A scalable workflow to test "shock and kill" therapeutic approaches against the HIV-1 latent reservoir in blood cells ex vivo

Francesco Procopio; Andrea Savarino; Iart Luca Shytaj

doi:10.1016/j.xpro.2023.102253

A scalable workflow to test "shock and kill" therapeutic approaches against the HIV-1 latent reservoir in blood cells ex vivo

Francesco Procopio, Andrea Savarino, Iart Luca Shytaj^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Abstract

Integrated HIV-1 DNA persists in cells of people living with HIV during antiretroviral treatment, but its quantification is hindered by its rarity. Here, we present an optimized protocol to evaluate “shock and kill” therapeutic strategies, including both the latency reactivation (“shock”) and elimination of infected cells (“kill”) stages. We describe steps for the sequential use of nested PCR-based assays and viability sorting to allow for scalable and rapid screening of candidate therapeutics in patient-derived blood cells.

Original language	English
Article number	102253
Number of pages	27
Journal	STAR Protocols
Volume	4
Issue number	2
Early online date	24 Apr 2023
DOIs	https://doi.org/10.1016/j.xpro.2023.102253
Publication status	Published - 16 Jun 2023

Bibliographical note

Funding Information:
A.S. acknowledges intramural support from the Italian Institute of Health . The authors thank Dr. Lara Gallucci for helpful suggestions.

Publisher Copyright:
© 2023 The Authors

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abstract = "Integrated HIV-1 DNA persists in cells of people living with HIV during antiretroviral treatment, but its quantification is hindered by its rarity. Here, we present an optimized protocol to evaluate “shock and kill” therapeutic strategies, including both the latency reactivation (“shock”) and elimination of infected cells (“kill”) stages. We describe steps for the sequential use of nested PCR-based assays and viability sorting to allow for scalable and rapid screening of candidate therapeutics in patient-derived blood cells.",

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A scalable workflow to test "shock and kill" therapeutic approaches against the HIV-1 latent reservoir in blood cells ex vivo

Abstract

Bibliographical note

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