Aberrations in DNA methylation are detectable during remission of acute lymphoblastic leukemia and predict patient outcome

Sanne D van Otterdijk; Jean Norden; Anne M Dickinson; Mark S Pearce; Caroline L Relton; John C Mathers; Gordon Strathdee

doi:10.2217/epi.14.78

Aberrations in DNA methylation are detectable during remission of acute lymphoblastic leukemia and predict patient outcome

Sanne D van Otterdijk, Jean Norden, Anne M Dickinson, Mark S Pearce, Caroline L Relton, John C Mathers, Gordon Strathdee

Research output: Contribution to journal › Article (Academic Journal) › peer-review

4 Citations (Scopus)

Abstract

AIM: Aberrant DNA methylation patterns are a hallmark of cancer, although the extent to which they underlie cancer development is unknown. In this study, we aimed to determine whether acute lymphoblastic leukemia (ALL) patients in clinical remission retained abnormal DNA methylation patters and whether these were associated with patient outcome.

MATERIALS & METHODS: We investigated CpG island methylation of genes known to exhibit hypermethylation in leukemia using quantitative pyrosequencing analysis.

RESULTS: Although methylation levels were reduced in remission samples, they remained significantly higher than those seen in healthy controls. This retained methylation was not related to low levels of residual leukemia cells still present at remission. Methylation levels were also stable (or increased) during continuous remission and significantly correlated with long-term survival in adult ALL patients.

CONCLUSION: This study determined that abnormalities in DNA methylation are retained during ALL remission and may represent a novel prognostic marker for adult ALL patients.

Original language	English
Pages (from-to)	35-45
Number of pages	11
Journal	Epigenomics
Volume	7
Issue number	1
DOIs	https://doi.org/10.2217/epi.14.78
Publication status	Published - Feb 2015

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1 Finished

MRC UoB UNITE Unit - Programme 2
Relton, C. L. & Relton, C. L.
1/06/13 → 31/03/18
Project: Research

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title = "Aberrations in DNA methylation are detectable during remission of acute lymphoblastic leukemia and predict patient outcome",

abstract = "AIM: Aberrant DNA methylation patterns are a hallmark of cancer, although the extent to which they underlie cancer development is unknown. In this study, we aimed to determine whether acute lymphoblastic leukemia (ALL) patients in clinical remission retained abnormal DNA methylation patters and whether these were associated with patient outcome.MATERIALS & METHODS: We investigated CpG island methylation of genes known to exhibit hypermethylation in leukemia using quantitative pyrosequencing analysis.RESULTS: Although methylation levels were reduced in remission samples, they remained significantly higher than those seen in healthy controls. This retained methylation was not related to low levels of residual leukemia cells still present at remission. Methylation levels were also stable (or increased) during continuous remission and significantly correlated with long-term survival in adult ALL patients.CONCLUSION: This study determined that abnormalities in DNA methylation are retained during ALL remission and may represent a novel prognostic marker for adult ALL patients.",

author = "{van Otterdijk}, {Sanne D} and Jean Norden and Dickinson, {Anne M} and Pearce, {Mark S} and Relton, {Caroline L} and Mathers, {John C} and Gordon Strathdee",

year = "2015",

month = feb,

doi = "10.2217/epi.14.78",

language = "English",

volume = "7",

pages = "35--45",

journal = "Epigenomics",

issn = "1750-1911",

publisher = "Future Medicine Ltd.",

number = "1",

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Aberrations in DNA methylation are detectable during remission of acute lymphoblastic leukemia and predict patient outcome. / van Otterdijk, Sanne D; Norden, Jean; Dickinson, Anne M; Pearce, Mark S; Relton, Caroline L; Mathers, John C; Strathdee, Gordon.

In: Epigenomics, Vol. 7, No. 1, 02.2015, p. 35-45.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Aberrations in DNA methylation are detectable during remission of acute lymphoblastic leukemia and predict patient outcome

AU - van Otterdijk, Sanne D

AU - Norden, Jean

AU - Dickinson, Anne M

AU - Pearce, Mark S

AU - Relton, Caroline L

AU - Mathers, John C

AU - Strathdee, Gordon

PY - 2015/2

Y1 - 2015/2

N2 - AIM: Aberrant DNA methylation patterns are a hallmark of cancer, although the extent to which they underlie cancer development is unknown. In this study, we aimed to determine whether acute lymphoblastic leukemia (ALL) patients in clinical remission retained abnormal DNA methylation patters and whether these were associated with patient outcome.MATERIALS & METHODS: We investigated CpG island methylation of genes known to exhibit hypermethylation in leukemia using quantitative pyrosequencing analysis.RESULTS: Although methylation levels were reduced in remission samples, they remained significantly higher than those seen in healthy controls. This retained methylation was not related to low levels of residual leukemia cells still present at remission. Methylation levels were also stable (or increased) during continuous remission and significantly correlated with long-term survival in adult ALL patients.CONCLUSION: This study determined that abnormalities in DNA methylation are retained during ALL remission and may represent a novel prognostic marker for adult ALL patients.

AB - AIM: Aberrant DNA methylation patterns are a hallmark of cancer, although the extent to which they underlie cancer development is unknown. In this study, we aimed to determine whether acute lymphoblastic leukemia (ALL) patients in clinical remission retained abnormal DNA methylation patters and whether these were associated with patient outcome.MATERIALS & METHODS: We investigated CpG island methylation of genes known to exhibit hypermethylation in leukemia using quantitative pyrosequencing analysis.RESULTS: Although methylation levels were reduced in remission samples, they remained significantly higher than those seen in healthy controls. This retained methylation was not related to low levels of residual leukemia cells still present at remission. Methylation levels were also stable (or increased) during continuous remission and significantly correlated with long-term survival in adult ALL patients.CONCLUSION: This study determined that abnormalities in DNA methylation are retained during ALL remission and may represent a novel prognostic marker for adult ALL patients.

U2 - 10.2217/epi.14.78

DO - 10.2217/epi.14.78

M3 - Article (Academic Journal)

C2 - 25687464

VL - 7

SP - 35

EP - 45

JO - Epigenomics

JF - Epigenomics

SN - 1750-1911

IS - 1

ER -

Aberrations in DNA methylation are detectable during remission of acute lymphoblastic leukemia and predict patient outcome

Abstract

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MRC UoB UNITE Unit - Programme 2

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