TY - JOUR
T1 - β-catenin perturbations control differentiation programs in mouse embryonic stem cells
AU - Pedone, Elisa
AU - Failli, Mario
AU - Gambardella, Gennaro
AU - De Cegli, Rossella
AU - La Regina, Antonella
AU - di Bernardo, Diego
AU - Marucci, Lucia
N1 - Funding Information:
We thank Dr Andre Hermann and Dr Lorena Sueiro Ballesteros (Flow Cytometry Facility, University of Bristol), Dr Mark Jepson and Alan Leard (Wolfson Imaging Facility, University of Bristol) and the Next Generation Sequencing Core (TIGEM, Naples) for their support. This work was funded by Medical Research Council (grant MR/N021444/1) to L.M. by the Engineering and Physical Sciences Research Council (grants EP/R041695/1 and EP/S01876X/1 to L.M.), EC funding H2020 (FET OPEN 766840-COSY-BIO) to L.M. BrisSynBio, a BBSRC/EPSRC Synthetic Biology Research Centre (BB/L01386X/1) to L.M. STAR-University of Naples Federico II grant to G.G. and Fondazione Telethon grant to D.d.B. E.P. designed and performed experiments; M.F. and G.G. performed the WGNCA analysis; M.F. performed the GO; R.D.C. performed the Differential Expression analysis; A.L.R. supported the experimental work; E.P. and L.M. analyzed data; E.P. M.F. R.D.C. and L.M. wrote the paper; D.d.B. supervised the bioinformatics analysis; L.M. supervised the entire project. The authors declare that they have no competing interests.
Funding Information:
We thank Dr Andre Hermann and Dr Lorena Sueiro Ballesteros (Flow Cytometry Facility, University of Bristol), Dr Mark Jepson and Alan Leard (Wolfson Imaging Facility, University of Bristol) and the Next Generation Sequencing Core (TIGEM, Naples) for their support. This work was funded by Medical Research Council (grant MR/N021444/1 ) to L.M., by the Engineering and Physical Sciences Research Council (grants EP/R041695/1 and EP/S01876X/1 to L.M.), EC funding H2020 (FET OPEN 766840-COSY-BIO) to L.M., BrisSynBio, a BBSRC / EPSRC Synthetic Biology Research Centre ( BB/L01386X/1 ) to L.M., STAR- University of Naples Federico II grant to G.G., and Fondazione Telethon grant to D.d.B.
Publisher Copyright:
© 2022 The Authors
PY - 2022/2/18
Y1 - 2022/2/18
N2 - The Wnt/β-catenin pathway is involved in development, cancer, and embryonic stem cell (ESC) maintenance; its dual role in stem cell self-renewal and differentiation is still controversial. Here, by applying an in vitro system enabling inducible gene expression control, we report that moderate induction of transcriptionally active exogenous β-catenin in β-catenin null mouse ESCs promotes epiblast-like cell (EpiLC) derivation in vitro. Instead, in wild-type cells, moderate chemical pre-activation of the Wnt/β-catenin pathway promotes EpiLC in vitro derivation. Finally, we suggest that moderate β-catenin levels in β-catenin null mouse ESCs favor early stem cell commitment toward mesoderm if the exogenous protein is induced only in the “ground state” of pluripotency condition, or endoderm if the induction is maintained during the differentiation. Overall, our results confirm previous findings about the role of β-catenin in pluripotency and differentiation, while indicating a role for its doses in promoting specific differentiation programs.
AB - The Wnt/β-catenin pathway is involved in development, cancer, and embryonic stem cell (ESC) maintenance; its dual role in stem cell self-renewal and differentiation is still controversial. Here, by applying an in vitro system enabling inducible gene expression control, we report that moderate induction of transcriptionally active exogenous β-catenin in β-catenin null mouse ESCs promotes epiblast-like cell (EpiLC) derivation in vitro. Instead, in wild-type cells, moderate chemical pre-activation of the Wnt/β-catenin pathway promotes EpiLC in vitro derivation. Finally, we suggest that moderate β-catenin levels in β-catenin null mouse ESCs favor early stem cell commitment toward mesoderm if the exogenous protein is induced only in the “ground state” of pluripotency condition, or endoderm if the induction is maintained during the differentiation. Overall, our results confirm previous findings about the role of β-catenin in pluripotency and differentiation, while indicating a role for its doses in promoting specific differentiation programs.
U2 - 10.1016/j.isci.2022.103756
DO - 10.1016/j.isci.2022.103756
M3 - Article (Academic Journal)
C2 - 35128356
SN - 2589-0042
VL - 25
JO - iScience
JF - iScience
IS - 2
M1 - 103756
ER -