Activities per year
Abstract
Aims: Present in ~70% of new-onset diabetes cases, autoantibodies to zinc transporter 8 (ZnT8A) are one of the four major markers of islet autoimmunity used to identify high-risk individuals. We sought to compare the performance of a novel luciferase-based ZnT8 immunoassay (Nluc-ZnT8) with the “gold standard” radioimmunoassay.
Method: The Nluc-ZnT8 was evaluated using samples previously tested by ZnT8A radioimmunoassay from 589 new-onset diabetes [sampled within 3 months of diagnosis; 329 males; median diagnosis age 11 years (range 1-55)] and 300 relatives who progressed to diabetes [median follow-up 12.4 years (range 0.2-31); 127 males; median age at sample 28 years (range 1.4-64.1]. Positivity thresholds were set at the 97.5th percentile of 523 healthy schoolchildren (range 9.0-14years). Units were derived from a logarithmic standard curve.
Results: Levels of autoantibodies detected by Nluc-ZnT8 and radioimmunoassay were highly correlated [Spearman’s R= 0.90; p<0.0001]. In ZnT8A radioimmunoassay positives, 379/402 (94.3%) were Nluc-ZnT8 positive. Of 187 radioimmunoassay negatives, an additional 31 (17%) new-onset diabetes were identified by Nluc-ZnT8. The majority of discrepant samples (80%) had low level ZnT8A (<3 units) and therefore, may be explained by detection limits and assay format. In relatives positive by both assays, the 20-year diabetes risk was 58%. Of 60 ZnT8A radioimmunoassay positive relatives and, 240 radioimmunoassay negative relatives, 54 (90%) and 30 (13%) were positive respectively, by Nluc-ZnT8.
Conclusion: This Nluc-ZnT8 LIPs assay provides a cheaper, higher throughput, lower serum volume alternative to radioimmunoassay that can discriminate diabetes risk and therefore, should facilitate large-scale screening for type 1 diabetes risk.
Original language | English |
---|---|
Publication status | Published - 19 Apr 2021 |
Event | Diabetes UK Professional Conference 2021 - Online Duration: 16 Apr 2021 → 30 Apr 2021 https://www.diabetes.org.uk/diabetes-uk-professional-conference |
Conference
Conference | Diabetes UK Professional Conference 2021 |
---|---|
Period | 16/04/21 → 30/04/21 |
Internet address |
Fingerprint
Dive into the research topics of 'A low-volume rapid luciferase immunoprecipitation system (LIPS) assay utilising a novel antigen to detect autoantibodies to zinc transporter 8 (ZnT8A) in type 1 diabetes provides an alternative to conventional radioimmunoassay'. Together they form a unique fingerprint.Student theses
-
The natural history of the autoimmune response to zinc transporter 8 (ZnT8) in type 1 diabetes
Williams, C. L. (Author), Long, A. E. (Supervisor), Williams, A. J. K. (Supervisor) & Gillespie, K. M. (Supervisor), 25 Jan 2022Student thesis: Doctoral Thesis › Doctor of Philosophy (PhD)
File
Prizes
-
Diabetes UK Basic Science Digital Poster Award 2021
Williams, C. L. (Recipient), 30 Apr 2021
Prize: Prizes, Medals, Awards and Grants
File
Activities
- 1 Participation in conference
-
Diabetes UK Professional Conference 2021
Williams, C. L. (Participant)
16 Apr 2021 → 30 Apr 2021Activity: Participating in or organising an event types › Participation in conference