3D tumour models and time-lapse analysis by multidimensional microscopy

Dimitri Scholz, Nobue Itasaki

Research output: Chapter in Book/Report/Conference proceedingChapter in a book


The 3D culture is advantageous in reflecting the in vivo condition compared to the 2D culture; however, imaging 3D-cultured cells may be a challenge due to technical restrictions. Recent development of confocal spinning disc microscope system as well as sophisticated software has enabled us to monitor dynamism of cell movement in multiple dimensions. Here we describe the method for time-lapse imaging of 3D-cultured cancer cells co-cultured with non-cancerous cells and discuss current limitations and future perspectives.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationCancer Chemoprevention
EditorsSabrina Strano
Number of pages8
ISBN (Electronic)9781493931910
ISBN (Print)9781493931903
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer New York
ISSN (Print)1064-3745


  • Three-dimensional culture
  • Time-lapse imaging
  • Multidimensional microscopy
  • Live cell imaging
  • Deconvolution


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