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Obesity is associated with changes in the secretome of adipose tissue (AT), which affects the vasculature through endocrine and paracrine mechanisms. Wingless-related integration site 5A(WNT5A) and secreted frizzled related protein 5 (SFRP5), adipokines that regulate noncanonical Wnt signaling, are dysregulated in obesity. We hypothesised that WNT5A released from AT exerts endocrine and paracrine effects on the arterial wall through non-canonical RAC1-mediated Wnt signaling. In a cohort of 1,004 humans with atherosclerosis, obesity was associated with increased WNT5A bioavailability in the circulation and the AT, higher expression of WNT5A receptors Frizzled 2 and 5 in the human arterial wall, and increased vascular oxidative stress due to activation of NADPH oxidases. Plasma concentration of WNT5A was elevated in patients with coronary artery disease compared to matched controls and was independently associated with calcified coronary plaque progression. We further demonstrated that WNT5A induces arterial oxidative stress and redox-sensitive migration of vascular smooth muscle cells via Frizzled 2-mediated activation of a previously uncharacterised pathway involving the deubiquitinating enzyme ubiquitin-specific protease 17 (USP17) and the GTPase RAC1. Our study identifies WNT5A and its downstream vascular signaling as a link between obesity and vascular disease pathogenesis, with translational implications in humans.
|Number of pages||17|
|Journal||Science Translational Medicine|
|Publication status||Published - 18 Sep 2019|
FingerprintDive into the research topics of 'Adipose tissue-derived WNT5A regulates vascular redox signaling in obesity via USP17//RAC1-mediated activation of NADPH oxidases'. Together they form a unique fingerprint.
- 1 Finished
Professor Sarah J George
- Health Sciences Faculty Office - Interim Dean of the Faculty of Health Sciences
- Fundamental Bioscience
Person: Academic , Member