A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome

Valeryia Kuzmuk, Iwona Pranke, Ruth Rollason, Matthew Butler, Wen Y Ding, Matthew Beesley, Aoife M Waters, Richard J Coward, Richard Sessions, Jack Tuffin, Rebecca R Foster, Géraldine Mollet, Corinne Antignac, Aleksander Edelman, Gavin I Welsh, Moin A Saleem

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Podocin is a key membrane scaffolding protein of the kidney podocyte essential for intact glomerular filtration. Mutations in NPHS2, the podocin-encoding gene, represent the commonest form of inherited nephrotic syndrome (NS), with early, intractable kidney failure. The most frequent podocin gene mutation in European children is R138Q, causing retention of the misfolded protein in the endoplasmic reticulum. Here, we provide evidence that podocin R138Q (but not wild-type podocin) complexes with the intermediate filament protein keratin 8 (K8) thereby preventing its correct trafficking to the plasma membrane. We have also identified a small molecule (c407), a compound that corrects the Cystic Fibrosis Transmembrane Conductance Regulator protein defect, that interrupts this complex and rescues mutant protein mistrafficking. This results in both the correct localization of podocin at the plasma membrane and functional rescue in both human patient R138Q mutant podocyte cell lines, and in a mouse inducible knock-in model of the R138Q mutation. Importantly, complete rescue of proteinuria and histological changes was seen when c407 was administered both via osmotic minipumps or delivered orally prior to induction of disease or crucially via osmotic minipump two weeks after disease induction. Thus, our data constitute a therapeutic option for patients with NS bearing a podocin mutation, with implications for other misfolding protein disorders. Further studies are necessary to confirm our findings.

Original languageEnglish
JournalKidney International
Early online date22 Nov 2023
DOIs
Publication statusE-pub ahead of print - 22 Nov 2023

Bibliographical note

Copyright © 2023 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.

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