ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts

Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel, Imre Berger

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

11 Citations (Scopus)
383 Downloads (Pure)

Abstract

Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states. A multitude of applications in contemporary molecular and synthetic biology rely on efficient, robust and flexible methods to assemble multicomponent DNA circuits as a prerequisite to recapitulate such biological systems in vitro and in vivo. Numerous functionalities need to be combined to allow for the controlled realization of information encoded in a defined DNA circuit. Much of biological function in cells is catalyzed by multiprotein machines typically made up of many subunits. Provision of these multiprotein complexes in the test-tube is a vital prerequisite to study their structure and function, to understand biology and to develop intervention strategies to correct malfunction in disease states. ACEMBL is a technology concept that specifically addresses the requirements of multicomponent DNA assembly into multigene constructs, for gene delivery and the production of multiprotein complexes in high-throughput. ACEMBL is applicable to prokaryotic and eukaryotic expression hosts, to accelerate basic and applied research and development. The ACEMBL concept, reagents, protocols and its potential are reviewed in this contribution.
Original languageEnglish
Title of host publicationAdvanced Technologies for Protein Complex Production and Characterization
EditorsM. Cristina Vega
PublisherSpringer International Publishing AG
Pages27-42
Number of pages16
ISBN (Electronic)9783319272160
ISBN (Print)9783319272146
DOIs
Publication statusPublished - 11 May 2016

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer International Publishing
Volume896
ISSN (Print)0065-2598

Structured keywords

  • Bristol BioDesign Institute

Keywords

  • Gene delivery
  • High-throughput
  • Automation
  • Robotics
  • Structural proteomics
  • Protein complexes
  • Membrane proteins
  • Synthetic biology
  • Metabolic engineering

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