Projects per year
Abstract
BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.
METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6 mm diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma and urine. Three miRNAs (RNU6B, let7b and miR-21RNA) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma and urine. Secondarily, candidate miRNAs let7b, miR21, miR-29b, miR-124 and miR-155 in DBS were evaluated as potential biomarkers for HIE.
RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs levels in DBS and EDTA-blood at -80°C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.
CONCLUSIONS: Dried blood spots may be useful for studying the potential of miRNAs as biomarkers for brain injury.Pediatric Research (2015); doi:10.1038/pr.2015.276.
Original language | English |
---|---|
Pages (from-to) | 799-805 |
Number of pages | 7 |
Journal | Pediatric Research |
Volume | 79 |
Issue number | 5 |
Early online date | 27 Jan 2016 |
DOIs | |
Publication status | Published - May 2016 |
Fingerprint
Dive into the research topics of 'A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method'. Together they form a unique fingerprint.Projects
- 1 Finished
-
REGULATION OF GENE EXPRESSION BY MICRORNAS DURING NERVE REGENERATION
Wong, L.-F. (Principal Investigator)
1/02/08 → 1/04/12
Project: Research