Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex

Lisa Gianesello, Giovanna Priante, Monica Ceol, Claudia M Radu, Moin A Saleem, Paolo Simioni, Liliana Terrin, Franca Anglani, Dorella Del Prete

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to internalizing albumin through a receptor-mediated mechanism even after exposure to low doses of albumin. We show that these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which are partners in the tubular machinery. Exposing human podocytes to albumin overload prompted an increase in CUBILIN, AMNIONLESS and CLCN5 gene expression. Inhibiting cubilin led to a reduction in albumin uptake, highlighting its importance in this mechanism. We demonstrated that human podocytes are committed to performing endocytosis via a receptor-mediated mechanism even in the presence of low doses of albumin. We also disclosed that protein overload first acts on the expression of the cubilin-amnionless (CUBAM) complex in these cells, then involves the ClC-5 channel, providing the first evidence for a possible role of the CUBAM complex in albumin endocytosis in human podocytes.

Original languageEnglish
Pages (from-to)13705
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 20 Oct 2017

Keywords

  • Journal Article

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