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A Composite Hydrogel Scaffold Permits Self‐Organization and Matrix Deposition by Cocultured Human Glomerular Cells

Research output: Contribution to journalArticle

Original languageEnglish
Article number1900698
Number of pages11
JournalAdvanced Healthcare Materials
Volume8
Issue number17
Early online date30 Jul 2019
DOIs
DateAccepted/In press - 15 Jul 2019
DateE-pub ahead of print - 30 Jul 2019
DatePublished (current) - 5 Sep 2019

Abstract

Three-dimensional scaffolds provide cells with a spatial environment that more closely resembles that of in vivo tissue, when compared to 2D culture on a plastic substrate. However, many scaffolding materials commonly used in tissue engineering tend to exhibit anisotropic morphologies that exhibit a narrow range of fibre diameters and pore-sizes, which do not recapitulate extracellular matrices. In this study, a fibrin hydrogel is formed within the interstitial spaces of an electrospun poly(glycolic) acid (PGA) monolith to generate a composite, bimodal scaffold for the co-culture of kidney glomerular cell lines. This new scaffold exhibits multiple fibre morphologies, containing both PGA microfibres (14.5 ± 2 µm) and fibrin gel nanofibres (0.14 ± 0.09 µm), which increase the compressive Young’s modulus beyond that of either of the constituents. The composite structure provides an enhanced 3D environment that increases proliferation and adhesion of immortalised human podocytes and glomerular endothelial cells. Moreover, the micro/nanoscale fibrous morphology promotes motility and reorganisation of the glomerular cells into glomerulus-like structures, resulting in the deposition of organised collagen IV; the primary component of the glomerular basement membrane (GBM).

    Research areas

  • glomerulus, kidneys, renal, scaffolds, tissue engineering

Documents

Documents

  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Wiley at https://doi.org/10.1002/adhm.201900698 . Please refer to any applicable terms of use of the publisher.

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    Embargo ends: 30/07/20

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  • Supplementary information PDF

    Accepted author manuscript, 1 MB, PDF document

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