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A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants

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A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants. / Coudert, Yoan; Novák, Ondřej; Harrison, C. Jill.

In: Current Biology, Vol. 29, No. 16, 19.08.2019, p. 2743-2750.e5.

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Coudert, Yoan ; Novák, Ondřej ; Harrison, C. Jill. / A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants. In: Current Biology. 2019 ; Vol. 29, No. 16. pp. 2743-2750.e5.

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@article{b2ee739b6ad7466d84e1dad5a353bca7,
title = "A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants",
abstract = "The diverse forms of today's dominant vascular plant flora are generated by the sustained proliferative activity of sporophyte meristems at plants’ shoot and root tips, a trait known as indeterminacy [1]. Bryophyte sister lineages to the vascular plants lack such indeterminate meristems and have an overall sporophyte form comprising a single small axis that ceases growth in the formation of a reproductive sporangium [1]. Genetic mechanisms regulating indeterminacy are well characterized in flowering plants, involving a feedback loop between class I KNOX genes and cytokinin [2, 3], and class I KNOX expression is a conserved feature of vascular plant meristems [4]. The transition from determinate growth to indeterminacy during evolution was a pre-requisite to vascular plant diversification, but mechanisms enabling the innovation of indeterminacy are unknown [5]. Here, we show that class I KNOX gene activity is necessary and sufficient for axis extension from an intercalary region of determinate moss shoots. As in Arabidopsis, class I KNOX activity can promote cytokinin biosynthesis by an ISOPENTENYL TRANSFERASE gene, PpIPT3. PpIPT3 promotes axis extension, and PpIPT3 and exogenously applied cytokinin can partially compensate for loss of class I KNOX function. By outgroup comparison, the results suggest that a pre-existing KNOX-cytokinin regulatory module was recruited into vascular plant shoot meristems during evolution to promote indeterminacy, thereby enabling the radiation of vascular plant shoot forms.",
keywords = "plant evolution, evo-devo, vascular plant origins, ISOPENTENYL TRANSFERASE, indeterminacy, KNOX-cytokinin",
author = "Yoan Coudert and Ondřej Nov{\'a}k and Harrison, {C. Jill}",
year = "2019",
month = "8",
day = "19",
doi = "10.1016/j.cub.2019.06.083",
language = "English",
volume = "29",
pages = "2743--2750.e5",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "16",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants

AU - Coudert, Yoan

AU - Novák, Ondřej

AU - Harrison, C. Jill

PY - 2019/8/19

Y1 - 2019/8/19

N2 - The diverse forms of today's dominant vascular plant flora are generated by the sustained proliferative activity of sporophyte meristems at plants’ shoot and root tips, a trait known as indeterminacy [1]. Bryophyte sister lineages to the vascular plants lack such indeterminate meristems and have an overall sporophyte form comprising a single small axis that ceases growth in the formation of a reproductive sporangium [1]. Genetic mechanisms regulating indeterminacy are well characterized in flowering plants, involving a feedback loop between class I KNOX genes and cytokinin [2, 3], and class I KNOX expression is a conserved feature of vascular plant meristems [4]. The transition from determinate growth to indeterminacy during evolution was a pre-requisite to vascular plant diversification, but mechanisms enabling the innovation of indeterminacy are unknown [5]. Here, we show that class I KNOX gene activity is necessary and sufficient for axis extension from an intercalary region of determinate moss shoots. As in Arabidopsis, class I KNOX activity can promote cytokinin biosynthesis by an ISOPENTENYL TRANSFERASE gene, PpIPT3. PpIPT3 promotes axis extension, and PpIPT3 and exogenously applied cytokinin can partially compensate for loss of class I KNOX function. By outgroup comparison, the results suggest that a pre-existing KNOX-cytokinin regulatory module was recruited into vascular plant shoot meristems during evolution to promote indeterminacy, thereby enabling the radiation of vascular plant shoot forms.

AB - The diverse forms of today's dominant vascular plant flora are generated by the sustained proliferative activity of sporophyte meristems at plants’ shoot and root tips, a trait known as indeterminacy [1]. Bryophyte sister lineages to the vascular plants lack such indeterminate meristems and have an overall sporophyte form comprising a single small axis that ceases growth in the formation of a reproductive sporangium [1]. Genetic mechanisms regulating indeterminacy are well characterized in flowering plants, involving a feedback loop between class I KNOX genes and cytokinin [2, 3], and class I KNOX expression is a conserved feature of vascular plant meristems [4]. The transition from determinate growth to indeterminacy during evolution was a pre-requisite to vascular plant diversification, but mechanisms enabling the innovation of indeterminacy are unknown [5]. Here, we show that class I KNOX gene activity is necessary and sufficient for axis extension from an intercalary region of determinate moss shoots. As in Arabidopsis, class I KNOX activity can promote cytokinin biosynthesis by an ISOPENTENYL TRANSFERASE gene, PpIPT3. PpIPT3 promotes axis extension, and PpIPT3 and exogenously applied cytokinin can partially compensate for loss of class I KNOX function. By outgroup comparison, the results suggest that a pre-existing KNOX-cytokinin regulatory module was recruited into vascular plant shoot meristems during evolution to promote indeterminacy, thereby enabling the radiation of vascular plant shoot forms.

KW - plant evolution

KW - evo-devo

KW - vascular plant origins

KW - ISOPENTENYL TRANSFERASE

KW - indeterminacy

KW - KNOX-cytokinin

UR - http://www.scopus.com/inward/record.url?scp=85070615480&partnerID=8YFLogxK

U2 - 10.1016/j.cub.2019.06.083

DO - 10.1016/j.cub.2019.06.083

M3 - Article

VL - 29

SP - 2743-2750.e5

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 16

ER -