Abstract
BACKGROUND: Optical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution methods that allow capturing the full three-dimensional nature of biological systems and also can visualize multiple protein species in the same sample.
METHODOLOGY/PRINCIPAL FINDINGS: We show that the use of a combination of conventional near-infrared dyes, such as Alexa 647, Alexa 680 and Alexa 750, all excited with a 671 nm diode laser, enables 3D multi-colour super-resolution imaging of complex biological samples. Optically thick samples, including human tissue sections, cardiac rat myocytes and densely grown neuronal cultures were imaged with lateral resolutions of ∼15 nm (std. dev.) while reducing marker cross-talk to
| Original language | English |
|---|---|
| Article number | e20645 |
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | PLoS ONE |
| Volume | 6 |
| DOIs | |
| Publication status | Published - 2011 |
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Baddeley, D., Crossman, D., Rossberger, S., Cheyne, J., Montgomery, J., Isuru, D., Jayasinghe, ID., Cremer, C., Cannell, MB., & Soeller, C. (2011). 4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues. PLoS ONE, 6, 1-10. [e20645]. https://doi.org/10.1371/journal.pone.0020645