A simple method for the determination of reduction potentials in heme proteins

Igor Efimov, Gary Parkin, Elizabeth S. Millett, Jennifer Glenday, Cheuk K. Chan, Holly Weedon, Harpreet Randhawa, Jaswir Basran, Emma L. Raven*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

29 Citations (Scopus)

Abstract

We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em = -115 mV ± 2 mV, pH 7.0) and human CLOCK (Em = -111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

Original languageEnglish
Pages (from-to)701-704
Number of pages4
JournalFEBS Letters
Volume588
Issue number5
DOIs
Publication statusPublished - 3 Mar 2014

Keywords

  • Heme
  • Heme protein
  • Redox
  • Reduction potential

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