A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland

K E Gordon; B Binas; R S Chapman; K M Kurian; R W Clarkson; A J Clark; E B Lane; C J Watson

doi:10.1186/bcr57

A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland

K E Gordon, B Binas, R S Chapman, K M Kurian, R W Clarkson, A J Clark, E B Lane, C J Watson

Research output: Contribution to journal › Article (Academic Journal) › peer-review

40 Citations (Scopus)

Abstract

This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine beta-lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag).

Original language	English
Pages (from-to)	222-35
Number of pages	14
Journal	Breast Cancer Research
Volume	2
Issue number	3
DOIs	https://doi.org/10.1186/bcr57
Publication status	Published - 2000

Keywords

Animals
Apoptosis
Recombinant Proteins
Breast
Cell Culture Techniques
Cell Differentiation
Mice
Mice, Transgenic
Transcriptional Activation
Lactoglobulins
Pregnancy
Promoter Regions, Genetic
Caseins
Antigens, Viral, Tumor
Genetic Vectors
Simian virus 40
Milk Proteins
Epithelium
Cell Line, Transformed
Gene Dosage
Signal Transduction
Biological Markers
Female

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title = "A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland",

abstract = "This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine beta-lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag).",

keywords = "Animals, Apoptosis, Recombinant Proteins, Breast, Cell Culture Techniques, Cell Differentiation, Mice, Mice, Transgenic, Transcriptional Activation, Lactoglobulins, Pregnancy, Promoter Regions, Genetic, Caseins, Antigens, Viral, Tumor, Genetic Vectors, Simian virus 40, Milk Proteins, Epithelium, Cell Line, Transformed, Gene Dosage, Signal Transduction, Biological Markers, Female",

author = "Gordon, \{K E\} and B Binas and Chapman, \{R S\} and Kurian, \{K M\} and Clarkson, \{R W\} and Clark, \{A J\} and Lane, \{E B\} and Watson, \{C J\}",

year = "2000",

doi = "10.1186/bcr57",

language = "English",

volume = "2",

pages = "222--35",

journal = "Breast Cancer Research",

issn = "1465-5411",

publisher = "BioMed Central Ltd",

number = "3",

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TY - JOUR

T1 - A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland

AU - Gordon, K E

AU - Binas, B

AU - Chapman, R S

AU - Kurian, K M

AU - Clarkson, R W

AU - Clark, A J

AU - Lane, E B

AU - Watson, C J

PY - 2000

Y1 - 2000

N2 - This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine beta-lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag).

AB - This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine beta-lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag).

KW - Animals

KW - Apoptosis

KW - Recombinant Proteins

KW - Breast

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Mice

KW - Mice, Transgenic

KW - Transcriptional Activation

KW - Lactoglobulins

KW - Pregnancy

KW - Promoter Regions, Genetic

KW - Caseins

KW - Antigens, Viral, Tumor

KW - Genetic Vectors

KW - Simian virus 40

KW - Milk Proteins

KW - Epithelium

KW - Cell Line, Transformed

KW - Gene Dosage

KW - Signal Transduction

KW - Biological Markers

KW - Female

U2 - 10.1186/bcr57

DO - 10.1186/bcr57

M3 - Article (Academic Journal)

C2 - 11056687

SN - 1465-5411

VL - 2

SP - 222

EP - 235

JO - Breast Cancer Research

JF - Breast Cancer Research

IS - 3

ER -

A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland

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