3D tumour models and time-lapse analysis by multidimensional microscopy

Nobue Itasaki, Dimitri Scholz

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

The 3D culture is advantageous in reflecting the in vivo condition compared to the 2D culture; however, imaging 3D-cultured cells may be a challenge due to technical restrictions. Recent development of confocal spinning disc microscope system as well as sophisticated software has enabled us to monitor dynamism of cell movement in multiple dimensions. Here we describe the method for time-lapse imaging of 3D-cultured cancer cells co-cultured with non-cancerous cells and discuss current limitations and future perspectives.
Original languageEnglish
Title of host publicationCancer Chemoprevention
Subtitle of host publicationMethods and Protocols
EditorsSabrina Strano
PublisherSpringer
Pages181-188
Number of pages8
ISBN (Electronic)9781493931910
ISBN (Print)9781493931903
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer New York
Volume1379
ISSN (Print)1064-3745

Keywords

  • Three-dimensional culture
  • Time-lapse imaging
  • Multidimensional microscopy
  • Live cell imaging
  • Deconvolution

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