3D Reconstruction of the Glycocalyx Structure in Mammalian Capillaries using Electron Tomography

KP Arkill, CR Neal, J Mantell, C Michel, K Qvortrup, J Rostgaard, DO Bates, C Knupp, JM Squire

Research output: Contribution to journalArticle (Academic Journal)peer-review

31 Citations (Scopus)

Abstract

Visualising the molecular strands making up the glycocalyx in the lumen of small blood vessels has proved to be difficult using conventional transmission electron microscopy techniques. Images obtained from tissue stained in a variety of ways have revealed a regularity in the organisation of the proteoglycan components of the glycocalyx layer (fundamental spacing about 20 nm), but require a large sample number. Attempts to visualise the glycocalyx face-on (i.e. in a direction perpendicular to the endothelial cell layer in the lumen and directly applicable for permeability modelling) has had limited success (e.g. freeze fracture). A new approach is therefore needed. Here we demonstrate the effectiveness of using the relatively novel electron microscopy technique of 3D electron tomography ontwo differently stained preparations to reveal details of the architecture of the glycocalyx just above the endothelial cell layer. One preparation uses the novel staining technique using Lanthanum Dysprosium Glycosamino Glycan adhesion (the LaDy GAGa method).
Translated title of the contribution3D Reconstruction of the Glycocalyx Structure in Mammalian Capillaries using Electron Tomography
Original languageEnglish
Pages (from-to)343 - 351
Number of pages8
JournalMicrocirculation
Volume19
DOIs
Publication statusPublished - May 2012

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