A Protocol for a Novel Human Ex Vivo Model of Aneurysm

Rosaria Bianco*, Karina Di Gregoli, Massimo Caputo, Sarah J George, Jason L Johnson*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

19 Downloads (Pure)

Abstract

Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.

Original languageEnglish
Pages (from-to)100108
Number of pages15
JournalSTAR Protocols
Volume1
Issue number3
Early online date9 Sep 2020
DOIs
Publication statusPublished - 18 Dec 2020

Keywords

  • aneurysm
  • model
  • 3Rs

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