Abstract
Most membrane-proteins exist in complexes rather than isolated entities. To fully understand their biological function it is essential to study the intact membrane-protein assemblies. The overexpression and purification of many essential membrane-protein complexes is still a considerable and often unsurmountable challenge. In these cases, extraction from source is the only option for many large multi-subunit cellular machines. Here, we describe recent advances in overexpression of multi-subunit membrane-protein complexes, the strategies to stabilize these complexes and highlight major achievements in membrane-protein structural research that were facilitated by the prospect of achieving subnanometer to near-atomic resolution by electron cryo-microscopy.
Original language | English |
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Pages (from-to) | 123-30 |
Number of pages | 8 |
Journal | Current Opinion in Structural Biology |
Volume | 32 |
DOIs | |
Publication status | Published - Jun 2015 |
Bibliographical note
Copyright © 2015 Elsevier Ltd. All rights reserved.Research Groups and Themes
- Bristol BioDesign Institute
Keywords
- synthetic biology