A Syndecan-4 hair trigger initiates wound healing through Caveolin- and RhoG-regulated integrin endocytosis

M.D Bass, R.C Williamson, R.D Nunan, J.D Humphries, A Byron, M.R Morgan, P Martin, M.J Humphries

Research output: Contribution to journalArticle (Academic Journal)

87 Citations (Scopus)

Abstract

Cell migration during wound healing requires adhesion receptor turnover to enable the formation and disassembly of cell-extracellular matrix contacts. Although recent advances have improved our understanding of integrin trafficking pathways, it is not known how extracellular ligand engagement controls receptor dynamics. Using atomic force microscopy, we have measured cell avidity for fibronectin and defined a mechanism for the outside-in regulation of αsub>5β1-integrin. Surprisingly, adhesive strength was attenuated by the syndecan-4-binding domain of fibronectin due to a rapid triggering of αsub>5β1-integrin endocytosis. Association of syndecan-4 with PKCα was found to trigger RhoG activation and subsequent dynamin- and caveolin-dependent integrin uptake. Like disruption of syndecan-4 or caveolin, gene disruption of RhoG in mice was found to retard closure of dermal wounds due to a migration defect of the fibroblasts and keratinocytes of RhoG null mice. Thus, this syndecan-4-regulated integrin endocytic pathway appears to play a key role in tissue repair.
Translated title of the contributionA Syndecan-4 hair trigger initiates wound healing through Caveolin- and RhoG-regulated integrin endocytosis
Original languageEnglish
Pages (from-to)681 - 693
Number of pages13
JournalDevelopmental Cell
Volume21
Issue number5
DOIs
Publication statusPublished - Oct 2011

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