4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues

D Baddeley, D Crossman, S Rossberger, J Cheyne, J Montgomery, D Isuru, ID Jayasinghe, C Cremer, MB Cannell, C Soeller

Research output: Contribution to journalArticle (Academic Journal)

94 Citations (Scopus)

Abstract

BACKGROUND: Optical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution methods that allow capturing the full three-dimensional nature of biological systems and also can visualize multiple protein species in the same sample. METHODOLOGY/PRINCIPAL FINDINGS: We show that the use of a combination of conventional near-infrared dyes, such as Alexa 647, Alexa 680 and Alexa 750, all excited with a 671 nm diode laser, enables 3D multi-colour super-resolution imaging of complex biological samples. Optically thick samples, including human tissue sections, cardiac rat myocytes and densely grown neuronal cultures were imaged with lateral resolutions of ∼15 nm (std. dev.) while reducing marker cross-talk to
Original languageEnglish
Article numbere20645
Pages (from-to)1-10
Number of pages10
JournalPLoS ONE
Volume6
DOIs
Publication statusPublished - 2011

Fingerprint Dive into the research topics of '4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues'. Together they form a unique fingerprint.

  • Cite this

    Baddeley, D., Crossman, D., Rossberger, S., Cheyne, J., Montgomery, J., Isuru, D., Jayasinghe, ID., Cremer, C., Cannell, MB., & Soeller, C. (2011). 4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues. PLoS ONE, 6, 1-10. [e20645]. https://doi.org/10.1371/journal.pone.0020645