@inbook{0fa3e11963b646bda19aa52c3ee5a2be,
title = "ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts",
abstract = "Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states. A multitude of applications in contemporary molecular and synthetic biology rely on efficient, robust and flexible methods to assemble multicomponent DNA circuits as a prerequisite to recapitulate such biological systems in vitro and in vivo. Numerous functionalities need to be combined to allow for the controlled realization of information encoded in a defined DNA circuit. Much of biological function in cells is catalyzed by multiprotein machines typically made up of many subunits. Provision of these multiprotein complexes in the test-tube is a vital prerequisite to study their structure and function, to understand biology and to develop intervention strategies to correct malfunction in disease states. ACEMBL is a technology concept that specifically addresses the requirements of multicomponent DNA assembly into multigene constructs, for gene delivery and the production of multiprotein complexes in high-throughput. ACEMBL is applicable to prokaryotic and eukaryotic expression hosts, to accelerate basic and applied research and development. The ACEMBL concept, reagents, protocols and its potential are reviewed in this contribution.",
keywords = "Gene delivery, High-throughput, Automation, Robotics, Structural proteomics, Protein complexes, Membrane proteins, Synthetic biology, Metabolic engineering",
author = "Yan Nie and Maxime Chaillet and Christian Becke and Matthias Haffke and Martin Pelosse and Daniel Fitzgerald and Ian Collinson and Christiane Schaffitzel and Imre Berger",
year = "2016",
month = may,
day = "11",
doi = "10.1007/978-3-319-27216-0_3",
language = "English",
isbn = "9783319272146",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer International Publishing AG",
pages = "27--42",
editor = "Vega, {M. Cristina}",
booktitle = "Advanced Technologies for Protein Complex Production and Characterization",
address = "Switzerland",
}