A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

Mahmoud Khazim; Elisa Pedone; Lorena Postiglione; Diego di Bernardo; Lucia Marucci

doi:10.1007/978-1-0716-1032-9_10

A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

Mahmoud Khazim, Elisa Pedone, Lorena Postiglione, Diego di Bernardo, Lucia Marucci

Research output: Contribution to journal › Article (Academic Journal) › peer-review

4 Citations (Scopus)

Abstract

Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.

Original language	English
Pages (from-to)	205-219
Number of pages	15
Journal	Methods in Molecular Biology
Volume	2229
Early online date	7 Jan 2021
DOIs	https://doi.org/10.1007/978-1-0716-1032-9_10
Publication status	Published - 2021

Bibliographical note

Funding Information:
This work was funded by Medical Research Council grant MR/N021444/1 to L.M., by the Engineering and Physical Sciences Research Council grants EP/R041695/1 and EP/S01876X/1 to L.M., and by BrisSynBio, a BBSRC/EPSRC Synthetic Biology Research Centre (BB/L01386X/1) to L.M.

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Research Groups and Themes

Engineering Mathematics Research Group

Keywords

Algorithms
Animals
Gene Expression
Genetic Engineering
Humans
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques/instrumentation

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10.1007/978-1-0716-1032-9_10

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Unravelling the role of beta-catenin in ground state pluripotency
Marucci, L. (Principal Investigator)
1/09/16 → 29/02/20
Project: Research

Cite this

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title = "A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells",

abstract = "Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.",

keywords = "Algorithms, Animals, Gene Expression, Genetic Engineering, Humans, Lab-On-A-Chip Devices, Microfluidic Analytical Techniques/instrumentation",

author = "Mahmoud Khazim and Elisa Pedone and Lorena Postiglione and {di Bernardo}, Diego and Lucia Marucci",

note = "Funding Information: This work was funded by Medical Research Council grant MR/N021444/1 to L.M., by the Engineering and Physical Sciences Research Council grants EP/R041695/1 and EP/S01876X/1 to L.M., and by BrisSynBio, a BBSRC/EPSRC Synthetic Biology Research Centre (BB/L01386X/1) to L.M. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

doi = "10.1007/978-1-0716-1032-9_10",

language = "English",

volume = "2229",

pages = "205--219",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

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}

TY - JOUR

T1 - A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

AU - Khazim, Mahmoud

AU - Pedone, Elisa

AU - Postiglione, Lorena

AU - di Bernardo, Diego

AU - Marucci, Lucia

N1 - Funding Information: This work was funded by Medical Research Council grant MR/N021444/1 to L.M., by the Engineering and Physical Sciences Research Council grants EP/R041695/1 and EP/S01876X/1 to L.M., and by BrisSynBio, a BBSRC/EPSRC Synthetic Biology Research Centre (BB/L01386X/1) to L.M. Publisher Copyright: © 2021, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2021

Y1 - 2021

N2 - Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.

AB - Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.

KW - Algorithms

KW - Animals

KW - Gene Expression

KW - Genetic Engineering

KW - Humans

KW - Lab-On-A-Chip Devices

KW - Microfluidic Analytical Techniques/instrumentation

U2 - 10.1007/978-1-0716-1032-9_10

DO - 10.1007/978-1-0716-1032-9_10

M3 - Article (Academic Journal)

C2 - 33405224

SN - 1064-3745

VL - 2229

SP - 205

EP - 219

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

Abstract

Bibliographical note

Research Groups and Themes

Keywords

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Projects

Unravelling the role of beta-catenin in ground state pluripotency

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