Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

Graham Fairfoul; Lynne I McGuire; Suvankar Pal; James W Ironside; Juliane Neumann; Sharon Christie; Catherine Joachim; Margaret Esiri; Samuel G Evetts; Michal Rolinski; Fahd Baig; Claudio Ruffmann; Richard Wade-Martins; Michele T M Hu; Laura Parkkinen; Alison J E Green

doi:10.1002/acn3.338

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

Graham Fairfoul, Lynne I McGuire, Suvankar Pal, James W Ironside, Juliane Neumann, Sharon Christie, Catherine Joachim, Margaret Esiri, Samuel G Evetts, Michal Rolinski, Fahd Baig, Claudio Ruffmann, Richard Wade-Martins, Michele T M Hu, Laura Parkkinen, Alison J E Green

Bristol Medical School (THS)

Research output: Contribution to journal › Article (Academic Journal) › peer-review

344 Citations (Scopus)

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Abstract

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

Original language	English
Pages (from-to)	812-818
Number of pages	7
Journal	Annals of Clinical and Translational Neurology
Volume	3
Issue number	10
Early online date	28 Aug 2016
DOIs	https://doi.org/10.1002/acn3.338
Publication status	Published - Oct 2016

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Dr Harry Rolinski
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- Bristol Medical School (THS) - Honorary Lecturer
- Bristol Neuroscience
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Fairfoul, G., McGuire, L. I., Pal, S., Ironside, J. W., Neumann, J., Christie, S., Joachim, C., Esiri, M., Evetts, S. G., Rolinski, M., Baig, F., Ruffmann, C., Wade-Martins, R., Hu, M. T. M., Parkkinen, L., & Green, A. J. E. (2016). Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Annals of Clinical and Translational Neurology, 3(10), 812-818. https://doi.org/10.1002/acn3.338

@article{ad7fdf4265e84adaa4e6dc2b03ce0686,

title = "Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies",

abstract = "We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.",

author = "Graham Fairfoul and McGuire, {Lynne I} and Suvankar Pal and Ironside, {James W} and Juliane Neumann and Sharon Christie and Catherine Joachim and Margaret Esiri and Evetts, {Samuel G} and Michal Rolinski and Fahd Baig and Claudio Ruffmann and Richard Wade-Martins and Hu, {Michele T M} and Laura Parkkinen and Green, {Alison J E}",

year = "2016",

month = oct,

doi = "10.1002/acn3.338",

language = "English",

volume = "3",

pages = "812--818",

journal = "Annals of Clinical and Translational Neurology",

issn = "2328-9503",

publisher = "John Wiley & Sons, Ltd.",

number = "10",

}

Fairfoul, G, McGuire, LI, Pal, S, Ironside, JW, Neumann, J, Christie, S, Joachim, C, Esiri, M, Evetts, SG, Rolinski, M, Baig, F, Ruffmann, C, Wade-Martins, R, Hu, MTM, Parkkinen, L & Green, AJE 2016, 'Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies', Annals of Clinical and Translational Neurology, vol. 3, no. 10, pp. 812-818. https://doi.org/10.1002/acn3.338

TY - JOUR

T1 - Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

AU - Fairfoul, Graham

AU - McGuire, Lynne I

AU - Pal, Suvankar

AU - Ironside, James W

AU - Neumann, Juliane

AU - Christie, Sharon

AU - Joachim, Catherine

AU - Esiri, Margaret

AU - Evetts, Samuel G

AU - Rolinski, Michal

AU - Baig, Fahd

AU - Ruffmann, Claudio

AU - Wade-Martins, Richard

AU - Hu, Michele T M

AU - Parkkinen, Laura

AU - Green, Alison J E

PY - 2016/10

Y1 - 2016/10

N2 - We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

AB - We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

U2 - 10.1002/acn3.338

DO - 10.1002/acn3.338

M3 - Article (Academic Journal)

C2 - 27752516

SN - 2328-9503

VL - 3

SP - 812

EP - 818

JO - Annals of Clinical and Translational Neurology

JF - Annals of Clinical and Translational Neurology

IS - 10

ER -

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

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