A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana

Reuben W Nowell; Ben Elsworth; Vicencio Oostra; Bas J Zwaan; Christopher W Wheat; Marjo Saastamoinen; Ilik J Saccheri; Arjen E Van't Hof; Bethany R Wasik; Heidi Connahs; Muhammad L Aslam; Sujai Kumar; Richard J Challis; Antónia Monteiro; Paul M Brakefield; Mark Blaxter

doi:10.1093/gigascience/gix035

A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana

Reuben W Nowell, Ben Elsworth, Vicencio Oostra, Bas J Zwaan, Christopher W Wheat, Marjo Saastamoinen, Ilik J Saccheri, Arjen E Van't Hof, Bethany R Wasik, Heidi Connahs, Muhammad L Aslam, Sujai Kumar, Richard J Challis, Antónia Monteiro, Paul M Brakefield, Mark Blaxter

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Abstract

Background: The mycalesine butterfly Bicyclus anynana , the 'Squinting bush brown', is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species.

Findings: Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology. 128 Gb raw Illumina data were filtered to 124 Gb and assembled to a final size of 475 Mb (∼260X assembly coverage). Contigs were scaffolded using mate-pair, transcriptome and PacBio data into 10,800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements, and encodes a total of 22,642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes.

Conclusions: We report a high-quality draft genome sequence for Bicyclus anynana . The genome assembly and annotated gene models are available at LepBase ( http://ensembl.lepbase.org/index.html ).

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	GigaScience
Volume	6
Issue number	7
Early online date	9 May 2017
DOIs	https://doi.org/10.1093/gigascience/gix035
Publication status	Published - Jul 2017

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Nowell, R. W., Elsworth, B., Oostra, V., Zwaan, B. J., Wheat, C. W., Saastamoinen, M., Saccheri, I. J., Van't Hof, A. E., Wasik, B. R., Connahs, H., Aslam, M. L., Kumar, S., Challis, R. J., Monteiro, A., Brakefield, P. M., & Blaxter, M. (2017). A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana. GigaScience, 6(7), 1-7. https://doi.org/10.1093/gigascience/gix035

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title = "A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana",

abstract = "Background: The mycalesine butterfly Bicyclus anynana , the 'Squinting bush brown', is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species.Findings: Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology. 128 Gb raw Illumina data were filtered to 124 Gb and assembled to a final size of 475 Mb (∼260X assembly coverage). Contigs were scaffolded using mate-pair, transcriptome and PacBio data into 10,800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements, and encodes a total of 22,642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes.Conclusions: We report a high-quality draft genome sequence for Bicyclus anynana . The genome assembly and annotated gene models are available at LepBase ( http://ensembl.lepbase.org/index.html ).",

author = "Nowell, {Reuben W} and Ben Elsworth and Vicencio Oostra and Zwaan, {Bas J} and Wheat, {Christopher W} and Marjo Saastamoinen and Saccheri, {Ilik J} and {Van't Hof}, {Arjen E} and Wasik, {Bethany R} and Heidi Connahs and Aslam, {Muhammad L} and Sujai Kumar and Challis, {Richard J} and Ant{\'o}nia Monteiro and Brakefield, {Paul M} and Mark Blaxter",

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doi = "10.1093/gigascience/gix035",

language = "English",

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AU - Nowell, Reuben W

AU - Elsworth, Ben

AU - Oostra, Vicencio

AU - Zwaan, Bas J

AU - Wheat, Christopher W

AU - Saastamoinen, Marjo

AU - Saccheri, Ilik J

AU - Van't Hof, Arjen E

AU - Wasik, Bethany R

AU - Connahs, Heidi

AU - Aslam, Muhammad L

AU - Kumar, Sujai

AU - Challis, Richard J

AU - Monteiro, Antónia

AU - Brakefield, Paul M

AU - Blaxter, Mark

PY - 2017/7

Y1 - 2017/7

N2 - Background: The mycalesine butterfly Bicyclus anynana , the 'Squinting bush brown', is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species.Findings: Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology. 128 Gb raw Illumina data were filtered to 124 Gb and assembled to a final size of 475 Mb (∼260X assembly coverage). Contigs were scaffolded using mate-pair, transcriptome and PacBio data into 10,800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements, and encodes a total of 22,642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes.Conclusions: We report a high-quality draft genome sequence for Bicyclus anynana . The genome assembly and annotated gene models are available at LepBase ( http://ensembl.lepbase.org/index.html ).

AB - Background: The mycalesine butterfly Bicyclus anynana , the 'Squinting bush brown', is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species.Findings: Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology. 128 Gb raw Illumina data were filtered to 124 Gb and assembled to a final size of 475 Mb (∼260X assembly coverage). Contigs were scaffolded using mate-pair, transcriptome and PacBio data into 10,800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements, and encodes a total of 22,642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes.Conclusions: We report a high-quality draft genome sequence for Bicyclus anynana . The genome assembly and annotated gene models are available at LepBase ( http://ensembl.lepbase.org/index.html ).

U2 - 10.1093/gigascience/gix035

DO - 10.1093/gigascience/gix035

M3 - Article (Academic Journal)

C2 - 28486658

SN - 2047-217X

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SP - 1

EP - 7

JO - GigaScience

JF - GigaScience

IS - 7

ER -

A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana

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