A serine protease triad forms the catalytic centre of a triacylglycerol lipase

Leo Brady, Andrzej M. Brzozowski, Zygmunt S. Derewenda*, Eleanor Dodson, Guy Dodson, Shirley Tolley, Johan P. Turkenburg, Lars Christiansen, Birgitte Huge-Jensen, Leif Norskov, Lars Thim, Ulrich Menge

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

1025 Citations (Scopus)

Abstract

True Upases attack triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.

Original languageEnglish
Pages (from-to)767-770
Number of pages4
JournalNature
Volume343
Issue number6260
DOIs
Publication statusPublished - 1 Jan 1990

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