A Protocol for a Novel Human Ex Vivo Model of Aneurysm

Rosaria Bianco; Karina Di Gregoli; Massimo Caputo; Sarah J George; Jason L Johnson

doi:10.1016/j.xpro.2020.100108

A Protocol for a Novel Human Ex Vivo Model of Aneurysm

Rosaria Bianco^*, Karina Di Gregoli, Massimo Caputo, Sarah J George, Jason L Johnson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.

Original language	English
Pages (from-to)	100108
Number of pages	15
Journal	STAR Protocols
Volume	1
Issue number	3
Early online date	9 Sept 2020
DOIs	https://doi.org/10.1016/j.xpro.2020.100108
Publication status	Published - 18 Dec 2020

Structured keywords

Bristol Heart Institute

Keywords

aneurysm
model
3Rs

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2020.100108

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Development and characterisation of a human ex vivo model of aneurysm
Johnson, J. L. & George, S. J.
15/02/16 → 14/02/19
Project: Research

Cite this

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title = "A Protocol for a Novel Human Ex Vivo Model of Aneurysm",

abstract = "Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.",

keywords = "aneurysm, model, 3Rs",

author = "Rosaria Bianco and {Di Gregoli}, Karina and Massimo Caputo and George, {Sarah J} and Johnson, {Jason L}",

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T1 - A Protocol for a Novel Human Ex Vivo Model of Aneurysm

AU - Bianco, Rosaria

AU - Di Gregoli, Karina

AU - Caputo, Massimo

AU - George, Sarah J

AU - Johnson, Jason L

PY - 2020/12/18

Y1 - 2020/12/18

N2 - Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.

AB - Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.

KW - aneurysm

KW - model

KW - 3Rs

U2 - 10.1016/j.xpro.2020.100108

DO - 10.1016/j.xpro.2020.100108

M3 - Article (Academic Journal)

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SP - 100108

JO - STAR Protocols

JF - STAR Protocols

IS - 3

ER -

A Protocol for a Novel Human Ex Vivo Model of Aneurysm

Abstract

Structured keywords

Keywords

UN SDGs

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Development and characterisation of a human ex vivo model of aneurysm

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