J. & Cox, R. J., 21 May 2016, In: Chemical Communications. 52, 41, p. 6777-6780 4 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Gene encoding 100%
  • Acyltransferases 36%
  • In Vitro Techniques 12%

  63 Citations (Scopus)

  337 Downloads (Pure)

• 2015

  Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

  Song, Z., Bakeer, W. I. M. M., Marshall, J. W., Yakasai, A. A., Mohd Khalid, R., Collemare, J., Skellam, E., Tharreau, D., Lebrun, M. H., Lazarus, C. M., Bailey, A. M., Simpson, T. J. & Cox, R. J., 1 Aug 2015, In: Chemical Science. 6, 8, p. 4837-4845 9 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Infectious Agent 100%
  • Heterologous Expression 100%
  • Magnaporthe Oryzae 100%
  • Oryza 100%
  • Virulence 100%

  74 Citations (Scopus)

  478 Downloads (Pure)

• Kinetic characterisation of the FAD dependent monooxygenase TropB and investigation of its biotransformation potential

  Cox, R., Abood, A., Al Fahad, A., Scott, A., Hosny, A.E.-D., Hashem, A., Fattah, A., Race, P. & Simpson, T., 2015, In: RSC Advances. 5, 62, p. 49987-49995

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  • Aromatic Structure 100%
  • Monooxygenase 100%
  • Enzyme 100%
  • Biotransformation 100%
  • Unspecific Monooxygenase 100%

  24 Citations (Scopus)

• Novel nonadride, heptadride and maleic acid metabolites from the byssochlamic acid producer Byssochlamys fulva IMI 40021 – an insight into the biosynthesis of maleidrides

  Szwalbe, A. J., Williams, K., O'Flynn, D. E., Bailey, A. M., Mulholland, N. P., Vincent, J. L., Willis, C. L., Cox, R. J. & Simpson, T. J., 14 Dec 2015, In: Chemical Communications. 51, 96, p. 17088-17091 4 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Metabolite 100%
  • Maleic Acid 100%
  • Maleic Anhydride 100%
  • Byssochlamys Fulva 100%
  • Decarboxylation 33%

  31 Citations (Scopus)

  459 Downloads (Pure)

• 2014

  Biosynthesis of Mupirocin by Pseudomonas fluorescens NCIMB 10586 Involves Parallel Pathways

  Gao, S.-S., Hothersall, J., Wu, J., Murphy, A. C., Song, Z., Stephens, E. R., Thomas, C. M., Crump, M. P., Cox, R. J., Simpson, T. J. & Willis, C. L., 9 Apr 2014, In: Journal of the American Chemical Society. 136, 14, p. 5501-5507 7 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Anabolism 100%
  • Mupirocin 100%
  • Pseudomonas fluorescens 100%
  • Pseudomonic Acid 100%
  • Metabolite 66%

  38 Citations (Scopus)

  649 Downloads (Pure)

• Diverse and potentially manipulative signalling with ascarosides in the model nematode C. elegans

  Diaz, S. A., Brunet, V., Lloyd-Jones, G. C., Spinner, W., Wharam, B. & Viney, M., 11 Mar 2014, In: BMC Evolutionary Biology. 14, 8 p., 46.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  • Environmental Information 100%
  • Population Density 100%
  • Information System 100%
  • Dauer Larva 100%
  • Caenorhabditis Elegans 100%

  30 Citations (Scopus)

• Oxidative dearomatisation: the key step of sorbicillinoid biosynthesis

  al Fahad, A., Abood, A., Fisch, K. M., Osipow, A., Davison, J., Avramovic, M., Butts, C. P., Piel, J., Simpson, T. J. & Cox, R. J., 2014, In: Chemical Science. 5, 2, p. 523-527 5 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  • Penicillium chrysogenum 100%
  • Secondary Metabolite 100%
  • Escherichia coli 100%
  • Gene Cluster 100%

  79 Citations (Scopus)

• The Biosynthesis and Catabolism of the Maleic Anhydride Moiety of Stipitatonic Acid

  Cox, R. J., Al Fahad, A., Abood, A. & Simpson, T. J., 2014, In: Angewandte Chemie - International Edition. 53, 29, p. 7519-7523

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  29 Citations (Scopus)

• 2013

  A conserved motif flags acyl carrier proteins for β-branching in polyketide synthesis

  Haines, A. S., Dong, X., Song, Z., Farmer, R., Williams, C., Hothersall, J., Ploskon, E., Wattana-amorn, P., Stephens, E. R., Yamada, E., Gurney, R., Takebayashi, Y., Masschelein, J., Cox, R. J., Lavigne, R., Willis, C. L., Simpson, T. J., Crosby, J., Winn, P. J. & Thomas, C. M. & 1 others, Crump, M. P., Nov 2013, In: Nature Chemical Biology. 9, 11, p. 685-692 8 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  Open Access

  File

  • Polyketide 100%
  • Acyl Carrier Protein 100%
  • Synthase 25%
  • Hydroxymethylglutaryl-CoA Synthase 25%
  • Sequence Motif 16%

  56 Citations (Scopus)

  724 Downloads (Pure)

• Chemical mechanisms involved during the biosynthesis of tropolones

  Cox, R. J. & Al-Fahad, A., Aug 2013, In: Current Opinion in Chemical Biology. 17, 4, p. 532-536 5 p.

  Research output: Contribution to journal › Review article (Academic Journal) › peer-review

  • Tropolone 100%
  • Polyketide 50%
  • Ring Expansion 50%
  • Aromatic Structure 50%
  • Shikimate 50%

  34 Citations (Scopus)

• One pathway, many compounds: heterologous expression of a fungal biosynthetic pathway reveals its intrinsic potential for diversity

  Wasil, Z., Abdul Kadir Pahirulzaman, K., Butts, C. P., Simpson, T. J., Lazarus, C. M. & Cox, R. J., 2013, In: Chemical Science. 4, 10, p. 3845-3856 12 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  • Chemistry 100%
  • Polyketide 100%
  • Aspyridone A 100%
  • Aspyridone B 100%
  • Chemical Role 100%

  82 Citations (Scopus)

• 2012

  Genetic, molecular, and biochemical basis of fungal tropolone biosynthesis

  Davison, J., Al Fahad, A., Cai, M., Song, Z., Yehia, S. Y., Lazarus, C. M., Bailey, A. M., Simpson, T. J. & Cox, R. J., Jan 2012, In: Proceedings of the National Academy of Sciences of the United States of America. 109, 20, p. 7642-7647 6 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  • Anabolism 100%
  • Molecular Genetics 100%
  • Tropolone 100%
  • Hydroxylation 100%
  • Gene Cluster 66%

  154 Citations (Scopus)

• 2010

  Catalytic role of the C-terminal domains of a fungal non-reducing polyketide synthase

  Fisch, K., Skellam, E., Ivison, D., Cox, R., Bailey, A., Lazarus, C. & Simpson, T., 2010, In: Chemical Communications. 46, p. 5331 - 5333

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  30 Citations (Scopus)

• Mutation of key residues in the C-methyltransferase domain of a fungal highly reducing polyketide synthase

  Skellam, E., Hurley, D., Davison, J., Lazarus, C., Simpson, T. & Cox, R. J., 2010, In: Molecular bioSystems. 6(4), p. 680 - 682 3 p.

  Research output: Contribution to journal › Article (Academic Journal) › peer-review

  12 Citations (Scopus)