Projects per year
Abstract
Combined quantum mechanics/molecular mechanics (QM/MM) simulations of the reaction catalysed by phosphite dehydrogenase (PTDH) identify Met53 as important for catalysis. This catalytic role is verified by experiments (including replacement by norleucine and selenomethionine), which show that mutation of this residue significantly affects k(cat), without changing K-M for phosphite. QM/MM and ab initio QM calculations show that the catalytic effect is electrostatic in nature. The side chain of Met53 specifically stabilizes the transition state for the hydride transfer step of the reaction catalysed by PTDH, forming a 'face-on' interaction with His292. To our knowledge, a defined catalytic role for methionine in an enzyme (as opposed to a steric or binding effect, or interaction with a metal ion) has not previously been identified. Analyses of the Protein Data Bank and Cambridge Structural Database indicate that this type of interaction may be relatively widespread, with implications for enzyme-catalysed reaction mechanisms and protein structure.
Original language | English |
---|---|
Pages (from-to) | 2191-2199 |
Number of pages | 9 |
Journal | Chemical Science |
Volume | 5 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- BIOMOLECULAR SYSTEMS
- ENZYME CATALYSIS
- QM/MM METHODS
- AMINO-ACID
- PROTEINS
- DYNAMICS
- RESIDUES
- REGENERATION
- SULFUR
- ATOM
Fingerprint
Dive into the research topics of 'A catalytic role for methionine revealed by a combination of computation and experiments on phosphite dehydrogenase'. Together they form a unique fingerprint.Projects
- 4 Finished
-
CCP-BioSim: Biomolecular simulation at the life sciences interface
Mulholland, A. J. (Principal Investigator)
1/10/11 → 1/10/15
Project: Research
-
Electron Delocalization in Peptides and Proteins
Woolfson, D. N. (Principal Investigator)
1/10/11 → 1/10/14
Project: Research
-
COMBINED EXPERIMENTAL AND COMPUTATIONAL INVESTIGATIONS OF A NUCLEOPHILLIC DISPLACEMENT REACTION WITH A HYDRIDE LEAVING GROUP
Mulholland, A. J. (Principal Investigator)
1/04/09 → 1/04/12
Project: Research
Profiles
-
Professor Adrian J Mulholland
- Infection and Immunity
- School of Chemistry - Professor
Person: Academic , Member