A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in Wistar rats

Luciana Simão do Carmo, Marcelo Macedo Rogero, Edgar Julian Paredes-Gamero, Amanda Nogueira-Pedro, Jose Guilherme Xavier, Mayara Cortez, Maria Carolina Borges, Tatiane Mieko Fujii, Primavera Borelli, Ricardo Ambrósio Fock

Research output: Contribution to journalArticle (Academic Journal)peer-review

21 Citations (Scopus)

Abstract

It is well established that the excessive consumption of a high-fat diet (HFD) results in overweight, obesity and an increase in leptin concentrations, which triggers a chronic inflammatory condition that is associated with a high white blood cell count. Two-month-old male Wistar rats were fed a control (CON) diet or an HFD for 12 weeks. After this period, hemogram, myelogram and biochemical parameters were evaluated along with the cell cycle and the percentage of CD34(+) cells in the bone marrow as well as cell proliferation and differentiation assays and the production of stem cell factor, interleukin 3 (IL-3), granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). The HFD animals exhibited leukocytosis and neutrophilia with increased C-reactive protein, leptin, cholesterol and triglyceride concentrations. In the HFD group, the bone marrow revealed myeloid hyperplasia, especially of the granulocytic compartment with a higher percentage of CD34(+) cells and a higher percentage of cells in the G2/S/M cell cycle phases. In addition, the HFD bone marrow cells had a higher capacity to proliferate and differentiate into granulocytic cells in an in vitro system and a higher capacity to produce IL-3 and G-CSF. These data led us to infer that the HFD induces leukocytosis and neutrophilia suggesting alterations in hematopoiesis system modulation.

Original languageEnglish
Pages (from-to)375-84
Number of pages10
JournalExperimental Biology and Medicine
Volume238
Issue number4
DOIs
Publication statusPublished - Apr 2013

Keywords

  • Animals
  • Bone Marrow Cells
  • C-Reactive Protein
  • Cells, Cultured
  • Cholesterol
  • Diet, High-Fat
  • Dietary Fats
  • Granulocyte Colony-Stimulating Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Hyperplasia
  • In Vitro Techniques
  • Interleukin-3
  • Leptin
  • Leukocytosis
  • Male
  • Models, Animal
  • Neutrophils
  • Rats
  • Rats, Wistar
  • Stem Cell Factor
  • Triglycerides

Fingerprint

Dive into the research topics of 'A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in Wistar rats'. Together they form a unique fingerprint.

Cite this