Adult zebrafish as a model system for cutaneous wound-healing research

Rebecca Richardson, Krasimir Slanchev, Christopher Kraus, Philipp Knyphausen, Sabine Eming, Matthias Hammerschmidt

Research output: Contribution to journalArticle (Academic Journal)peer-review

208 Citations (Scopus)


Upon injury, the skin must quickly regenerate to regain its barrier function. In mammals, wound healing is rapid and scar free during embryogenesis, whereas in adults it involves multiple steps including blood clotting, inflammation, re-epithelialization, vascularization, and granulation tissue formation and maturation, resulting in a scar. We have established a rapid and robust method to introduce full-thickness wounds onto the flank of adult zebrafish, and show that apart from external fibrin clot formation, all steps of adult mammalian wound repair also exist in zebrafish. Wound re-epithelialization is extremely rapid and initiates with no apparent lag phase, subsequently followed by the immigration of inflammatory cells and the formation of granulation tissue, consisting of macrophages, fibroblasts, blood vessels, and collagen. The granulation tissue later regresses, resulting in minimal scar formation. Studies after chemical treatment or with transgenic fish further suggest that wound re-epithelialization occurs independently of inflammation and fibroblast growth factor signaling, whereas both are essential for fibroblast recruitment and granulation tissue formation. Together, these results demonstrate that major steps and principles of cutaneous wound healing are conserved among adult mammals and adult zebrafish, making zebrafish a valuable model for studying vertebrate skin repair.

Original languageEnglish
Pages (from-to)1655-65
Number of pages11
JournalJournal of Investigative Dermatology
Issue number6
Publication statusPublished - Jun 2013


  • Animals
  • Animals, Genetically Modified
  • Blood Coagulation
  • Cicatrix
  • Dermatitis
  • Disease Models, Animal
  • Epithelial Cells
  • Fibroblast Growth Factors
  • Granulation Tissue
  • Neovascularization, Physiologic
  • Signal Transduction
  • Skin
  • Wound Healing
  • Zebrafish


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