A simple method for the determination of reduction potentials in heme proteins

Igor Efimov; Gary Parkin; Elizabeth S. Millett; Jennifer Glenday; Cheuk K. Chan; Holly Weedon; Harpreet Randhawa; Jaswir Basran; Emma L. Raven

doi:10.1016/j.febslet.2013.12.030

A simple method for the determination of reduction potentials in heme proteins

Igor Efimov, Gary Parkin, Elizabeth S. Millett, Jennifer Glenday, Cheuk K. Chan, Holly Weedon, Harpreet Randhawa, Jaswir Basran, Emma L. Raven^*

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article (Academic Journal) › peer-review

49 Citations (Scopus)

Abstract

We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E_m = -115 mV ± 2 mV, pH 7.0) and human CLOCK (E_m = -111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

Original language	English
Pages (from-to)	701-704
Number of pages	4
Journal	FEBS Letters
Volume	588
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2013.12.030
Publication status	Published - 3 Mar 2014

Research Groups and Themes

Inorganic & Materials

Keywords

Heme
Heme protein
Redox
Reduction potential

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title = "A simple method for the determination of reduction potentials in heme proteins",

abstract = "We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em = -115 mV ± 2 mV, pH 7.0) and human CLOCK (Em = -111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.",

keywords = "Heme, Heme protein, Redox, Reduction potential",

author = "Igor Efimov and Gary Parkin and Millett, \{Elizabeth S.\} and Jennifer Glenday and Chan, \{Cheuk K.\} and Holly Weedon and Harpreet Randhawa and Jaswir Basran and Raven, \{Emma L.\}",

year = "2014",

month = mar,

day = "3",

doi = "10.1016/j.febslet.2013.12.030",

language = "English",

volume = "588",

pages = "701--704",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

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TY - JOUR

T1 - A simple method for the determination of reduction potentials in heme proteins

AU - Efimov, Igor

AU - Parkin, Gary

AU - Millett, Elizabeth S.

AU - Glenday, Jennifer

AU - Chan, Cheuk K.

AU - Weedon, Holly

AU - Randhawa, Harpreet

AU - Basran, Jaswir

AU - Raven, Emma L.

PY - 2014/3/3

Y1 - 2014/3/3

N2 - We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em = -115 mV ± 2 mV, pH 7.0) and human CLOCK (Em = -111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

AB - We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em = -115 mV ± 2 mV, pH 7.0) and human CLOCK (Em = -111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

KW - Heme

KW - Heme protein

KW - Redox

KW - Reduction potential

UR - https://www.scopus.com/pages/publications/84896712248

U2 - 10.1016/j.febslet.2013.12.030

DO - 10.1016/j.febslet.2013.12.030

M3 - Article (Academic Journal)

C2 - 24440354

AN - SCOPUS:84896712248

SN - 0014-5793

VL - 588

SP - 701

EP - 704

JO - FEBS Letters

JF - FEBS Letters

IS - 5

ER -

A simple method for the determination of reduction potentials in heme proteins

Abstract

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Keywords

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