Abstract
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em = -115 mV ± 2 mV, pH 7.0) and human CLOCK (Em = -111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
Original language | English |
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Pages (from-to) | 701-704 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 588 |
Issue number | 5 |
DOIs | |
Publication status | Published - 3 Mar 2014 |
Keywords
- Heme
- Heme protein
- Redox
- Reduction potential