A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

Mahmoud Khazim, Elisa Pedone, Lorena Postiglione, Diego di Bernardo, Lucia Marucci

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.

Original languageEnglish
Pages (from-to)205-219
Number of pages15
JournalMethods in Molecular Biology
Volume2229
DOIs
Publication statusPublished - 2021

Bibliographical note

Funding Information:
This work was funded by Medical Research Council grant MR/N021444/1 to L.M., by the Engineering and Physical Sciences Research Council grants EP/R041695/1 and EP/S01876X/1 to L.M., and by BrisSynBio, a BBSRC/EPSRC Synthetic Biology Research Centre (BB/L01386X/1) to L.M.

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Algorithms
  • Animals
  • Gene Expression
  • Genetic Engineering
  • Humans
  • Lab-On-A-Chip Devices
  • Microfluidic Analytical Techniques/instrumentation

Fingerprint

Dive into the research topics of 'A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells'. Together they form a unique fingerprint.

Cite this