Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek’s Disease

Ayumi Matsuyama-Kato; Bahram Shojadoost; Nitish Boodhoo; Sugandha Raj; Mohammadali Alizadeh; Fatemeh Fazel; Charlotte Fletcher; Jiayu Zheng; Bhavya Gupta; Mohamed Faizal Abdul-Careem; Brandon L. Plattner; Shahriar Behboudi; Shayan Sharif

doi:10.3390/v15020285

Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek’s Disease

Ayumi Matsuyama-Kato, Bahram Shojadoost, Nitish Boodhoo, Sugandha Raj, Mohammadali Alizadeh, Fatemeh Fazel, Charlotte Fletcher, Jiayu Zheng, Bhavya Gupta, Mohamed Faizal Abdul-Careem, Brandon L. Plattner, Shahriar Behboudi, Shayan Sharif

Research output: Contribution to journal › Article (Academic Journal) › peer-review

14 Citations (Scopus)

Abstract

Gamma delta (γδ) T cells play a significant role in the prevention of viral infection and tumor surveillance in mammals. Although the involvement of γδ T cells in Marek’s disease virus (MDV) infection has been suggested, their detailed contribution to immunity against MDV or the progression of Marek’s disease (MD) remains unknown. In the current study, T cell receptor (TCR)γδ-activated peripheral blood mononuclear cells (PBMCs) were infused into recipient chickens and their effects were examined in the context of tumor formation by MDV and immunity against MDV. We demonstrated that the adoptive transfer of TCRγδ-activated PBMCs reduced virus replication in the lungs and tumor incidence in MDV-challenged chickens. Infusion of TCRγδ-activated PBMCs induced IFN-γ-producing γδ T cells at 10 days post-infection (dpi), and degranulation activity in circulating γδ T cell and CD8α+ γδ T cells at 10 and 21 dpi in MDV-challenged chickens. Additionally, the upregulation of IFN-γ and granzyme A gene expression at 10 dpi was significant in the spleen of the TCRγδ-activated PBMCs-infused and MDV-challenged group compared to the control group. Taken together, our results revealed that TCRγδ stimulation promotes the effector function of chicken γδ T cells, and these effector γδ T cells may be involved in protection against MD.

Original language	English
Article number	285
Journal	Viruses
Volume	15
Issue number	2
DOIs	https://doi.org/10.3390/v15020285
Publication status	Published - 19 Jan 2023

Bibliographical note

Funding Information:
This work was supported by the Ontario Ministry of Agriculture, Food and Rural Affairs (funding number 030157); the Natural Sciences and Engineering Research Council of Canada (funding number 400561); and the University of Guelph’s Food from Thought initiative (funding number 499142). Shahriar Behboudi was funded by U.K. Research and Innovation Biotechnology and Biological Sciences Research Council Grant BBS/E/I/00007030.

Publisher Copyright:
© 2023 by the authors.

Access to Document

10.3390/v15020285Licence: CC BY

Handle.net

Persistent link

Cite this

@article{fbd00c380d0e4db6a11a9fa4e4507458,

title = "Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek{\textquoteright}s Disease",

abstract = "Gamma delta (γδ) T cells play a significant role in the prevention of viral infection and tumor surveillance in mammals. Although the involvement of γδ T cells in Marek{\textquoteright}s disease virus (MDV) infection has been suggested, their detailed contribution to immunity against MDV or the progression of Marek{\textquoteright}s disease (MD) remains unknown. In the current study, T cell receptor (TCR)γδ-activated peripheral blood mononuclear cells (PBMCs) were infused into recipient chickens and their effects were examined in the context of tumor formation by MDV and immunity against MDV. We demonstrated that the adoptive transfer of TCRγδ-activated PBMCs reduced virus replication in the lungs and tumor incidence in MDV-challenged chickens. Infusion of TCRγδ-activated PBMCs induced IFN-γ-producing γδ T cells at 10 days post-infection (dpi), and degranulation activity in circulating γδ T cell and CD8α+ γδ T cells at 10 and 21 dpi in MDV-challenged chickens. Additionally, the upregulation of IFN-γ and granzyme A gene expression at 10 dpi was significant in the spleen of the TCRγδ-activated PBMCs-infused and MDV-challenged group compared to the control group. Taken together, our results revealed that TCRγδ stimulation promotes the effector function of chicken γδ T cells, and these effector γδ T cells may be involved in protection against MD.",

author = "Ayumi Matsuyama-Kato and Bahram Shojadoost and Nitish Boodhoo and Sugandha Raj and Mohammadali Alizadeh and Fatemeh Fazel and Charlotte Fletcher and Jiayu Zheng and Bhavya Gupta and Abdul-Careem, {Mohamed Faizal} and Plattner, {Brandon L.} and Shahriar Behboudi and Shayan Sharif",

note = "Funding Information: This work was supported by the Ontario Ministry of Agriculture, Food and Rural Affairs (funding number 030157); the Natural Sciences and Engineering Research Council of Canada (funding number 400561); and the University of Guelph{\textquoteright}s Food from Thought initiative (funding number 499142). Shahriar Behboudi was funded by U.K. Research and Innovation Biotechnology and Biological Sciences Research Council Grant BBS/E/I/00007030. Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = jan,

day = "19",

doi = "10.3390/v15020285",

language = "English",

volume = "15",

journal = "Viruses",

issn = "1999-4915",

publisher = "MDPI AG",

number = "2",

}

TY - JOUR

T1 - Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek’s Disease

AU - Matsuyama-Kato, Ayumi

AU - Shojadoost, Bahram

AU - Boodhoo, Nitish

AU - Raj, Sugandha

AU - Alizadeh, Mohammadali

AU - Fazel, Fatemeh

AU - Fletcher, Charlotte

AU - Zheng, Jiayu

AU - Gupta, Bhavya

AU - Abdul-Careem, Mohamed Faizal

AU - Plattner, Brandon L.

AU - Behboudi, Shahriar

AU - Sharif, Shayan

N1 - Funding Information: This work was supported by the Ontario Ministry of Agriculture, Food and Rural Affairs (funding number 030157); the Natural Sciences and Engineering Research Council of Canada (funding number 400561); and the University of Guelph’s Food from Thought initiative (funding number 499142). Shahriar Behboudi was funded by U.K. Research and Innovation Biotechnology and Biological Sciences Research Council Grant BBS/E/I/00007030. Publisher Copyright: © 2023 by the authors.

PY - 2023/1/19

Y1 - 2023/1/19

N2 - Gamma delta (γδ) T cells play a significant role in the prevention of viral infection and tumor surveillance in mammals. Although the involvement of γδ T cells in Marek’s disease virus (MDV) infection has been suggested, their detailed contribution to immunity against MDV or the progression of Marek’s disease (MD) remains unknown. In the current study, T cell receptor (TCR)γδ-activated peripheral blood mononuclear cells (PBMCs) were infused into recipient chickens and their effects were examined in the context of tumor formation by MDV and immunity against MDV. We demonstrated that the adoptive transfer of TCRγδ-activated PBMCs reduced virus replication in the lungs and tumor incidence in MDV-challenged chickens. Infusion of TCRγδ-activated PBMCs induced IFN-γ-producing γδ T cells at 10 days post-infection (dpi), and degranulation activity in circulating γδ T cell and CD8α+ γδ T cells at 10 and 21 dpi in MDV-challenged chickens. Additionally, the upregulation of IFN-γ and granzyme A gene expression at 10 dpi was significant in the spleen of the TCRγδ-activated PBMCs-infused and MDV-challenged group compared to the control group. Taken together, our results revealed that TCRγδ stimulation promotes the effector function of chicken γδ T cells, and these effector γδ T cells may be involved in protection against MD.

AB - Gamma delta (γδ) T cells play a significant role in the prevention of viral infection and tumor surveillance in mammals. Although the involvement of γδ T cells in Marek’s disease virus (MDV) infection has been suggested, their detailed contribution to immunity against MDV or the progression of Marek’s disease (MD) remains unknown. In the current study, T cell receptor (TCR)γδ-activated peripheral blood mononuclear cells (PBMCs) were infused into recipient chickens and their effects were examined in the context of tumor formation by MDV and immunity against MDV. We demonstrated that the adoptive transfer of TCRγδ-activated PBMCs reduced virus replication in the lungs and tumor incidence in MDV-challenged chickens. Infusion of TCRγδ-activated PBMCs induced IFN-γ-producing γδ T cells at 10 days post-infection (dpi), and degranulation activity in circulating γδ T cell and CD8α+ γδ T cells at 10 and 21 dpi in MDV-challenged chickens. Additionally, the upregulation of IFN-γ and granzyme A gene expression at 10 dpi was significant in the spleen of the TCRγδ-activated PBMCs-infused and MDV-challenged group compared to the control group. Taken together, our results revealed that TCRγδ stimulation promotes the effector function of chicken γδ T cells, and these effector γδ T cells may be involved in protection against MD.

UR - http://dx.doi.org/10.3390/v15020285

U2 - 10.3390/v15020285

DO - 10.3390/v15020285

M3 - Article (Academic Journal)

C2 - 36851499

SN - 1999-4915

VL - 15

JO - Viruses

JF - Viruses

IS - 2

M1 - 285

ER -

Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek’s Disease

Abstract

Bibliographical note

Access to Document

Handle.net

Other files and links

Fingerprint

Cite this