A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis

Christopher T Evans; Loren M Picco; Oliver D Payton; Mike Allen; John  Hardy; Sara  Baldock

doi:10.3390/microorganisms9040680

A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis

Christopher T Evans, Loren M Picco, Oliver D Payton, Mike Allen^*, John Hardy, Sara Baldock

^*Corresponding author for this work

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Abstract

Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min.

Original language	English
Article number	680
Journal	Microorganisms
Volume	9
Issue number	4
DOIs	https://doi.org/10.3390/microorganisms9040680
Publication status	Published - 25 Mar 2021

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title = "A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis",

abstract = "Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min.",

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AU - Evans, Christopher T

AU - Picco, Loren M

AU - Payton, Oliver D

AU - Allen, Mike

AU - Hardy, John

AU - Baldock, Sara

PY - 2021/3/25

Y1 - 2021/3/25

N2 - Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min.

AB - Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min.

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DO - 10.3390/microorganisms9040680

M3 - Article (Academic Journal)

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JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 4

M1 - 680

ER -

A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis

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