A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland

K E Gordon, B Binas, R S Chapman, K M Kurian, R W Clarkson, A J Clark, E B Lane, C J Watson

Research output: Contribution to journalArticle (Academic Journal)peer-review

37 Citations (Scopus)

Abstract

This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine beta-lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag).
Original languageEnglish
Pages (from-to)222-35
Number of pages14
JournalBreast Cancer Research
Volume2
Issue number3
DOIs
Publication statusPublished - 2000

Keywords

  • Animals
  • Apoptosis
  • Recombinant Proteins
  • Breast
  • Cell Culture Techniques
  • Cell Differentiation
  • Mice
  • Mice, Transgenic
  • Transcriptional Activation
  • Lactoglobulins
  • Pregnancy
  • Promoter Regions, Genetic
  • Caseins
  • Antigens, Viral, Tumor
  • Genetic Vectors
  • Simian virus 40
  • Milk Proteins
  • Epithelium
  • Cell Line, Transformed
  • Gene Dosage
  • Signal Transduction
  • Biological Markers
  • Female

Fingerprint Dive into the research topics of 'A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland'. Together they form a unique fingerprint.

Cite this