A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease

J Puleston; M Cooper; S Murch; K Bid; S Makh; P Ashwood; A H Bingham; H Green; P Moss; A Dhillon; R Morris; S Strobel; R Gelinas; R E Pounder; A Platt

doi:10.1111/j.1365-2036.2004.02262.x

A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease

J Puleston
, M Cooper
, S Murch
, K Bid
, S Makh
, P Ashwood
, A H Bingham
, H Green
, P Moss
, A Dhillon
, R Morris
, S Strobel
, R Gelinas
, R E Pounder
, A Platt

Bristol Medical School (PHS)

Research output: Contribution to journal › Article (Academic Journal) › peer-review

110 Citations (Scopus)

Abstract

BACKGROUND: Inflammatory bowel disease (IBD) is characterised by intense mucosal recruitment of activated leukocytes. Chemokines determine inflammatory leukocyte recruitment and retention.

AIM: To compare expression of the entire chemokine family within colonic mucosa from IBD patients and uninflamed controls.

METHODS: A microarray of cDNAs, representing every member of this superfamily and their cognate receptors, was hybridised with probes derived from colonoscopic biopsies.

RESULTS: A distinct subset of chemokines, consisting of CXCLs 1-3 and 8 and CCL20, was upregulated in active colonic IBD, compared with uninflamed areas or tissue from controls. Increased expression of their cognate receptors, CXCR1, CXCR2 and CCR6, was confirmed by quantitative PCR and immunohistochemistry. An identical chemokine response was induced in Caco-2 cells by stimulation with interleukin (IL)-1beta, but not tumour necrosis factor-alpha (TNF-alpha). By contrast, IL-1beta and TNF-alpha were synergistic in an HT29 cell line and primary keratinocytes.

CONCLUSIONS: IL-1beta and TNF-alpha appear to be the pivotal mediators of a previously unidentified coordinated epithelial chemokine response that dominates the mucosal chemokine environment in inflamed IBD tissue.

Original language	English
Pages (from-to)	109-20
Number of pages	12
Journal	Alimentary Pharmacology and Therapeutics
Volume	21
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2036.2004.02262.x
Publication status	Published - 15 Jan 2005

Keywords

Caco-2 Cells
Chemokines
Flow Cytometry
Humans
Inflammatory Bowel Diseases
Interleukin-1
Intestinal Mucosa
Tumor Necrosis Factor-alpha
Up-Regulation

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Puleston, J., Cooper, M., Murch, S., Bid, K., Makh, S., Ashwood, P., Bingham, A. H., Green, H., Moss, P., Dhillon, A., Morris, R., Strobel, S., Gelinas, R., Pounder, R. E., & Platt, A. (2005). A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease. Alimentary Pharmacology and Therapeutics, 21(2), 109-20. https://doi.org/10.1111/j.1365-2036.2004.02262.x

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title = "A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease",

abstract = "BACKGROUND: Inflammatory bowel disease (IBD) is characterised by intense mucosal recruitment of activated leukocytes. Chemokines determine inflammatory leukocyte recruitment and retention.AIM: To compare expression of the entire chemokine family within colonic mucosa from IBD patients and uninflamed controls.METHODS: A microarray of cDNAs, representing every member of this superfamily and their cognate receptors, was hybridised with probes derived from colonoscopic biopsies.RESULTS: A distinct subset of chemokines, consisting of CXCLs 1-3 and 8 and CCL20, was upregulated in active colonic IBD, compared with uninflamed areas or tissue from controls. Increased expression of their cognate receptors, CXCR1, CXCR2 and CCR6, was confirmed by quantitative PCR and immunohistochemistry. An identical chemokine response was induced in Caco-2 cells by stimulation with interleukin (IL)-1beta, but not tumour necrosis factor-alpha (TNF-alpha). By contrast, IL-1beta and TNF-alpha were synergistic in an HT29 cell line and primary keratinocytes.CONCLUSIONS: IL-1beta and TNF-alpha appear to be the pivotal mediators of a previously unidentified coordinated epithelial chemokine response that dominates the mucosal chemokine environment in inflamed IBD tissue.",

keywords = "Caco-2 Cells, Chemokines, Flow Cytometry, Humans, Inflammatory Bowel Diseases, Interleukin-1, Intestinal Mucosa, Tumor Necrosis Factor-alpha, Up-Regulation",

author = "J Puleston and M Cooper and S Murch and K Bid and S Makh and P Ashwood and Bingham, \{A H\} and H Green and P Moss and A Dhillon and R Morris and S Strobel and R Gelinas and Pounder, \{R E\} and A Platt",

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doi = "10.1111/j.1365-2036.2004.02262.x",

language = "English",

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Puleston, J, Cooper, M, Murch, S, Bid, K, Makh, S, Ashwood, P, Bingham, AH, Green, H, Moss, P, Dhillon, A, Morris, R, Strobel, S, Gelinas, R, Pounder, RE & Platt, A 2005, 'A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease', Alimentary Pharmacology and Therapeutics, vol. 21, no. 2, pp. 109-20. https://doi.org/10.1111/j.1365-2036.2004.02262.x

TY - JOUR

T1 - A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease

AU - Puleston, J

AU - Cooper, M

AU - Murch, S

AU - Bid, K

AU - Makh, S

AU - Ashwood, P

AU - Bingham, A H

AU - Green, H

AU - Moss, P

AU - Dhillon, A

AU - Morris, R

AU - Strobel, S

AU - Gelinas, R

AU - Pounder, R E

AU - Platt, A

PY - 2005/1/15

Y1 - 2005/1/15

N2 - BACKGROUND: Inflammatory bowel disease (IBD) is characterised by intense mucosal recruitment of activated leukocytes. Chemokines determine inflammatory leukocyte recruitment and retention.AIM: To compare expression of the entire chemokine family within colonic mucosa from IBD patients and uninflamed controls.METHODS: A microarray of cDNAs, representing every member of this superfamily and their cognate receptors, was hybridised with probes derived from colonoscopic biopsies.RESULTS: A distinct subset of chemokines, consisting of CXCLs 1-3 and 8 and CCL20, was upregulated in active colonic IBD, compared with uninflamed areas or tissue from controls. Increased expression of their cognate receptors, CXCR1, CXCR2 and CCR6, was confirmed by quantitative PCR and immunohistochemistry. An identical chemokine response was induced in Caco-2 cells by stimulation with interleukin (IL)-1beta, but not tumour necrosis factor-alpha (TNF-alpha). By contrast, IL-1beta and TNF-alpha were synergistic in an HT29 cell line and primary keratinocytes.CONCLUSIONS: IL-1beta and TNF-alpha appear to be the pivotal mediators of a previously unidentified coordinated epithelial chemokine response that dominates the mucosal chemokine environment in inflamed IBD tissue.

AB - BACKGROUND: Inflammatory bowel disease (IBD) is characterised by intense mucosal recruitment of activated leukocytes. Chemokines determine inflammatory leukocyte recruitment and retention.AIM: To compare expression of the entire chemokine family within colonic mucosa from IBD patients and uninflamed controls.METHODS: A microarray of cDNAs, representing every member of this superfamily and their cognate receptors, was hybridised with probes derived from colonoscopic biopsies.RESULTS: A distinct subset of chemokines, consisting of CXCLs 1-3 and 8 and CCL20, was upregulated in active colonic IBD, compared with uninflamed areas or tissue from controls. Increased expression of their cognate receptors, CXCR1, CXCR2 and CCR6, was confirmed by quantitative PCR and immunohistochemistry. An identical chemokine response was induced in Caco-2 cells by stimulation with interleukin (IL)-1beta, but not tumour necrosis factor-alpha (TNF-alpha). By contrast, IL-1beta and TNF-alpha were synergistic in an HT29 cell line and primary keratinocytes.CONCLUSIONS: IL-1beta and TNF-alpha appear to be the pivotal mediators of a previously unidentified coordinated epithelial chemokine response that dominates the mucosal chemokine environment in inflamed IBD tissue.

KW - Caco-2 Cells

KW - Chemokines

KW - Flow Cytometry

KW - Humans

KW - Inflammatory Bowel Diseases

KW - Interleukin-1

KW - Intestinal Mucosa

KW - Tumor Necrosis Factor-alpha

KW - Up-Regulation

U2 - 10.1111/j.1365-2036.2004.02262.x

DO - 10.1111/j.1365-2036.2004.02262.x

M3 - Article (Academic Journal)

C2 - 15679760

SN - 0269-2813

VL - 21

SP - 109

EP - 120

JO - Alimentary Pharmacology and Therapeutics

JF - Alimentary Pharmacology and Therapeutics

IS - 2

ER -

A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease

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