Altered expression of auxin-binding protein 1 affects cell expansion and auxin pool size in tobacco cells

J-GN Chen, S Wang, CM Lazarus, RM Napier, AM Jones

Research output: Contribution to journalArticle (Academic Journal)

8 Citations (Scopus)

Abstract

Auxin-binding protein 1 (ABP1) has an essential role in auxin-dependent cell expansion, but its mechanisms of action remain unknown. Our previous study showed that ABP1-mediated cell expansion is auxin concentration dependent. However, auxin distribution in plant tissue is heterogeneous, complicating the interpretation of ABP1 function. In this study, we used cells in culture that have altered expression of ABP1 to address the mechanism of ABP1 action at the cellular level, because cells in culture have homogeneous cell types and could potentially circumvent the heterogeneous auxin-distributions inherent in plant tissues. We found that cells overexpressing ABP1 had altered sensitivity to auxin and were larger, with nuclei that have undergone endoreduplication, a finding consistent with other data that support an auxin extracellular receptor role for ABP1. These cells also had a higher free auxin pool size, which cannot be explained by altered auxin transport. In cells lacking detectable ABP1, a higher rate of auxin metabolism was observed. The results suggest that ABP1 has, beyond its proposed role as an auxin extracellular receptor, a role in mediating auxin availability.
Translated title of the contributionAltered expression of auxin-binding protein 1 affects cell expansion and auxin pool size in tobacco cells
Original languageEnglish
Pages (from-to)69 - 78
Number of pages10
JournalJournal of Plant Growth Regulation
Volume25 (1)
DOIs
Publication statusPublished - Mar 2006

Bibliographical note

Publisher: Springer

Fingerprint Dive into the research topics of 'Altered expression of auxin-binding protein 1 affects cell expansion and auxin pool size in tobacco cells'. Together they form a unique fingerprint.

  • Cite this