A Modular Vaccine Platform Combining Self‐Assembled Peptide Cages and Immunogenic Peptides

Caroline Morris, Sarah Glennie, Hon Lam, Holly Baum, Dhinushi Kandage, Neil Williams, David Morgan, Dek Woolfson, Andrew Davidson

Research output: Contribution to journalArticle (Academic Journal)peer-review

8 Citations (Scopus)
151 Downloads (Pure)

Abstract

Subunit vaccines use delivery platforms to present minimal antigenic compo- nents for immunization. The benefits of such systems include multivalency, self-adjuvanting properties, and more specific immune responses. Previously, the design, synthesis, and characterization of self-assembling peptide cages (SAGEs) have been reported. In these, de novo peptides are combined to make hubs that assemble into nanoparticles when mixed in aqueous solu- tion. Here it is shown that SAGEs are nontoxic particles with potential as accessible synthetic peptide scaffolds for the delivery of immunogenic com- ponents. To this end, SAGEs functionalized with the model antigenic peptides tetanus toxoid632-651 and ovalbumin323-339 drive antigen-specific responses both in vitro and in vivo, eliciting both CD4+ T cell and B cell responses. Additionally, SAGEs functionalized with the antigenic peptide hemag- glutinin518-526 from the influenza virus are also able to drive a CD8+ T cell response in vivo. This work demonstrates the potential of SAGEs to act as
a modular scaffold for antigen delivery, capable of inducing and boosting specific and tailored immune responses.
Original languageEnglish
Article number1807357
Number of pages12
JournalAdvanced Functional Materials
Volume29
Issue number8
Early online date11 Jan 2019
DOIs
Publication statusPublished - 21 Feb 2019

Structured keywords

  • Bristol BioDesign Institute
  • BrisSynBio

Keywords

  • coiled coils
  • peptide design
  • self-assembly
  • subunit vaccines
  • Synthetic biology

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