A comparative study on the clinical, parasitological and molecular diagnosis of bovine trypanosomosis in Uganda

JW Magona*, JSP Mayende, W Olaho-Mukani, PG Coleman, NN Jonsson, SC Welburn, MC Eisler

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

14 Citations (Scopus)

Abstract

The clinical, parasitological and molecular diagnosis of bovine trypanosomosis were compared using samples from 250 zebu cattle exposed to natural trypanosome challenge in Uganda. Clinical examination, molecular and parasitological diagnoses detected 184 (73.6%), 96 (38.4%) and 36 (14.4%) as diseased, respectively. The sensitivity and specificity of clinical examination were 87.5% and 35%, and 78% and 27 % based on molecular and parasitological diagnoses, as gold standards, respectively. Of the 33, 3, 13 and 12 parasitological-positive cattle that had Trypanosoma brucei, Trypanosoma congolense, Trypanosoma vivax or mixed infections, 78%, 33 %, 84% and 100% respectively manifested clinical signs. Of the 24, 89, 12, 3, 6 and 27 cattle detected by molecular diagnosis to have mixed infections, T. brucei, T. vivax, T. congolense forest-, Savannah- and Tsavo-type, 100%, 83%, 91%, 100%, 67% and 81 % had clinical signs, respectively. In conclusion, treatment of cattle based on clinical examination may clear up to 87.5% or 78% of the cases that would be positive by either molecular or parasitological diagnosis, respectively. Under field conditions, in the absence of simple and portable diagnostic tools or access to laboratory facilities, veterinarians could rely on clinical diagnosis to screen and treat cases of bovine trypanosomosis presented by farmers before confirmatory diagnosis in diagnostic centres for few unclear cases is sought.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalOnderstepoort Journal of Veterinary Research
Volume70
Issue number3
Publication statusPublished - Sep 2003

Keywords

  • cattle
  • diagnosis
  • trypanosomosis
  • POLYMERASE-CHAIN-REACTION
  • INFECTED CATTLE
  • TSETSE-FLIES
  • DNA PROBES
  • CONGOLENSE
  • VIVAX
  • BLOOD
  • BRUCEI
  • KENYA
  • ELISA

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