A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method

Vennila Ponnusamy; Olga Kapellou; Ellen Yip; Jane Evanson; Liang-Fong Wong; Adina Michael-Titus; Ping K Yip; Divyen K Shah

doi:10.1038/pr.2015.276

A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method

Vennila Ponnusamy, Olga Kapellou, Ellen Yip, Jane Evanson, Liang-Fong Wong, Adina Michael-Titus, Ping K Yip, Divyen K Shah

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.

METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6 mm diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma and urine. Three miRNAs (RNU6B, let7b and miR-21RNA) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma and urine. Secondarily, candidate miRNAs let7b, miR21, miR-29b, miR-124 and miR-155 in DBS were evaluated as potential biomarkers for HIE.

RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs levels in DBS and EDTA-blood at -80°C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.

CONCLUSIONS: Dried blood spots may be useful for studying the potential of miRNAs as biomarkers for brain injury.Pediatric Research (2015); doi:10.1038/pr.2015.276.

Original language	English
Pages (from-to)	799-805
Number of pages	7
Journal	Pediatric Research
Volume	79
Issue number	5
Early online date	27 Jan 2016
DOIs	https://doi.org/10.1038/pr.2015.276
Publication status	Published - May 2016

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REGULATION OF GENE EXPRESSION BY MICRORNAS DURING NERVE REGENERATION
Wong, L.
1/02/08 → 1/04/12
Project: Research

Cite this

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title = "A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method",

abstract = "BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6 mm diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma and urine. Three miRNAs (RNU6B, let7b and miR-21RNA) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma and urine. Secondarily, candidate miRNAs let7b, miR21, miR-29b, miR-124 and miR-155 in DBS were evaluated as potential biomarkers for HIE.RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs levels in DBS and EDTA-blood at -80°C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.CONCLUSIONS: Dried blood spots may be useful for studying the potential of miRNAs as biomarkers for brain injury.Pediatric Research (2015); doi:10.1038/pr.2015.276.",

author = "Vennila Ponnusamy and Olga Kapellou and Ellen Yip and Jane Evanson and Liang-Fong Wong and Adina Michael-Titus and Yip, {Ping K} and Shah, {Divyen K}",

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A study of microRNAs from dried blood spots in newborns after perinatal asphyxia : a simple and feasible biosampling method. / Ponnusamy, Vennila; Kapellou, Olga; Yip, Ellen; Evanson, Jane; Wong, Liang-Fong; Michael-Titus, Adina; Yip, Ping K; Shah, Divyen K.

In: Pediatric Research, Vol. 79, No. 5, 05.2016, p. 799-805.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

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T2 - a simple and feasible biosampling method

AU - Ponnusamy, Vennila

AU - Kapellou, Olga

AU - Yip, Ellen

AU - Evanson, Jane

AU - Wong, Liang-Fong

AU - Michael-Titus, Adina

AU - Yip, Ping K

AU - Shah, Divyen K

PY - 2016/5

Y1 - 2016/5

N2 - BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6 mm diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma and urine. Three miRNAs (RNU6B, let7b and miR-21RNA) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma and urine. Secondarily, candidate miRNAs let7b, miR21, miR-29b, miR-124 and miR-155 in DBS were evaluated as potential biomarkers for HIE.RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs levels in DBS and EDTA-blood at -80°C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.CONCLUSIONS: Dried blood spots may be useful for studying the potential of miRNAs as biomarkers for brain injury.Pediatric Research (2015); doi:10.1038/pr.2015.276.

AB - BACKGROUND: The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.METHODS: We describe a technique to extract and compare expression of miRNAs from a single small 6 mm diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma and urine. Three miRNAs (RNU6B, let7b and miR-21RNA) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma and urine. Secondarily, candidate miRNAs let7b, miR21, miR-29b, miR-124 and miR-155 in DBS were evaluated as potential biomarkers for HIE.RESULTS: Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs levels in DBS and EDTA-blood at -80°C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.CONCLUSIONS: Dried blood spots may be useful for studying the potential of miRNAs as biomarkers for brain injury.Pediatric Research (2015); doi:10.1038/pr.2015.276.

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DO - 10.1038/pr.2015.276

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SP - 799

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JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 5

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A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method

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REGULATION OF GENE EXPRESSION BY MICRORNAS DURING NERVE REGENERATION

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