Age-related changes in the renal dopaminergic system and expression of renal amino acid transporters in WKY and SHR rats

Vanda Pinto, João Amaral, Elisabete Silva, Sónia Simão, José Miguel Cabral, Joana Afonso, Maria Paula Serrão, Pedro Gomes, Maria João Pinho, Patrício Soares-da-Silva

Research output: Contribution to journalArticle (Academic Journal)peer-review

10 Citations (Scopus)

Abstract

This study examined age-related changes in renal dopaminergic activity and expression of amino acid transporters potentially involved in renal tubular uptake of l-DOPA in Wistar Kyoto (WKY) and spontaneously hypertensive rats. Aging (from 13 to 91 weeks) was accompanied by increases in systolic blood pressure (SBP) in both WKY and SHR. The sum of urinary dopamine and DOPAC and the urinary dopamine/l-DOPA ratio were increased in aged SHR but not in aged WKY. The urinary dopamine/renal delivery of l-DOPA ratio was increased in both rat strains with aging. LAT2 abundance was increased in aged WKY and SHR. The expression of 4F2hc was markedly elevated in aged SHR but not in aged WKY. ASCT2 was upregulated in both aged WKY and SHR. Plasma aldosterone levels and urinary noradrenaline levels were increased in aged WKY and SHR though levels of both entities were more elevated in aged SHR. Activation of the renal dopaminergic system is more pronounced in aged SHR than in aged WKY and is associated with an upregulation of renal cortical ASCT2 in WKY and of LAT2/4F2hc and ASCT2 in SHR. This activation may be the consequence of a counter-regulatory mechanism for stimuli leading to sodium reabsorption.

Original languageEnglish
Pages (from-to)298-304
Number of pages7
JournalMechanisms of Ageing and Development
Volume132
Issue number6-7
DOIs
Publication statusPublished - 28 Jun 2011

Bibliographical note

Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Keywords

  • 3,4-Dihydroxyphenylacetic Acid
  • Aging
  • Amino Acid Transport System ASC
  • Amino Acid Transport System y+
  • Animals
  • Antigens, CD98 Light Chains
  • Dopamine
  • Kidney Cortex
  • Male
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY

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