Projects per year
Abstract
The polymorphic nature of G-quadruplex (G4) DNA structures points to a range of potential applications in nanodevices and an opportunity to control G4 in biological settings. Light is an attractive means for the regulation of oligonucleotide structure as it can be delivered with high spatiotemporal precision. However, surprisingly little attention has been devoted towards the development of ligands for G4 that allow photoregulation of G4 folding. We report a novel G4-binding chemotype derived from stiff-stilbene. Surprisingly however, whilst the ligand induces high stabilization in the potassium form of human telomeric DNA, it causes the unfolding of the same G4 sequence in sodium buffer. This effect can be reversed on demand by irradiation with 400 nm light through deactivation of the ligand by photo-oxidation. By fuelling the system with the photolabile ligand, the conformation of G4 DNA was switched five times.
Original language | English |
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Pages (from-to) | 4334-4338 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 58 |
Issue number | 13 |
Early online date | 20 Feb 2019 |
DOIs | |
Publication status | Published - 22 Mar 2019 |
Structured keywords
- BCS and TECS CDTs
Keywords
- circular dichroism
- DNA
- G-quadruplexes
- oligonucleotides
- photoregulation
Fingerprint
Dive into the research topics of 'A Photoresponsive Stiff-Stilbene Ligand Fuels the Reversible Unfolding of G-Quadruplex DNA'. Together they form a unique fingerprint.Projects
- 3 Finished
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Elucidation of energy and charge transportation mechanisms in biomolecules and nanomaterials
1/12/17 → 31/03/21
Project: Research
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Exploring Delocalised Energy Transport in Bacterial Reaction Centres
1/02/17 → 31/07/18
Project: Research
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Predicting drug-target binding kinetics through multiscale simulations
1/05/15 → 30/04/19
Project: Research
Equipment
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HPC (High Performance Computing) Facility
Sadaf R Alam (Manager), Steven A Chapman (Manager), Polly E Eccleston (Other), Simon H Atack (Other) & D A G Williams (Manager)
Facility/equipment: Facility
Profiles
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Professor M C Galan
- School of Chemistry - Professor of Organic and Biological Chemistry
- Synthesis
- Catalysis
- Biological and Archaeological Chemistry
Person: Academic , Member