A Yeast Synthetic Network for In Vivo Assessment of Reverse-Engineering and Modeling Approaches

I. Cantone, Lucia Marucci, F. Iorio, M. Bansal, V. Belcastro, M.A. Ricci, S. Santini, M Di Bernardo, D. di Bernardo, M.P. Cosma

Research output: Contribution to journalArticle (Academic Journal)peer-review

284 Citations (Scopus)

Abstract

Systems biology approaches are extensively used to model and reverse engineer gene regulatory networks from experimental data. Conversely, synthetic biology allows “de novo” construction of a regulatory network to seed new functions in the cell. At present, the usefulness and predictive ability of modeling and reverse engineering cannot be assessed and compared rigorously. We built in the yeast Saccharomyces cerevisiae a synthetic network, IRMA, for in vivo “benchmarking” of reverse-engineering and modeling approaches. The network is composed of five genes regulating each other through a variety of regulatory interactions; it is negligibly affected by endogenous genes, and it is responsive to small molecules. We measured time series and steady-state expression data after multiple perturbations. These data were used to assess state-of-the-art modeling and reverse-engineering techniques. A semiquantitative model was able to capture and predict the behavior of the network. Reverse engineering based on differential equations and Bayesian networks correctly inferred regulatory interactions from the experimental data.
Translated title of the contributionA Yeast Synthetic Network for In Vivo Assessment of Reverse-Engineering and Modeling Approaches
Original languageEnglish
Pages (from-to)172–181
JournalCell
Volume137
DOIs
Publication statusPublished - Apr 2009

Structured keywords

  • Bristol BioDesign Institute

Keywords

  • synthetic biology

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