A De Novo Virus-Like Topology for Synthetic Virions

James Noble, Emiliana De Santis, Jascinda Ravi, Baptiste Lamarre, Valeria Castelletto, Judith Mantell, Santanu Ray, Maxim Ryadnov

Research output: Contribution to journalArticle (Academic Journal)

32 Citations (Scopus)

Abstract

A de novo topology of virus-like assembly is reported. The design is a trifaceted coiled-coil peptide helix, which self-assembles into ultrasmall, monodisperse, anionic virus-like shells that encapsulate and transfer both RNA and DNA into human cells. Unlike existing artificial systems, these shells share the same physical characteristics of viruses being anionic, nonaggregating, abundant, hollow, and uniform in size, while effectively mediating gene silencing and transgene expression. These are the smallest virus-like structures reported to date, both synthetic and native, with the ability to adapt and transfer small and large nucleic acids. The design thus offers a promising solution for engineering bespoke artificial viruses with desired functions.
Original languageEnglish
Pages (from-to)12202–12210
Number of pages9
JournalJournal of the American Chemical Society
Volume37
Issue number138
Early online date1 Sep 2016
DOIs
Publication statusPublished - 26 Sep 2016

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    Noble, J., De Santis, E., Ravi, J., Lamarre, B., Castelletto, V., Mantell, J., Ray, S., & Ryadnov, M. (2016). A De Novo Virus-Like Topology for Synthetic Virions. Journal of the American Chemical Society, 37(138), 12202–12210. https://doi.org/10.1021/jacs.6b05751