TY - JOUR
T1 - A serine protease triad forms the catalytic centre of a triacylglycerol lipase
AU - Brady, Leo
AU - Brzozowski, Andrzej M.
AU - Derewenda, Zygmunt S.
AU - Dodson, Eleanor
AU - Dodson, Guy
AU - Tolley, Shirley
AU - Turkenburg, Johan P.
AU - Christiansen, Lars
AU - Huge-Jensen, Birgitte
AU - Norskov, Leif
AU - Thim, Lars
AU - Menge, Ulrich
PY - 1990/1/1
Y1 - 1990/1/1
N2 - True Upases attack triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
AB - True Upases attack triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
UR - http://www.scopus.com/inward/record.url?scp=0025057072&partnerID=8YFLogxK
U2 - 10.1038/343767a0
DO - 10.1038/343767a0
M3 - Article (Academic Journal)
C2 - 2304552
AN - SCOPUS:0025057072
SN - 0028-0836
VL - 343
SP - 767
EP - 770
JO - Nature
JF - Nature
IS - 6260
ER -